Efecto antiespasmódico de Rubus roseus y Mentha pulegium sobre íleon aislado de cobayo (Cavia porcellus) / Antispasmodic effect of Rubus roseus and Mentha pulegium on guinea pig (Cavia porcellus) isolated ileum

Objetivo. El objetivo de este estudio fue investigar el potencial efecto antiespasmódico de Rubus roseus y Mentha pulegium en íleon aislado de cobayo (Cavia porcellus) Materiales y Métodos. Los segmentos de íleon se precontrajeron con acetilcolina (ACh; 4 × 10−6 M) y cloruro de potasio (KCl; 4 × 10-4 M) luego se evaluó la respuesta de los decoctos de Rubus roseus (Rr) Mentha pulegium (Mp) en el baño de órganos aislado en concentraciones crecientes (no acumulativas), como controles positivos se utilizaron nifedipino 5 × 10-8 M (antagonista de canales de Ca2+ voltaje dependientes); atropina sulfato 5,5 × 10-9 M (antagonista muscarínico no selectivo), y N-butilbromuro de hioscina 3 x 10-3 M (antagonista muscarínico M3 selectivo). Se cuantificó la amplitud y la frecuencia de contracción (durante diez minutos) después de la administración de los decoctos. Resultados. Los decoctos de Rubus roseus y Mentha pulegium reducen las contracciones inducidas por acetilcolina (p < 0,001) y KCl (p < 0,01) similares a nifedipino (Mp: 4 y 8 mg/mL); atropina sulfato (Rr: 0,2-4 mg/mL; Mp: 0,8 y 1,6 mg/mL) y N-butilbromuro de hioscina (Mp: 2,4 y 8 mg/mL). Por otro lado, ambos decoctos reducen la frecuencia de contracción inducidas por Ach (p < 0,001) y KCl (p < 0,001). Conclusión. Los decoctos de Rubus roseus y Mentha pulegium tienen efecto antiespasmódico en íleon aislado de cobayo (Cavia porcellus).

Objective. This study aimed to investigate the potential antispasmodic effect of Rubus roseus and Mentha pulegium on isolated guinea pig (Cavia porcellus) ileum. Materials and Methods. The ileum segments were pre-contracted with acetylcholine (ACh, 4 × 10-6 M) and potassium chloride (KCl, 4 × 10-4 M) then the response of the Rubus roseus (Rr) and Mentha pulegium (Mp) decocts was evaluated in the organ bath isolated in increasing concentrations (non-cumulative), as positive controls were used nifedipine 5 × 10-8 M (antagonist of voltage dependent Ca2+ channels) atropine sulfate 5,5 × 10-9 M (non-selective muscarinic antagonist) and 3 x 10-3 M hyoscine N-butylbromide (selective M3 muscarinic antagonist). The amplitude and the frequency of contraction were quantified (during 10 minutes) after the administration of the decocts. Results. The decocts of Rubus roseus and Mentha pulegium decrease the contractions induced by acetylcholine (p < 0.001) and KCl (p <0.01) comparable to nifedipine (Mp: 4 and 8 mg/mL), atropine sulfate (Rr: 0.2 - 4 mg/mL, Mp: 0.8 and 1.6 mg/mL) and hyoscine N-butylbromide (Mp: 2.4 and 8 mg/mL). On the other hand, both decocts reduce the frequency of contraction induced by Ach (p <0.001) and KCl (p <0.001). Conclusions. The decocts of Rubus roseus and Mentha pulegium have an antispasmodic effect on isolated guinea pig ileum (Cavia porcellus).

Mentha pulegium crude extracts induce thiol oxidation and potentiate hemolysis when associated to t-butyl hydroperoxide in human's erythrocytes

ABSTRACT Mentha pulegium (Lamiaceae) tea has been used as a traditional medicine; however, the modulatory effect of M. pulegium extracts on damage to human erythrocytes associated to t-butyl hydroperoxide (t-BHP) exposure remains to be investigated. Accordingly, we perform this study in order to test the hypothesis that aqueous and ethanolic extracts of M. pulegium could modulate the hemolysis associated to t-BHP exposure, non-protein thiol (NPSH) oxidation and lipid peroxidation (measured as thiobarbituric acid reactive substances - TBARS) in human erythrocytes. Samples were co-incubated with t-BHP (4 mmol/L) and/or aqueous or ethanolic extracts (10-1000 mg/mL) during 120 min to further analysis. We found that both extracts, when associated to t-BHP, potentiate NPSH oxidation and hemolysis. Moreover, both extracts significantly prevents against t-BHP-induced TBARS production. A significant correlation among hemolysis and NPSH levels was found. Taking together, our data points that the association of M. pulegium extracts with t-BHP culminates in toxic effect to exposed erythrocytes, besides its protective effect against t-BHP-induced TBARS production. So, we infer that the use of this extract may exert negative effect during painful crisis in sickle cell anemia. However, more studies are still necessary to better investigate/understand the mechanism(s) involved in the toxic effect resultant from this association.

