Pyridoxine effect on the antidepressant action of imipramine in albino mice

To evaluate the behavioral effect of pyridoxine on the antidepressant action of imipramine. Male Wistar albino mice of weights 25-35gms were used. Two experiments were carried out; the first on the acute effect of pyridoxine on the duration of immobility, and the second on the sub-chronic effects of pyridoxine alone and in combination with imipramine. In the first experiment, 4 groups of animals received saline, 65, 125, and 250mg/kg pyridoxine. Forced swimming test [FST] was performed 30 minutes after drug administration. In the second experiment, 6 groups of mice were used. The first group received saline, the second group received imipramine 10 mg/kg, the third group received pyridoxine 65mg/kg, the fourth group received pyridoxine 250mg/kg, the fifth group received combined treatment of imipramine and pyridoxine 65 mg/kg, while the sixth group received a combined treatment of imipramine and pyridoxine 250 mg/kg. Administration of drugs was at 24, 5, and one hour before the test. This work was carried out in the Biotechnology Research Center, Twisha, Libya, in June 2007. Acute administration of pyridoxine did not change the duration of immobility compared to the control group. Sub-chronic administration showed that pyridoxine [65mg/kg] did not change the immobility time, while a higher dose of pyridoxine [250mg/kg] decreased the immobility time. Imipramine at 10mg/kg reduces the immobility time significantly. Pyridoxine did not change imipramine action. Pyridoxine alone may produce an antidepressant effect. Pyridoxine in combination with imipramine did not change the imipramine action

Piridoxina en el manejo de disquinesias tardías: un estudio placebo controlado, randomizado, doble ciego y cruzado / Pyridoxine for drug induced dyskinesia: a placebo-controlled randomized cross-over trial

Las Disquinesias tardías son reacciones adversas frecuentes e invalidantes de aquellos fármacos relacionados con la transmisión dopaminérgica. Una proporción importante de pacientes que la padecen no responden a las terapias actualmente vigentes. En este estudio se randomizó a 28 pacientes portadores de Disquinesias tardías severas y refractarias a tratamiento habitual, provenientes del Instituto Psiquiátrico de Santiago, en dos grupos que recibieron Piridoxina (500 mg al día) o placebo por 4 semanas, siguiendo un periodo de lavado de 7 días tras los cuales ambos grupos se cruzaron, manteniendo tratamiento por 4 semanas adicionales. Se utilizó la escala AIMS (Abnormal Involuntary Movement Scale) para evaluar a cada paciente en la semana 2 y 4 de cada etapa del estudio. La Piridoxina fue bien tolerada y no hubo efectos adversos en el periodo de estudio, no encontrándose diferencias significativas en la mejoría de Disquinesias Tardía entre los grupos que recibieron Piridoxina o Placebo.

Tardive Dyskinesia is a common and disabling adverse effect of drugs acting on Dopaminergic pathways. An important proportion of patients does not respond to the conventional treatment. In this study 28 severe and refractory patients from a Psychiatric Hospital were randomized in a cross over design to placebo and high doses (500 mg per day) of Pyridoxine for 4 weeks each one. The patients were evaluated using the Abnormal Involuntary Movement Scale (AIMS) at 2 and 4 weeks of each cycle. Pyridoxine was well tolerated, and no adverse effect occurred during the study. No statistical differences between Pyridoxine and Placebo were found. Surprisingly, in both groups equally good responses were found.

Germination and growth of dature innoxia mill i in vitro studies on the effect of pre-sowing seed treatment with selected vitamins on germination and seedling growth

An inexpensive technique for ensuring high percentage of in vitro seed germination of Datura innoxia Mill. a hard to germinate medicinal plant producing scopolamine, was tested successfully in a factorial randomised experiment. Assay of seeds for their native vitamin B1, B6 and C contents revealed very low concentrations. They were, therefore, treated for 18 h at 20°C with 0, 250, 500, 750 or 1,000 ppm of these three vitamins as well as B-complex and incubated in petri dishes for 5 weeks. Counts, made at weekly intervals, indicated 28 days as the optimal stage for germination. Growth parameters, studied at 35 days were found to be significantly affected by all treatments at P=0.05. Out of the four vitamins treated, B6 and, among the concentrations, 500 ppm alone and in combination proved most efficacious in breaking seed dormancy and accelerating early seedling growth of D. innoxia

Effect of vitamin B6 on lenses of diabetic rats.

