RESUMO
Por largo tiempo la patogénesis del mixedema pretibial fue un misterio. Investigaciones recientes han corroborado la naturaleza autoinmune del padecimiento y han señalado el papel importante de citoquinas en la expresión de moléculas HLA-DR en su fisiopatología. Como se sospechaba, el fibroblasto es la célula blanco efectora
Assuntos
Fibroblastos/citologia , Fibroblastos/metabolismo , Glicosaminoglicanos , Glicosaminoglicanos/metabolismo , Dermatoses da Perna/patologia , Mixedema/imunologia , Mixedema/patologiaRESUMO
This study was performed to prove the hypothesis that oncogene expressions would have the same patterns with those of cellular growth to growth factors in FRTL-5 cells. Ribonucleic acids of FRTL-5 were extracted at 15', 30', 60' and 120' after administration of growth factors to quiescent FRTL-5, and blotted to the nitrocellulose membrane. They were hybridized with radiolabelled c-fos, c-myc and beta-actin probes. Hybridized dot blots were autoradiographed and the amount of radioactivity was measured by densitometry. Densitometric readings were used as the indices of oncogene expressions. Expressions of c-fos and c-myc were more prominent in combined administrations of TSH (10 mU/ml) and IGF-I (100 ng/ml) or IgG of Graves' disease (Graves' IgG; 1 mg/ml) and IGF-I than in combined administration of TSH and Graves' IgG. IgG of primary myxedema suppressed oncogene expressions by TSH or Graves' IgG, but not by IGF-I. From the above results, it was suggested that expressions of c-fos and c-myc to growth factors would have similar patterns with those of cell growth to growth factors in FRTL-5, and the actions of TSH and Graves' IgG would be manifested through same signal transduction system, but IGF-I would be manifested by its own.
Assuntos
Ratos , Animais , Divisão Celular/efeitos dos fármacos , Linhagem Celular/citologia , Expressão Gênica/efeitos dos fármacos , Doença de Graves/imunologia , Substâncias de Crescimento/genética , Imunoglobulina G/farmacologia , Fator de Crescimento Insulin-Like I/farmacologia , Mixedema/imunologia , Proteínas Proto-Oncogênicas c-fos/genética , Proteínas Proto-Oncogênicas c-myc/genética , RNA/análise , Ratos Endogâmicos F344 , Glândula Tireoide/citologia , Tireotropina/farmacologia , Fatores de TempoRESUMO
A national survey was carried out in 1987 to assess the prevalence of rheumatic fever and chronic rheumatic heart disease among Schoolchildren aged between 6 and 1 5 years in Saudi Arabia. A sample size of 47810 [2% of the target population] was subjected to a clinical and laboratory evaluation. The study revealed the prevalence of rheumatic fever to be 0.3 per 1000 and the prevalence of chronic rheumatic heart disease 2.8 per 1000, giving an overall rate of 3.1 per 1000 Schoolchildren [6-15 years]. The prevalence was higher in rural areas and at low socioeconomic levels