Immunohistochemical identification of plasma protein deposits in the wall of lenticulostriate arteries in patients with long-standing hypertension, with and without lipohyalinosis / Identificação imuno-histoquímica de depósitos de proteínas plasmáticas na parede das artérias lentículo-estriadas em pacientes com hipertensão arterial de longa duração, com e sem lipo-hialinose

PURPOSE: To investigate, through an immunohistochemical method, whether there is deposition of plasma proteins in the wall of lenticulostriate, cortical and leptomeningeal arteries of hypertensive patients, with and without lipohyalinosis. METHOD: Forty patients with essential hypertension were selected at random, 20 with lipohyalinosis in the lenticulostriate arteries (HH group) and 20 without lipohyalinosis (H group), matched with 20 normotensive controls (C group). RESULTS: Plasma protein deposits were identified in eight patients (40 percent) in the C group, in 15 patients (75 percent) in the H group, and in all 20 patients (100 percent) in the HH group, the difference being significant for the H group and highly significant for the HH group, as compared with the C group. In all groups, the distribution of plasma protein deposits, subendothelial in normal arteries, and diffuse, irregular in the wall of arteries with lipohyalinosis, was more frequent in the lenticulostriate arteries of the putamen. CONCLUSION: Deposition of plasma proteins in the lenticulostriate arteries seems to be relatively frequent in normotensive individuals, starting in middle age. Such process appears to be intensified by hypertension, especially in individuals with lipohyalinosis.

PROPÓSITO: Investigar, por meio de método imuno-histoquímico, a deposição de proteínas plasmáticas na parede das artérias lentículo-estriadas, corticais e leptomeníngeas em pacientes com hipertensão arterial, com e sem lipo-hialinose. MÉTODO: Quarenta pacientes com hipertensão arterial foram selecionados aleatoriamente, sendo 20 com lipo-hialinose nas artérias lentículo-estriadas (grupo HH) e 20 sem lipo-hialinose (grupo H), pareados com 20 controles normotensos (grupo C). RESULTADOS: Depósitos de proteínas plasmáticas foram identificados em oito pacientes (40 por cento) do grupo C, em 15 pacientes (75 por cento) do grupo H e em todos os 20 pacientes (100 por cento) do grupo HH, a diferença sendo significativa para o grupo H e altamente significativa para o grupo HH, quando comparada com o grupo C. Em todos os grupos, a distribuição dos depósitos de proteínas plasmáticas, subendotelial em artérias normais e difusa, irregular, na parede das artérias com lipo-hialinose, foi mais freqüente nas artérias lentículo-estriadas do putâmen. CONCLUSÃO: A deposição de proteínas plasmáticas nas artérias lentículo-estriadas parece ser um fenômeno relativamente freqüente em indivíduos normotensos, a partir da meia-idade. Tal processo parece ser intensificado pela hipertensão arterial, particularmente naqueles pacientes com lipo-hialinose.

Proteinograma sérico de veados-catingueiro (Mazama gouazoubira) criados em cativeiro obtido por eletroforese em gel de agarose e de poliacrilamida (SDS PAGE) / Serum protein concentrations of captive brown brocket deer (Mazama gouazoubira) determined by means of agarosis and sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) gel electrophoresis

The serum protein concentrations of brown brocket deer (Mazama gouazoubira) obtained by agarosis gel and sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) gel were determined from blood samples of ten adult healthy animals (six females and four males), monthly collected in the morning, during 12 months. The animals, maintained in individual stable and protected from noise, received ad libitum a diet of comercial ration and green roughage. Serum protein concentrations in agarosis gel revealed the presence of four protein fractions: albumin, alphaglobulin, betaglobulin, and gammaglobulin. Only serum concentrations of albumin were influenced by season, being values in spring higher than values in summer (4.15 x 3.64g/dl). Serum concentrations of albumin (4.05 x 3.75g/dl) were higher for female and alphaglobulin (0.39 x 0.53g/dl) werehigher for males. Results showed 34 proteins with molecular weights ranging from 18kD to 165kD. Significant differences between at least two seasons were found on values of 11 proteins. In conclusion, on account of the 10 animals been maintained in the same physical space and submitted to the same handling system, physiological variations, which are characteristic of this species, can be apointed as the reason of these differences.

