Isolamento do vírus da rinotraqueíte infecciosa bovina do sêmen congelado para comercializaçäo / Isolation of infectious bovine rhinotracheitis virus from semen samples of a Brazilian AI study

Bovine herpesvirus 1 (HVB1) was isolated in vitro using two MDBK cells lines from Brazilian commercial semen. The identification of the virus was carried out by direct fluorescent antibody test in cultures showing cytophatic effect