RESUMO
Squamous cell carcinoma of the cervix (SCCC) is one of the leading causes of death in developing countries. Infection with high-risk human papillomavirus (HPV) is the major risk factor to develop malignant lesions in the cervix. Polymorphisms of the MHC and p53 genes seem to influence the outcome of HPV infection and progression to SCCC, although controversial data have been reported. MHC are highly polymorphic genes that encode molecules involved in antigen presentation, playing a key role in immune regulation, while p53 is a tumor suppressor gene that regulates cell proliferation. The HPV E6 protein from high-risk types binds p53 and mediates its degradation by the ubiquitin pathway. The role of these polymorphisms in genetic susceptibility to HPV infection and to SCCC remains under investigation
Assuntos
Humanos , Feminino , Carcinoma de Células Escamosas/genética , Papillomavirus de Coelho Cottontail , Genes p53 , Papillomaviridae/genética , Infecções por Papillomavirus/genética , Polimorfismo Genético , Infecções Tumorais por Vírus/genética , Neoplasias do Colo do Útero/genética , Carcinoma de Células Escamosas/virologia , Predisposição Genética para Doença , Infecções por Papillomavirus/virologia , Fatores de Risco , Infecções Tumorais por Vírus/virologia , Neoplasias do Colo do Útero/virologiaRESUMO
Los virus del papilloma humano estan asociados estrechamente con el cancer de cuello de utero. Se ha demostrado que los virus 6 y 11 estan asociados con las lesiones premalignas, mientras que los 16 y 18 a las lesiones malignas del cancer cervical. En el presente trabajo se describe la obtencion de un anticuerpo monoclonal murino contra el virus 16 del pailloma humano, utilizando el metodo convencional de fusion somatica, el cual reconoce por inmunohistoquimica sobre cortes de tejido de biopsias de cervix uterino las celulas coilociticas y disqueratosicas ubicadas en las capas mas superficiales del epitelio infectado. La presencia del virus tipo 16 de las muestras analizadas se comprobo por estudios de hibridacion in situ realizadas con anterioridad. El uso de este anticuerpo monoclonal en Anatomia Patologica permitira el diagnostico de la presencia del virus 16 del papiloma humano en aquellas muestras que presenten las atipias celulares caracteristicas de esta enfermedad
Assuntos
Animais , Camundongos , Anticorpos Monoclonais , Anticorpos Monoclonais/isolamento & purificação , Fusão Celular , Papillomavirus de Coelho Cottontail/isolamento & purificação , Imuno-Histoquímica/métodos , Neoplasias do Colo do Útero/diagnósticoAssuntos
Humanos , Animais , Masculino , Feminino , Adulto , Condiloma Acuminado/etiologia , Infecções Tumorais por Vírus/genética , Papillomaviridae/genética , Neoplasias Penianas/etiologia , Neoplasias do Colo do Útero/etiologia , Animais , Papillomavirus de Coelho Cottontail , Epidermodisplasia Verruciforme/etiologia , Vírus Oncogênicos , Simplexvirus/imunologia , Neoplasias do Colo do ÚteroRESUMO
In 1933 Shope described an infectious papilloma of wild cattontail rabbits. Shope rabbit papilloma is a virus-induced epidermal neopIasm indigenous to western cottontail rabbits and readily transmissible to domestic rabbits by inocuiation with an cell-free extract of wild cottontail papilloma. Shope papilloma induced in the domestic rabbits may spoataneously, remain benign, or progress to carcinoma. Kidd et al. (1936) noted that the regression is not correlated with the titer of virus neutralizing antibody. The possibility that papilloma regression is brought about by a mechanism anaIogous to that operating in allograft rejection was suggested by kidd et al. (1938) with the view of the histological resemblance between papilloma regression and rejection of allografted tissue. That regression is mediated by an immune mechanism is strongly indicated by the findings of Evans et al.(1962) that the proportion of rabbits showing regression is substantially increased by vaccination of rabbits with their own papilloma tissue or with papilloma tissue from another rabbits. McMichael (1967) demonstrated that suppression of regression was achieved by use of large doses of corticosteroid. The effect of 5-fluorouracil on human warts was mentioned as being effective by Goldman et al. (1963). In Korea resinous extract from Paulownia coreana has long been used empirically in the treatment of human warts. In this investigation effects of dexamethasone administration on the development and the spontaneous regression of Shope papillomas in domestic rabbits were studied. At the same time, observations were made to evaluate the comparative effectiveness of topical application of 5% 5-fluorouracil ointment and resinous extract from Paulownia coreana on Shope papilloma in the domestic rabbit. The results are summarized as follows: l. Administration of dexamethasone significantly reduced the spontaneous regression of Shope papillomas in domestic rabbits by 12 weeks after virus inoculation. 2. Topical application of 5% 5-fluorouracil ointment on Shope papillomas cured the tumors by 4 weeks of drug application. However, similar treament with resinous extract from Paulownia coreana was without any demonstrable effects. 3. Marked losses of body weights became manifest in dexamethasone treated rabbits. 4. Reinoculation of Shope papilloma virus into 2 rabbits whose papillomas had regressed 3 months ago failed to produce papillomas.