Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Adicionar filtros








Intervalo de ano
1.
Chinese journal of integrative medicine ; (12): 208-214, 2017.
Artigo em Inglês | WPRIM | ID: wpr-327221

RESUMO

<p><b>OBJECTIVES</b>To study the effect of Wenhua Juanbi Recipe (, WJR) on expression of receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), and tumor necrosis factor receptor superfamily member 14 (TNFRSF14, also known as LIGHT) in rats with collagen-induced arthritis (CIA).</p><p><b>METHODS</b>CIA rats were generated by subcutaneous injection of bovine collagen type-II at the tail base. Sixty CIA rats were randomly assigned (10 animals/group) to: model, methotrexate (MTX)-treated (0.78 mg/kg body weight), and WJR-treated (22.9 g/kg) groups. Healthy normal rats (n=10) were used as the normal control. Treatments or saline were administered once daily by oral gavage. Rats were sacrifificed at day 28 post-treatment and knee synovium and peripheral blood serum were collected. Toe swelling degree and expression of RANKL, OPG, and LIGHT were determined by Western blot and immunohistochemistry.</p><p><b>RESULTS</b>Compared with the normal group, toe swelling degree was signifificantly increased in the model group (P<0.01). After treatment, toe swelling degree decreased signifificantly in the WJR and MTX groups compared with the model group (P<0.01). Compared with the normal group, expression of RANKL and LIGHT were signifificantly increased and OPG signifificantly decreased in peripheral blood and synovium of the model group (P<0.01). Conversely, RANKL and LIGHT expression were signifificantly reduced and OPG increased in the WJR and MTX groups compared with the model group (P<0.01). No statistically significant difference existed between WJR and MTX groups.</p><p><b>CONCLUSION</b>WJR likely acts by reducing RANKL expression and increasing OPG expression, thus inhibiting RANKL/RANK interaction and reducing LIGHT expression, thereby inhibiting osteoclast formation/activation to block bone erosion.</p>


Assuntos
Animais , Bovinos , Masculino , Artrite Experimental , Tratamento Farmacológico , Metabolismo , Western Blotting , Medicamentos de Ervas Chinesas , Farmacologia , Usos Terapêuticos , Imuno-Histoquímica , Osteoprotegerina , Metabolismo , Ligante RANK , Metabolismo , Ratos Wistar , Membro 14 de Receptores do Fator de Necrose Tumoral , Metabolismo , Membrana Sinovial , Patologia
2.
Experimental & Molecular Medicine ; : 149-158, 2012.
Artigo em Inglês | WPRIM | ID: wpr-93415

RESUMO

The development of gastric cancer (GC) is closely related to chronic inflammation caused by Helicobacter pylori infection, and herpes virus entry mediator (HVEM) is a receptor expressed on the surface of leukocytes that mediates potent inflammatory responses in animal models. However, the role of HVEM in human GC has not been studied. Previously, we showed that the interaction of HVEM on human leukocytes with its ligand LIGHT induces intracellular calcium mobilization, which results in inflammatory responses including induction of proinflammatory cytokine production and anti-bacterial activities. In this study, we report that leukocytes from GC patients express lower levels of membrane HVEM (mHVEM) and have lower LIGHT-induced bactericidal activities than those from healthy controls (HC). In contrast, levels of soluble HVEM (sHVEM) in the sera of GC patients were significantly higher than in those of HC. We found that monocyte membrane-bound HVEM is released into the medium when cells are activated by proinflammatory cytokines such as TNF-alpha and IL-8, which are elevated in the sera of GC patients. mHVEM level dropped in parallel with the release of sHVEM, and release was completely blocked by the metalloprotease inhibitor, GM6001. We also found that the low level of mHVEM on GC patient leukocytes was correlated with low LIGHT-induced bactericidal activities against H. pylori and S. aureus and production of reactive oxygen species. Our results indicate that mHVEM on leukocytes and sHVEM in sera may contribute to the development and/or progression of GC.


Assuntos
Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Células Cultivadas , Ensaio de Imunoadsorção Enzimática , Citometria de Fluxo , Monócitos/metabolismo , Neutrófilos/metabolismo , Espécies Reativas de Oxigênio/metabolismo , Membro 14 de Receptores do Fator de Necrose Tumoral/sangue , Neoplasias Gástricas/sangue , Membro 14 da Superfamília de Ligantes de Fatores de Necrose Tumoral/sangue
3.
Chinese Journal of Oncology ; (12): 187-191, 2012.
Artigo em Chinês | WPRIM | ID: wpr-335316

