Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Adicionar filtros








Intervalo de ano
1.
Acta cir. bras ; 32(3): 219-228, Mar. 2017. tab, graf
Artigo em Inglês | LILACS | ID: biblio-837687

RESUMO

Abstract: Purpose: To investigate the microbiological, inflammatory and oxidant effects of adjuvant ozone administration in experimental rat vascular graft infection model which has not been previously investigated. Methods: Forty adult Wistar rats were divided into Sham, Control, Vancomycin, Ozone, Vancomycin+Ozone groups. Grafts were inoculated with Methicillin-resistant Staphylococcus aureus (MRSA) strain and implanted subcutaneously. Rats were treated intraperitoneally with ozone and /or intramuscularly with vancomycin for 10 days. Grafts were evaluated by quantitative bacterial cultures. Blood samples were harvested for determination of thiol-disulphide and cytokine profiles. Results: There was no significant difference in bacterial counts between Control and Ozone Groups. In the Ozone Group median colony count was significantly higher than the Vancomycin and Vancomycin+Ozone Groups. Total thiol and disulphide levels increased and disulphide/native thiol and disulphide/total thiol ratios decreased in Ozone Group significantly. Albumin levels decreased significantly in Vancomycin and Vancomycin+Ozone Groups compared to the Sham Group. IL-1 and TNF-alpha levels significantly increased in infected rats. Decreased levels of VEGF due to infection reversed by ozone therapy in control and vancomycin groups. Conclusions: We didn't observe any benefit of the agent on MRSA elimination in our model. Likewise, effects of ozone on thiol-disulphide homeostasis and inflammatory cytokines were contradictory.


Assuntos
Animais , Masculino , Oxidantes Fotoquímicos/farmacologia , Ozônio/farmacologia , Infecções Estafilocócicas/tratamento farmacológico , Dissulfetos/sangue , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Enxerto Vascular , Valores de Referência , Fatores de Tempo , Doenças Vasculares/microbiologia , Albumina Sérica/análise , Vancomicina/farmacologia , Contagem de Colônia Microbiana , Distribuição Aleatória , Reprodutibilidade dos Testes , Citocinas/sangue , Resultado do Tratamento , Ratos Wistar , Transplantes/microbiologia , Staphylococcus aureus Resistente à Meticilina/crescimento & desenvolvimento , Homeostase/efeitos dos fármacos , Antibacterianos/farmacologia
2.
J. appl. oral sci ; 21(2): 177-182, Mar-Apr/2013. graf
Artigo em Inglês | LILACS | ID: lil-674363

RESUMO

Ozone is an important disinfecting agent, however its influence on enamel adhesion has not yet been clarified. Objective: Evaluate the influence of ozone pretreatment on the shear strength of an etch-and-rinse and a self-etch system to enamel and analyze the respective failure modes. Material and Methods: Sixty sound bovine incisors were used. Specimens were randomly assigned to four experimental groups (n=15): Group G1 (Excite® with ozone) and group G3 (AdheSE® with ozone) were prepared with ozone gas from the HealOzone unit (Kavo®) for 20 s prior to adhesion, and groups G2 (Excite®) and G4 (AdheSE®) were used as control. Teeth were bisected and polished to simulate a smear layer just before the application of the adhesive systems. The adhesives were applied according to the manufacturer's instructions to a standardized 3 mm diameter surface, and a composite (Synergy D6, Coltene Whaledent) cylinder with 2 mm increments was build. Specimens were stored in 100% humidity for 24 h at 37°C and then subjected to a thermal cycling regimen of 500 cycles. Shear bond tests were performed with a Watanabe device in a universal testing machine at 5 mm/min. The failure mode was analyzed under scanning electron microscope. Means and standard deviation of shear bond strength (SBS) were calculated and difference between the groups was analyzed using ANOVA, Kolmogorov-Smirnov, Levene and Bonferroni. Chi-squared statistical tests were used to evaluate the failure modes. Results: Mean bond strength values and failure modes were as follows: G1- 26.85±6.18 MPa (33.3% of adhesive cohesive failure); G2 - 27.95±5.58 MPa (53.8% of adhesive failures between enamel and adhesive); G3 - 15.0±3.84 MPa (77.8% of adhesive failures between enamel and adhesive) and G4 - 13.1±3.68 MPa (36.4% of adhesive failures between enamel and adhesive). Conclusions: Shear bond strength values of both adhesives tested on enamel were not influenced by the previous application of ozone gas.


Assuntos
Animais , Bovinos , Resinas Acrílicas/química , Colagem Dentária/métodos , Esmalte Dentário/efeitos dos fármacos , Metacrilatos/química , Oxidantes Fotoquímicos/farmacologia , Ozônio/farmacologia , Resistência ao Cisalhamento , Resinas Compostas/química , Falha de Restauração Dentária , Esmalte Dentário/química , Adesivos Dentinários/química , Teste de Materiais , Microscopia Eletrônica de Varredura , Ácidos Fosfóricos/química , Distribuição Aleatória , Propriedades de Superfície
3.
Braz. oral res ; 26(2): 126-131, Mar.-Apr. 2012. ilus, tab
Artigo em Inglês | LILACS | ID: lil-622908

RESUMO

Ozone is a known oxidant present in the atmosphere and is commercially produced by simple ozonizer machines. It is a powerful antimicrobial agent in its gaseous and aqueous forms. Ozone readily dissolves in water and retains its antimicrobial property even in the dissolved state. In this study, the effect of 0.1 ppm ozonated water was analyzed on 24-hour supragingival plaque (SP) samples in situ. SP was collected from the two most posterior teeth in the contra-lateral quadrants before and after a 30-second rinse with either distilled water (control group) or 0.1 ppm ozonated water (test group). The plaque was used to count the number of total bacteria, total anaerobic bacteria, Streptococcus mutans, and Candida albicans on selective agar media. The statistical analysis of the number of colony forming units (CFUs) obtained demonstrated a significant antimicrobial effect of ozonated water on the total bacteria (p = 0.01) and anaerobes (p = 0.02). A reduction in the post-rinse CFU count for Streptococcus mutans was also observed, but the effect was not statistically significant (p = 0.07). The Candida species was only grown from one sample. Ozonated water at the 0.1 ppm concentration was effective in reducing the load of 24-hour plaque bacteria, but it did not eliminate them completely.


Assuntos
Adolescente , Adulto , Humanos , Adulto Jovem , Anti-Infecciosos/farmacologia , Candida albicans/efeitos dos fármacos , Placa Dentária/microbiologia , Oxidantes Fotoquímicos/farmacologia , Ozônio/farmacologia , Streptococcus mutans/efeitos dos fármacos , Água/farmacologia , Análise de Variância , Contagem de Colônia Microbiana , Fatores de Tempo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA