RESUMO
A total of 75 clinically diagnosed and radiologically evident cases suggestive of pulmonary tuberculosis were selected for study. Sputum sample of each patient was screened for AFB by Ziehl Neelsen staining and culture. On examination 20 smears were found positive for AFB and 55 smears were negative by concentration method. A total of 23 samples were found to be culture positive and 52 were culture negative. Of these, 22 stains were identified as Mycobacterium tuberculosis, one was identified as M. Scrofulaceum. Of the 75 patients 3 were seropositive for HIV-I antibodies. Out of these 3, one was found to be smear and culture positive and was identified as M. scrofulaceum. Other two seropositive patients were smear and culture negative for AFB.
Assuntos
Infecções Oportunistas Relacionadas com a AIDS/microbiologia , Soropositividade para HIV/complicações , HIV-1 , Humanos , Índia , Mycobacterium/isolamento & purificação , Mycobacterium scrofulaceum/isolamento & purificação , Mycobacterium tuberculosis/isolamento & purificação , Tuberculose Pulmonar/complicaçõesRESUMO
Cultivation trials for Mycobacterium leprae resulted in growth of Mycobacterium psychrophilum (L). Media were inoculated with host grown Mycobacterium leprae cells from armadillo tissues, Nu mice foot pads or human lepromata. Cultures were obtained in liquid and on semisolid multifactoria 1 media containing water soluble palmitic acid or its salts. Ammonium thioglycolate and Napalmitate served as carbon and energy sources. The water soluble palmitic acid remained in perfect solution following sterilization in the autoclave, thus easily accessible to the cells. The cyclodextrin-Fe complex served as a siderophore to grow the obtained leprosy derived psychrophilic cells. The leprosy derived cultures and subcultures grew opimally at+10 degrees Celssius but deteriorated rapidly at + 32 degrees Celsius, in the multifactorial media. No growth occurred in 7H9 media. Cultures were not identified for classification.
Assuntos
Humanos , Animais , Camundongos , Hanseníase/microbiologia , Mycobacterium leprae/isolamento & purificação , Mycobacterium/crescimento & desenvolvimento , Ácidos Palmíticos , Meios de Cultura , Hanseníase Tuberculoide/microbiologia , Hanseníase Virchowiana/microbiologia , Mycobacterium avium/crescimento & desenvolvimento , Mycobacterium avium/isolamento & purificação , Mycobacterium leprae/crescimento & desenvolvimento , Mycobacterium phlei/crescimento & desenvolvimento , Mycobacterium phlei/isolamento & purificação , Mycobacterium scrofulaceum/crescimento & desenvolvimento , Mycobacterium scrofulaceum/isolamento & purificação , Mycobacterium/isolamento & purificaçãoRESUMO
Se reporte 1 caso de 1 paciente de 71 años que presentó trastornos respiratorios por más de 11 años. La microscopia directa fue negativa en 4 esputos y finalmente el cultivo permitió en 5 ocasiones una cepa caracterizada bioquímicamente como mycobacterium scrofulaceum. El caso se correspondió con una micobacteriosis