Proteinograma sérico de bezerros recém-nascidos da raça Holandesa obtido por eletroforese em gel de poliacrilamida / Serum protein concentration in newborn Holstein calves determined by means of sodium dodecyl sulphate-polyacrylamide gel electrophoresis

The serum protein concentration of newborn Holstein calves determined by means of sodium dodecyl sulphate-polyacrylamide (SDS-PAGE) was studied. Blood samples from 40 healthy newborn calves were obtained 48 hours after birth. Calves had been given 3 liters of colostrum within 2 hours after birth, following by dose corresponding by 15 percent of animal weight each 24 hours. The results showed three different proteinograms: 19 calves had 14 proteins with molecular weights (MW) ranging from 28,000 D to 170,000D (proteinogram 1); 11 calves had 14 proteins with MW ranging from 18,000 to 170,000 D (proteinogram 1); and 10 calves had 12 proteins with MW ranging from 28,000 D to 170,000 D (proteinogram 3). The three groups presented similar IgG levels. The highest serum concentration of ceruloplasmin were verified in proteinogram 3, which had the lowest serum level of protein with MW 58,000D. It was verified a1-antitrypsin only in proteinogram 2, which had no proteins with MW of 42,000 D and 37,000D. The highest serum concentrations of IgA and protein with MW 58,000 D, and the lowest serum levels of transferrin, haptoglobin, and acid glycoprotein were verified in proteinogram 3. Measurement of serum protein concentrations by SDS-PAGE may be useful in monitoring the occurrence of hypogammaglobulinemia and the neonatal disease in calves.

Isolation, identification and characterization of secretory proteins of IVMFC embryos and blood circulation of estrus and early pregnant goat.

The aim of the present study was to isolate, identify and characterize the secretory proteins of IVM oocytes and IVMFC embryos to evaluate its immunogenecity. and identify of such proteins if any, in blood circulation of estrus and early pregnant goats. Oocytes were matured in TCM-199 with 1 microg/ml, estradiol-17beta; 0.5 microg/ml, FSH; 100 IU/ml, LH and 10% FCS on granulosa cell monolayer. After 18 hr of maturation, oocytes were further cultured in maturation medium containing 3 mg/ml polyvinyl alcohol (PVA) without serum and BSA for 12 hr and medium was collected. The IVF embryos of 4-8 cell stage were cultured in medium containing PVA without serum and BSA. Embryo culture medium was collected after 24 hr of culture and was pooled. The proteins were analyzed on SDS-PAGE (12.5%). Four secretory proteins of oocytes with approximately molecular weight of 45, 55, 65 and 95 kDa and three secretory proteins of embryos 45, 55 and 65 kDa were obtained on SDS-PAGE in silver staining. The protein profile of midluteal, estrus and early pregnant goat serum was similar and no variation was observed among the proteins on SDS-PAGE. Two secretory proteins of 55 and 65 kDa of both IVM oocytes and IVMFC embryos were observed on Western analysis. None of such proteins was observed in midluteal, estrus and early pregnant goat serum on western blotting. It can be concluded that IVM oocytes and IVMFC embryos secrete proteins in medium and two of them can develop antibody. The proteins secreted from embryos till morula stage was similar to that of oocytes. None of these oocyte/embryo released proteins were observed in blood circulation of estrus and early pregnant goats.