RESUMO

<p><b>OBJECTIVE</b>To generate an oncolytic herpes simplex virus (oHSV) permissive mouse melanoma cell line B16RHSV, preserving the tumorigenic ability in syngeneic mice.</p><p><b>METHODS</b>The herpes simplex virus entry mediator (HVEM) gene was amplified by PCR from human melanoma cell line A375, and cloned into pGEM-T Easy vector for sequencing. The HVEM gene was then cloned into pcDNA3 vector to generate pcDNA3-HVEM for transfection of mouse melanoma cell line B16-F10 cells. After that, the putative transfected cells were selected in full growth medium containing G418. The HVEM-expressing cells were isolated by immunomagnetic bead separation. The mouse melanoma cell line expressing oHSV receptor-HVEM, designated as B16RHSV, was generated. The permissibility of B16RHSV cells to oHSV infection was examined with green fluorescence protein (GFP)-expressing oHSV (oHSVGFP). To investigate the tumorigenic ability of both cells in vivo, 2×10(5) cells in 100 µl were subcutaneously inoculated into the right flanks of C57/BL mice.</p><p><b>RESULTS</b>In vitro, the B16RHSV mouse melanoma cells were shown by fluorescence microscopy capable of being infected by oHSVGFP. In vivo, the B16RHSV cells, like their wild type counterpart, grew to form melanoma in syngeneic mice.</p><p><b>CONCLUSION</b>A herpes simplex virus-permissive mouse melanoma cell line was established. Its tumorigenicity remained unchanged.</p>


Assuntos
Animais , Feminino , Humanos , Camundongos , Linhagem Celular Tumoral , Amplificação de Genes , Vetores Genéticos , Herpesvirus Humano 1 , Genética , Fisiologia , Melanoma , Patologia , Virologia , Camundongos Endogâmicos C57BL , Transplante de Neoplasias , Plasmídeos , Membro 14 de Receptores do Fator de Necrose Tumoral , Genética , Metabolismo , Transfecção , Carga Tumoral
4.
Experimental & Molecular Medicine ; : 805-810, 2010.
Artigo em Inglês | WPRIM | ID: wpr-122578

RESUMO

Tumor necrosis factor receptor-related 2 (TR2, HVEM or TNFRSF-14) plays an important role in immune responses, however, the mechanisms regulating its expression are unclear. To understand the control of TR2 gene expression, we studied the upstream region of the gene. Gel supershift assays revealed inducible binding of nuclear factor of activated T cells (NFAT) to a putative NFAT site within the TR2 promoter. Furthermore, cotransfection of a dominant negative NFAT construct, or siRNA for NFAT, resulted in increased expression of a TR2 reporter gene. Our findings demonstrate that NFAT negatively regulates TR2 expression in activated T cells.


Assuntos
Animais , Camundongos , Sequência de Bases , Linfócitos T CD4-Positivos/metabolismo , Células Cultivadas , Regulação para Baixo , Camundongos Endogâmicos C57BL , Dados de Sequência Molecular , Fatores de Transcrição NFATC/fisiologia , Membro 14 de Receptores do Fator de Necrose Tumoral/biossíntese , Linfócitos T/metabolismo
5.
Experimental & Molecular Medicine ; : 501-508, 2003.
Artigo em Inglês | WPRIM | ID: wpr-197472

RESUMO

Herpes virus entry mediator (HVEM) is a newly discovered member of the tumor necrosis factor receptor (TNFR) superfamily that has a role in herpes simplex virus entry, in T cell activation and in tumor immunity. We generated mAb against HVEM and detected soluble HVEM (SHVEM) in the sera of patients with various autoimmune diseases. HVEM was constitutively expressed on CD4(+)and CD8(+)T cells, CD19(+)B cells, CD14(+)monocytes, neutrophils and dendritic cells. In three-way MLR, mAb 122 and 139 were agonists and mAb 108 had blocking activity. An ELISA was developed to detect sHVEM in patient sera. sHVEM levels were elevated in sera of patients with allergic asthma, atopic dermatitis and rheumatoid arthritis. The mAbs discussed here may be useful for studies of the role of HVEM in immune responses. Detection of soluble HVEM might have diagnostic and prognostic value in certain immunological disorders.


Assuntos
Animais , Feminino , Humanos , Camundongos , Anticorpos Monoclonais/imunologia , Especificidade de Anticorpos , Artrite Reumatoide/sangue , Asma/sangue , Doenças Autoimunes/sangue , Divisão Celular , Linhagem Celular , Dermatite Atópica/sangue , Citometria de Fluxo , Hipersensibilidade/sangue , Teste de Cultura Mista de Linfócitos , Camundongos Endogâmicos BALB C , Receptores do Fator de Necrose Tumoral/sangue , Membro 14 de Receptores do Fator de Necrose Tumoral , Receptores Virais/sangue , Solubilidade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA