1.
Indian J Exp Biol
;
2001 Jul; 39(7): 650-3
Artigo
em Inglês
| IMSEAR
| ID: sea-57161
RESUMO
An heterologous transformation system for entomopathogenic fungi B. bassiana and M. anisopliae was developed based on the use of A. nidulans nitrate reductase gene (niaD). B. bassiana and M. anisopliae niaD stable mutants were selected by treatment of protoplast with ethane methane sulphonate (EMS) and regenerated on chlorate medium. The cloned gene was capable of transforming B. bassiana and M. anisopliae at a frequency of 5.8 to 20 transformants per microg of DNA. Most of them were mitotically stable.
Assuntos
Aspergillus niger/enzimologia , Genes Fúngicos , Hypocreales/citologia , Mitose , Mutação , Nitrato Redutase , Nitrato Redutases/genética , Transformação Genética
2.
Indian J Exp Biol
;
1980 Oct; 18(10): 1165-7
Artigo
em Inglês
| IMSEAR
| ID: sea-58744