Constituintes voláteis de Mentha pulegium L. e Plectranthus amboinicus (Lour.) Spreng / Volatile constituents of Mentha pulegium L. and Plectranthus amboinicus (Lour.) Spreng

Nesse trabalho foi investigado a composição química de óleos essenciais de duas plantas medicinais cultivadas em Ilhéus, Brasil, Plectranthus amboinicus (Lour.) Spreng. (hortelã-grosso) e Mentha pulegium L. (poejo). Os óleos essenciais foram extraídos das folhas frescas por hidrodestilação e foram analisados por CG/FID e CG-EM, na primavera e no inverno. P. amboinicus forneceu, tanto na primavera como no inverno, 0,10 por cento de óleo sendo timol o componente majoritário. M. pulegium forneceu na primavera 0,20 por cento de óleo e no inverno 0,09 por cento. Os componentes majoritários dos óleos foram pulegona e trans-cariofileno; borneol, mentol e piperitona foram identificados em menores quantidades. No inverno foram observados maiores quantidades de mentol e isomentol. Acetatos de neoisomentila, de mentila e de isometila foram observados somente no inverno. Esse trabalho contribui para o conhecimento das espécies vegetais cultivadas na região do sul da Bahia.

This work investigated the chemical composition of the essential oils of two medicinal plants grown in Ilhéus Municipality, Bahia State, Brazil: Plectranthus amboinicus (Lour.) Spreng (Mexican mint) and Mentha pulegium L. (pennyroyal). The essential oils were extracted from fresh leaves by hydrodistillation and analyzed by GC-FID and GC-MS, in the spring and in the winter. In both seasons, P. amboinicus yielded 0.10 percent oil and had thymol as major component. M. pulegium yielded 0.20 percent and 0.09 percent oil in the spring and winter, respectively. The major components were pulegone and trans-caryophyllene, whereas borneol, menthol and piperitone were identified at small quantities. Higher menthol and isomenthol levels were detected in the winter. In addition, neoisomenthyl, menthyl and isomenthyl acetates were only observed in the winter. This work contributes to the knowledge of plant species grown in southern Bahia.

Estudio cualitativo del uso de plantas medicinales en forma complementaria o alternativa con el consumo de fármacos en la población rural de la ciudad de Bulnes, Región del Bío-Bío, Chile / Qualitative study of use medicinal plants in a complementary or alternative way with the use of among of rural population of the Bulnes City, Bío-Bío Region, Chile

In a rural population of Bulnes, Bio-Bio Region of Chile, it was assessed the daily consumption of medicinal plants and drugs. Through 168 interviews the consumption of 5 medicinal plants was evaluated, considering their application, used plant parts, type of consumption, preparation, age range of people consuming mainly medicinal plants, use of other pharmacological treatments, main diseases present in the population under study and the type of drugs consumed. The obtained results demonstrate that higher consumed medicinal plants were peppermint (Mentha piperita) chamomile (Matricaria recutita), rute (Ruta graveolens), oregano (Origanum vulgare) and pennyroyal (Mentha pulegium). The consumption of medicinal plants was mainly complementary. The therapeutic use of medicinal plants in the rural population of Bulnes, is mainly digestive and it is not necessarily related to those diseases with more prevalence or most commonly observed in the population. It is important to consider future studies to determine if medicinal plants are more likely used to reduce the digestive discomfort which arises as a lateral damage of pharmaceutical drugs.

En una población rural de la Comuna de Bulnes, VIII región de Chile, fue valorado el consumo de plantas medicinales y fármacos. A través de 168 encuestas fue valorado el consumo de 5 plantas medicinales, su aplicación, las partes mas usadas de las plantas, tipo de consumo, preparación, porcentaje del rango etáreo que consume mayoritariamente plantas medicinales, porcentaje de la población sin y con consumo de fármacos, el porcentaje de las enfermedades presentes en la población en estudio y de los fármacos consumidos. Se obtuvo que las plantas medicinales más consumidas fueron la menta (Mentha piperita), manzanilla (Matricaria recutita), ruda (Ruta graveolens), orégano (Origanum vulgare) y poleo (Mentha pulegium). El consumo de las plantas medicinales fue principalmente de tipo complementario. El uso terapéutico de las plantas medicinales en la población rural de Bulnes, es principalmente de tipo digestivo no relacionándose con las enfermedades de mayor presencia en la población. Dicho uso es probablemente para disminuir las molestias digestivas causadas por el consumo de fármacos. Es necesario realizar mas estudios tendientes a esclarecer este punto de importancia relevante.