Aislamiento y caracterizacion de proteinas unidas a prolactina en el plasma de pacientes hiperprolactinemicos / Isolation and characterization of prolactin-binding proteins in plasma of hyperprolactinemic patients

Se analizaron los resultados de la purificación y caracterización de las proteínas que se encuentran unidas a la PRL en el plasma de pacientes hiperprolactinémicos. Se demostró la existencia del complejo proteico mediante incubación del plasma con PRL marcada con yodo radiactivo (PRL-I125), seguido por separación cromatográfica de gel filtración en Sephacryl S-300HR. Se comparó el patrón de elución con proteínas de pesos moleculares conocidos y la radiactividad incorporada se determinó por conteo de las radiaciones gamma. El complejo prolactina-proteína eluyó en un rango de pesos moleculares elevados. Se aislaron las proteínas unidas a la prolactina por cromatografía de afinidad en una columna de prolactina ovina (oPRL) unida a Sepharosa 4B activada con bromuro de cianógeno. La concentración de proteínas en 5 lotes analizados en forma sucesiva, fue de 180"74Fg/fraccionamiento. Las proteínas aisladas mostraron homología inmunológica con la IgG humana mediante el análisis por inmunodifusión radial en geles de agarosa. Según el patrón electroforético encontrado, las proteínas que se unen a la PRL en el plasma están formadas por 4 bandas de pesos moleculares, 2 de las cuales coinciden con las cadenas ligeras y pesadas de la IgG, mientras que las otras 2 presentan pesos moleculares aproximados de 68 y 80 kDa, respectivamente. Los resultados encontrados permiten identificar a las proteínas unida a la PRL como un complejo con una estructura molecular en la cual participa la IgG

La electroforesis de proteínas y su aplicación en la clínica

La experiencia clínica de muchos años ha demostrado que la electroforesis de proteínas en suero es una excelente prueba de laboratorio. Son múltiples las entidades en las que tienen gran importancia clínica. Para su adecuada utilización e interpretación se requiere tener conocimientos acerca de su composición, comportamiento y variaciones de acuerdo a cada entidad nosológica. Las publicaciones sobre este tópico son escasas y las revisiones disponibles fueron publicadas hace muchos años. En este artículo se describen las principales fracciones obtenidas en la electroforesis en acetato de celulosa y se establece una correlación con las principales patologías que la modifican. Además, se definen y esquematizan los patrones electroforéticos mas característicos y de mayor ocurrencia clínica.

Topical fibronectin treatment in persistent corneal epithelial defects and corneal ulcers

Topical fibronectin, autologous and homologous, was used to treat nine patients (eleven eyes) with persistent corneal epithelial defects and corneal ulcers that failed to improve with standard therapy. The fibronectin was purified from autologous and homologous plasma by gelatin-Sepharose 4B affinity chromatography and administered topically, 500 micrograms/ml five times a day, for three weeks. Complete or nearly complete reepithelialization was achieved in all patients regardless of the source of fibronectin, autologous or homologous. But healing times varied. The average healing time was 41.7 +/- 14.7 days (35.7 +/- 12.4 days for autologous, 50.8 +/-14.4 days for homologous). Ocular symptoms were relieved significantly, and no side effects were observed. Over an average follow-up period of 5.2 months, no recurrences were noted. The results showed that homologous, as well as autologous, fibronectin was effective in patients with persistent corneal epithelial defects and corneal ulcers.

Proteinas séricas en niños de diferentes medios socio-económicos / Blood protein in child from different socioeconomic rules

En el estudio realizado sobre proteinas totales, albúminas, globulinas, relación alb./glab. y nitrógeno de los alfa aminoácidos en niños pertenecientes a distintos estractos sociales de la ciudad de Chosica y del Departamento de Lambayeque, encontrándose que los valores medios hallados está dentro de los valores normales dados por el método utilizado. Al efectuar la comparación estadística de los grupos estudiados, se obtuvo valores mayores en los niños de los pueblos jóvenes que en las ciudades de Chosica y Lambayeque, encontrándose una diferencia significativa en el índice de consumo de proteinas.