RESUMO
In this study, we evaluated the effect of low and high molecular weight polycyclic aromatic hydrocarbons (PAHs),
Assuntos
Aspergillus/crescimento & desenvolvimento , Benzo(a)pireno/farmacologia , Micélio/efeitos dos fármacos , Fenantrenos/farmacologia , Hidrocarbonetos Policíclicos Aromáticos/farmacologia , Pirenos/farmacologia , Esporos Fúngicos/efeitos dos fármacos , Trichoderma/crescimento & desenvolvimento , Aspergillus/efeitos dos fármacos , Aspergillus/metabolismo , Biodegradação Ambiental , Hidrocarbonetos Policíclicos Aromáticos/metabolismo , Microbiologia do Solo , Poluentes do Solo , Trichoderma/efeitos dos fármacos , Trichoderma/metabolismoRESUMO
The in vitro activity of four 2-nitropropene derivatives, 1-(3-benzothienyl)-2-nitropropene (N1), 1-(3-thienyl)-2-nitropropene (N2), 1-(5-bromo-2-thienyl)-2-nitropropene (N3) and 1-(4-bromo-2-thienyl)-2-nitropropene (N4), were tested against cultures of the parasite Trypanosoma cruzi. Cytotoxicity studies were performed using Vero cells. The blood trypomastigotes, amastigotes and epimastigotes showed differential degrees of sensitivity towards the four tested compounds; the highest activity against the epimastigotes and blood tripomastigotes was exhibited by N1, followed by N3, N4 and finally N2. In contrast, whereas the compounds N1, N3 and N4 exerted similar magnitudes of activity against amastigotes, N2 was found to be a much less potent compound. According to our results, the compound N1 had the highest level of activity (IC50: 0.6 ìM) against epimastigotes.
Assuntos
Animais , Antiprotozoários/farmacologia , Nitrocompostos/farmacologia , Trypanosoma cruzi/efeitos dos fármacos , Análise de Variância , Chlorocebus aethiops , Pirenos/farmacologia , Trypanosoma cruzi/crescimento & desenvolvimento , Células VeroRESUMO
Administration of pyrene-linked fatty acids and lipids to cultured cells or an enveloped (vesicular stomatitis) virus induced photosensitization which, following irradiation with a long ultra-violet light (LUV), resulted in killing of the cells and loss of the infectivity of the virus with the following specific effects. (i) LUV illumination of the pyrene-sphingomyelin administered cultured skin fibroblasts derived from normal individuals and patients with Niemann-Pick disease permitted selective killing of the latter. (ii) Similarly LUV illumination of pyrenedodecanoic acid (P12) incubates of leukemic cell lines mixed with human bone marrow cells permitted selective killing of the former. (iii) LUV illumination of P12 incubates of vesicular stomatitis virus decreased the infectivity of the virus by up to 12 logs.