Acaricidal effects of herb essential oils against Dermatophagoides farinae and D. pteronyssinus(Acari: Pyroglyphidae) and qualitative analysis of a herb Mentha pulegium(pennyroyal)

This experiment was undertaken to screen the acaricidal effects of herb essential oils (pennyroyal, ylang ylang, citronella, lemon grass, tea tree, and rosemary) at different doses (0.1, 0.05, 0.025, 0.0125, and 0.00625 microliter/cm2) and exposure times (5, 10, 20, 20, 30 and 60 min) on house dust mites Dermatophgoides farinae and D. pteronyssinus. The most effective acaricidal components of pennyroyal (Mentha pulegium) were analyzed using a gas chromatography-mass spectrometer (GC-MS). Of these essential oils, the most effective was pennyroyal, which is composed essentially of pulegone (> 99%), at a dose of 0.025 microliter/cm2, which at an exposure time of 5 min killed more than 98% of house dust mites. In the pennyroyal fumigation test, the closed method was more effective than the open method and maximum acaricidal effect was 100% at 0.025 microliter/cm2, 60 min. The results show that herb essential oils, in particular, pennyroyal was proved to have potent acaricidal activity

Evaluation of antimicrobial effects of 4 medicinal plants against salmonella typhymurium and comparision them with common antibiotics in veterinary medicine

Thyme [Zataria multiflora Bois.] Penny voyel [Mentha pulegium L.] Origanum [Origanum vulgare L.] and Fennel [Foeniculum vulgare Miller] are the Iranian traditional spice which are widely used in the country. Following the recent efforts to look for healthy remedies with antimicrobial potential, the effects of essential oils of these medicinal plants were investigated on Salmonella typhimurum. Chemical composition and antimicrobial effects of essential oils from plants were evaluated on Salmonella typhimurum and compared with five antibiotics enrofloxacin, chloramphenicle, erythromycin, oxytetracyclin and flumequin. The essential oils of the leavs and stems are obtained from each plants by hydrodistillation and clevenger and analyzed by GC/MS. The results of this study show that Thyme oil, compared to flumequin, oxytetracyclin and erythromycin is more effective [P<0.5]. Essential oil of Penny voyel is more effective than oxytetracyclin and erythromycin. Among essential oil of plants, Thymol oil with minimal inhibitory concentration [MIC] 156.5 micro g/ml and minimal bactericidal concentration [MBC] 312.5 micro g/ml had the best result against Salmonella typhimurum. Chemicial compounds: Thymol, Carvacrol and alpha -terpinen were found in Z. multiflora, M. pulegium, O. vulgar and F. vulgare by GC analyse. Considering the significant antibacterial effect of essential oil of Z. multiflora on effect on Salmonella typhimurum in invitro, it is recommened that, the therapeutic effect of this essential oil be studied in invivo

Caracterização biométrica e química da folha de Mentha pulegium x spicata (Lamiaceae) / Biometric and chemical characterization of Mentha pulegium x spicata (lamiaceae) leaves

O híbrido Mentha pulegium x spicata, também conhecido como poejo de praia, é uma planta perene, rasteira, ramosa, com caule arroxeado, folhas opostas, lanceoladas e serreadas no bordo, apresentando odor característico. O objetivo do presente trabalho foi realizar um estudo da anatomia foliar, incluindo um estudo de biometria tecidual e análise quantitativa de tricomas secretores/mm2 e de estômatos/mm2 em ambas as faces da folha, além de uma análise química do óleo essencial. Foram efetuadas lâminas permanentes para análise anatômica do limbo foliar e também lâminas da impressão foliar para a contagem do número de tricomas e estômatos. A análise química foi efetuada através da extração do óleo essencial por arraste à vapor d'água e analisado por CG-EM. Os tricomas capitados e peltados estão presentes em ambas as faces da folha, porém predominam na superfície abaxial. A análise de variância mostrou que há diferenças significativas para o número de tricomas capitados e tectores entre as superfícies adaxial e abaxial, mas não são significativas para o tricoma peltado. O óleo essencial analisado, mostra a presença do componente majoritário, trans-epóxido de piperitona, responsável por mais de 80% da composição relativa no óleo bruto

Estudio de la Menta pulegium boliviana / Study of the Bolivian Pulegium Mint

Por las indicaciones que hacen notables profesores al respecto de este vegetal. se ve pues que con el tiempo está llamado a ocupar un lugar preferente entre los remedios que emplea la terapéutica para curar varias enfermedades del estómago, razón por la cual vuelvo a repetir he creído conveniente ocuparme sobre el particular, mas tal vez lo encuentre rudimentario y deficiente, no obstante no haber omitido esfuerzo alguno para hacer lo mejor posible, teniendo siempre que lamentar que cuando uno trata de hacer cualquier trabajo de esta naturaleza, se encuentra a cada momento con dificultades dada la escasez de medios y material científico de que no disponemos en nuestro país.(AU)