Expression of MT1, 2, 3-MMP in invasive cervical cancer / 대한산부인과학회지

OBJECTIVE: Threre are several proteolytic enzymes such as Matrix Metalloproteinases (MMP), which are involved in tumor invasion and metastasis. The aim of this study was to determine the exprssion of Membranous Type MMPs (MT-MMPs) and investigate the relationship between their expression and questioned whether their expression is related to stages and other prognostic factors of cervical cancer. METHODS: The cervical and cervical cancer tissues were taken from the patients; healthy women (n=14), and the patients with cervical cancer (n=35). The protein expression of MT1, 2, 3-MMP with MMP-2 was examined using immunohistochemical staining and western blotting. MMP-2 activity was measured by zymogram. RESULTS: The expression of MT1, 2, 3-MMP was higher in cervical cancer than that of normal cervix (p0.05). But there were significant correlations between MT1-MMP, MT3-MMP and lymph node involvemen t (p<0.05). There was significant correlation between MT-MMPs and MMP-2 (p<0.05), too. CONCLUSION: According to the results, MT-MMP expression could be associated with the pathogenesis of cervical cancer. In addition, the evaluation fo MT-MMPs expression might be helpful to predict lymph node metastasis in cervical cancer. Further prospective study with a large number of cases is needed in future.

Expression of mRNA for membrane-type 1, 2, and 3 matrix metalloproteinases in human laryngeal cancer / 中国医学科学杂志(英文版)

<p><b>OBJECTIVE</b>To investigate correlation of expressions of membrane-type 1, 2, and 3 matrix metalloproteinases (MT1, MT2, and MT3-MMP) to the invasion and metastases in laryngeal cancer.</p><p><b>METHODS</b>Reverse transcription-polymerase chain reaction (RT-PCR) was used to examine the mRNA level of MT1, MT2, and MT3-MMP in 24 patients with laryngeal cancer. The relationships of these three MT-MMP expressions to clinicopathology were analyzed by statistics.</p><p><b>RESULTS</b>The expressions of MT1, MT2, and MT3-MMP were significantly higher in laryngeal cancer tissues than those in para-tumorous tissues (P < 0.01) and had a close relationship with invasive depth (P < 0.05). But no significantly different expressions of these three MT-MMPs were found in different primary location and different histological grade of laryngeal cancer (P > 0.05). The expression of MT1-MMP was obviously higher in patients with metastatic lymph nodes than that in patients without metastatic lymph nodes (P < 0.05).</p><p><b>CONCLUSION</b>MT1, MT2, and MT3-MMP play an important role in the progression of laryngeal cancer, and MT1-MMP may serve as a reliable marker in estimating invasive and metastatic potency of laryngeal cancer. Suppressing expressions of MT1, MT2, and MT3-MMP early may inhibit the invasion and metastases of laryngeal cancer.</p>