RESUMO
OBJECTIVE: We wanted to compare the human neural stem cell (hNSC) labeling efficacy of different superparamagnetic iron oxide nanoparticles (SPIONs), namely, ferumoxides, monocrystalline iron oxide (MION), cross-linked iron oxide (CLIO)-NH2 and tat-CLIO. MATERIALS AND METHODS: The hNSCs (5x105 HB1F3 cells/ml) were incubated for 24 hr in cell culture media that contained 25 microgram/ml of ferumoxides, MION or CLIO-NH2, and with or without poly-L-lysine (PLL) and tat-CLIO. The cellular iron uptake was analyzed qualitatively with using a light microscope and this was quantified via atomic absorption spectrophotometry. The visibility of the labeled cells was assessed with MR imaging. RESULTS: The incorporation of SPIONs into the hNSCs did not affect the cellular proliferations and viabilities. The hNSCs labeled with tat-CLIO showed the longest retention, up to 72 hr, and they contained 2.15+/-0.3 pg iron/cell, which are 59 fold, 430 fold and six fold more incorporated iron than that of the hNSCs labeled with ferumoxides, MION or CLIO-NH2, respectively. However, when PLL was added, the incorporation of ferumoxides, MION or CLIO-NH2 into the hNSCs was comparable to that of tat-CLIO. CONCLUSION: For MR imaging, hNSCs can be efficiently labeled with tat-CLIO alone or with a combination of ferumoxides, MION, CLIO-NH2 and the transfection agent PLL.
Assuntos
Humanos , Células Cultivadas , Meios de Contraste/síntese química , Reagentes de Ligações Cruzadas/química , Compostos Férricos/química , Óxido Ferroso-Férrico/síntese química , Produtos do Gene tat/química , Ferro/farmacocinética , Imageamento por Ressonância Magnética/métodos , Nanopartículas , Tubo Neural , Óxidos/farmacocinética , Imagens de Fantasmas , Polilisina/farmacocinética , Espectrofotometria Atômica , Coloração e Rotulagem/métodos , Células-Tronco/citologia , Fatores de Tempo , TransfecçãoRESUMO
Los nuevos Medios de Contraste Radiopacos Iodados, de Baja Osmolaridad; y en particular los No Iónicos, han llamado la atención por los beneficios y mayor tolerancia que ofrecen en los estudios radiológicos (Urografía, Angiografía, Tomografía Computada, etc) disminuyendo los efectos colaterales y toxicidad. El cambio radical hacia el uso de los Medios de contraste no iónicos, conlleva la suficiente información que deben recibir los médicos radiólogos, para familiarizarse con estos nuevos productos y considerar asi mismo la relación riesgio/beneficio de sus pacientes