RESUMO
Arcelin, an anti-metabolic protein was purified from the seeds of wild bean, Lablab purpureus. The feeding assay containing arcelin at 5, 10 and 15 microg concentrations revealed no antifeedant effect against fifth instar larvae of S. litura. However, the enhanced activity of alpha- and beta-naphthyl esterases in the mid-gut samples of S. litura treated with arcelin suggests countermeasure against the toxic effect of arcelin.
Assuntos
Animais , Inseticidas/toxicidade , Proteínas de Plantas/toxicidade , Sementes/química , SpodopteraRESUMO
Histological studies of the spleen and thymus of rats fed raw black beans (Phaseolus vulgaris) show an atrophy of both lymphoid organs. Decrease in relative thymus weight was most marked. All histological organization of this organ appeared altered. An evident decrease in cell number was also observed in both organs. Proliferative response of splenic cells stimulated in vitro with Concanavalin A was increased as compared to that from animals fed the control diet. It is likely that histological changes observed in the spleen and the thymus are due mainly to a protein caloric deficiency, although the possibility that toxic factors present in the raw diet have an effect on the immune system of the rat can not be overruled
Assuntos
Animais , Masculino , Fabaceae , Manipulação de Alimentos , Síndromes de Imunodeficiência/induzido quimicamente , Ativação Linfocitária/efeitos dos fármacos , Baço/efeitos dos fármacos , Timo/efeitos dos fármacos , Atrofia , Peso Corporal/efeitos dos fármacos , Proteínas Alimentares/farmacocinética , Proteínas Alimentares/toxicidade , Inibidores Enzimáticos/toxicidade , Temperatura Alta , Fabaceae/toxicidade , Tamanho do Órgão/efeitos dos fármacos , Fito-Hemaglutininas/toxicidade , Proteínas de Plantas/farmacocinética , Proteínas de Plantas/toxicidade , Ratos , Ratos Sprague-Dawley , Baço/patologia , Taninos/toxicidade , Timo/patologiaRESUMO
The potential participation of PAF-acether (PAF) on the paw oedema triggered by enterolobin was investigated. Intraplantar injections of enterolobin )5-20 µg/paw) yielded a dose response curve for edema which appeared after 30 min, peaked in the interval between 2-4 h and faded after 24h. The pre-treatment with BN 52021, but not with other PAF antagonists such as PCA 4248 or WEB 2086, significantly blocked enterolobin-induced oedema. To clarify better the discrepant results obtained with the PAF antagonists, desensitization to PAF was performed. The oedema triggered by enterolobin was not modified in paf desensitized animals. It was concluded that the paw inflammation induced by enterolobin does not require PAF mechanism.