Vitamin B6 is essential for the metabolism of fat, carbohydrate and protein. In this study the effect of vitamin B6 on diabetes induced impairments in rat lenses was investigated. Although macroscopic examination revealed no opacification of rat lenses in any groups, uncontrolled induced diabetes caused significant decreases in lens glutathione and increases in lens protein nonenzymatic glycosylation and blood glucose. Administration of vitamin B6 did not inhibit these diabetes induced alterations significantly. SDS-polyacrylamide gel electrophoresis revealed some significant differences in some protein bands between groups.

possible alteration in some lipid metabolic aspects in rats during vitamin C and B6 monotherapy

The effect of vitamins C and B6 monotherapy on some lipid metabolic aspects was studied in normal and alloxan diabetic rats. Long-term administration of vitamin C [30 mg/kg/day] and vitamin B6 [14 mg/kg/ day] induced significant reduction in serum total cholesterol triglycerides and NEFA levels, serum LDL-cholesterol levels decreased significantly after vitamin C treatment while, the change during vitamin B6 therapy is up and down. Serum HDL-cholesterol was markedly increased while, VLDL-cholesterol was mostly nonsignificantly changed following the administration of the two vitamins. Furthermore, vitamin C and B6 supplementation reduced lipid peroxidation in normal and diabetic groups. The results suggested that, the therapeutic doses of vitamins C and B6 long-term therapy increase LDL metabolism and produces hypocholesterolemia in male rats. On the other hand, vitamins C and B6 has a beneficial effect on cardiovascular risk profile

Vitamins prevention of diabetic complication: effect of vitamins C and B6 on glycosylated hemoglobin and sorbitol dehydrogenase

The experiments were undertaken to assess the role of vitamin C [ascorbic acid] and vitamin B6 [pyridoxine] in relation to sorbitol dehydrogenase activity and glycosylated hemoglobin levels in sorbitol pathway linked neural and vascular dysfunction and nonenzymatic glycosylation in rats with alloxan induced diabetes. Vitamins C and B6 monotherapy were given orally to control and diabetic rats. After 30 days and 45 days of treatment, glycosylated hemoglobin levels were decreased significantly [p <0.05 to 0.001]. Sorbitol dehydrogenase activity in normal rats during vitamin C therapy were increased significantly [p <0.08 to 0.001] from 7 to 9 folds after 30 days and 45 days, respectively. In diabetic rats sorbitol dehydrogenase activity elevated about 2.5 folds. Vitamin B6 increased sorbitol dehydrogenase activity about 7 folds in normal and 3 to 2.5 folds after 30 days and 45 days, respectively, in diabetic rats. These observations together with other evidence, suggested the significant relationship between glycosylated hemoglobin, sorbitol dehydrogenase and intake of vitamin C and vitamin B6. The elevated sorbitol dehydrogenase activity associated with the previously reported sorbitol levels. These vitamins supplementation is effective in individuals with insulin-dependent diabetes mellitus [IDDM]. They may be potentially important in controlling glucose-induced nonenzymatic glycosylation of proteins and, therefore may be a preferable drugs for inhibiting glucose induced nonenzymatic glycosylation, neural and vascular dysfunction

Effect of steroid hormones and vitamin B6 on age dependent changes in aminotransferases in rat.

Experiments have been carried out on liver and Kidneys to study the age dependent changes in alanine and aspartate aminotransferases and the influence of steroid hormones corticosterone (catabolic), testosterone (anabolic) and vitamin B6 on these changes. The rats used were of the ages between 7 to 73 weeks. It is observed that specific activity of alanine aminotransferase as well as the activity per liver increased with age. The same is true with the kidneys. Corticosterone treatment brings about two and half fold increase in activity in the liver of younger rats, whereas there is only 25% increase in the oldest group. Testosterone and vitamin B6 lower this activity, the latter showed more pronounced effect. In the case of kidneys the changes are marginal. Aspartate aminotransferase shows marginal changes in the specific activities in both liver and kidney, whereas the total activity increases with age, except in the case of liver where there is decrease at 73 weeks. Both testosterone and vitamin B6 have marginal influence on the kidney enzyme. There is no apparent explanation for the differential behaviour of the two enzymes.

Control neuroendocrino de la lactancia / Neuroendocrine control of lactation

Se revisan los principales mecanismos neuroendocrinos y hormonales que participan en la preparación de la glándula mamaria para la lactancia fisiológica, así como la participación de estos mecanismos en la síntesis, secreción y expulsión de la leche durante el amamantamiento. Asimismo, se analisan y comentan los resultados obtenidos con el uso terapéutico de las hormonas sexuales, el clomifeno, la piridoxina, el nomifensín, la ergonovina y la bromocriptina para inhibir o suprimir la secreción láctea en el periodo posparto, y la utilidad terapéutica de la hormona hipotalámica liberadora de tirotropina (TRH) y de la metoclopramida para estimular la lactancia en el puerperio