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1.
Journal of Huazhong University of Science and Technology (Medical Sciences) ; (6): 144-6, 2008.
Artigo em Inglês | WPRIM | ID: wpr-634590

RESUMO

In order to investigate the role of Twist gene in the metastasis of hepatocellular carcinoma (HCC), total RNA was respectively extracted from three HCC cell strains with different metastatic potentials, HepG2, MHCC-97L and MHCC-97H. The first strand cDNA was synthesized by reverse transcription, which was then used as template to perform fluorescent quantitative polymerase chain reaction (FQ-PCR). The quantity of Twist gene expression was normalized by that of the housekeeping gene, GAPDH for each sample. ANOVA was used to estimate the relationship between Twist gene and metastasis potential of HCC. The results showed that the normalized initial cDNA concentrations of Twist gene in HepG2, MHCC-97L and MHCC-97H were (9.45+/-0.25)x10(-4), (1.82+/-0.41)x10(-3), (3.06+/-0.62)x10(-3), respectively. FQ-PCR revealed significant differences in the expression level of Twist among HCC cell strains with different metastatic potentials. It was concluded that high expression level of Twist was closely associated with more aggressive behaviors of HCC. Twist provides a novel indicator for HCC metastasis.


Assuntos
Carcinoma Hepatocelular/metabolismo , Linhagem Celular Tumoral , DNA Complementar/metabolismo , Relação Dose-Resposta a Droga , Regulação Neoplásica da Expressão Gênica , Neoplasias Hepáticas/metabolismo , Metástase Neoplásica , Proteínas Nucleares/biossíntese , Reação em Cadeia da Polimerase , RNA/metabolismo , Proteína 1 Relacionada a Twist/biossíntese
2.
Journal of Korean Medical Science ; : 50-55, 2005.
Artigo em Inglês | WPRIM | ID: wpr-110323

RESUMO

Pleomorphic carcinoma of the lung (PCL) is characterized by a mixture of sarcomatoid and carcinoma components, and a poor prognosis. However, no immunophenotype of tumor markers has been characterized in PCL. To charaterize the immunophenotype for CD99 in PCL, we performed an immunohistochemical evaluation of PCLs for thyroid transcription factor-1 (TTF-1), cytokeratin (CK) 7 and 20, and for CD99. CD99 was found to be expressed in both carcinomatous (47%) and sarcomatous components such as spindle cells (92%) and giant cells (57%). In the case of spindle cells, CK7 was expressed in 6 cases (46%) and TTF-1 in 2 cases (15%), whereas for giant cells CK7 was expressed in 8 cases (57%) and TTF-1 in one case (7%). However, CK20 was not expressed in either the carcinomatous or sarcomatous components in any case. Thus, CD99 was found to be widely expressed in both sarcomatous and carcinoma component in PCL. A clinicopathological analysis showed no direct correlation between the expression of CD99 and the clinical indices (stage, survival rate, invasion) of PCL.


Assuntos
Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Antígenos CD/biossíntese , Carcinoma/metabolismo , Moléculas de Adesão Celular/biossíntese , Imuno-Histoquímica , Imunofenotipagem , Proteínas de Filamentos Intermediários/biossíntese , Queratinas/biossíntese , Neoplasias Pulmonares/metabolismo , Proteínas Nucleares/biossíntese , Prognóstico , Sarcoma/metabolismo , Fatores de Tempo , Fatores de Transcrição/biossíntese
3.
Journal of Korean Medical Science ; : 494-500, 2003.
Artigo em Inglês | WPRIM | ID: wpr-156013

RESUMO

This study was aimed to evaluate the prevalence and prognostic implication of thyroid transcription factor-1 (TTF-1) immunoreactivity in 81 human lung carcinomas, including 65 cases of non-small cell lung carcinoma (NSCLC) and 16 cases of small cell lung carcinoma (SCLC); and also to investigate its relationship with the cell proliferation and regulation by immunostaining of Ki-67 and p53 proteins, respectively. The immunohistochemical staining for TTF-1(clone 8G7G3/1) was performed and several clinicopathologic variables and the follow-up data were obtained. The immuno-staining results for TTF-1 were semiquantitatively interpreted as negative and positive. Of NSCLCs, TTF-1 is highly expressed in adenocarcinomas (76%), whereas squamous cell carcinomas revealed no immunoreactivity (0%). SCLCs showed strong TTF-1 expression (88%). In NSCLC, TTF-1 expression was inversely correlated with Ki-67 proliferative activity and independent of p53 overexpression. TTF-1(+) group tended to show better survival than TTF-1(-) group in NSCLC. Conclusively, these observations suggest that TTF-1 is a sensitive and specific diagnostic marker for pulmonary adenocarcinomas and SCLCs; that TTF-1 might have a good prognostic implication based on its inverse correlation with Ki-67 proliferative activity and tendency for better survival in NSCLC; that this cell lineage marker may play a role in the molecular pathogenesis of lung cancers at the level of transcription.


Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Adenocarcinoma/diagnóstico , Carcinoma Pulmonar de Células não Pequenas/diagnóstico , Carcinoma de Células Pequenas/diagnóstico , Divisão Celular , Linhagem Celular Tumoral , Linhagem da Célula , Imuno-Histoquímica , Antígeno Ki-67/biossíntese , Neoplasias Pulmonares/diagnóstico , Proteínas Nucleares/biossíntese , Prognóstico , Proteína Supressora de Tumor p53/biossíntese , Sensibilidade e Especificidade , Fatores de Tempo , Fatores de Transcrição/biossíntese , Transcrição Gênica , Biomarcadores Tumorais
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