RESUMO
Carotenoids are efficient antioxidants that are of great importance for human health. Lutein and zeaxanthin are carotinoids present in high concentrations in the human retina which are involved in the photoprotection of the human eye. Lutein may also protect the skin from ultraviolet (UV)-induced damage. The present study investigated the protective effect of lutein extracted from yellow silk cocoons of Bombyx mori on human keratinocytes against UVB irradiation. A human keratinocyte cell line and primary human keratinocytes were used to investigate the UVB protection effects of silk lutein and plant lutein. Silk lutein showed no cytotoxicity to keratinocytes. Treatment with silk lutein prior to UVB irradiation enhanced cell viability and cell proliferation, and reduced cell apoptosis. The protective effects of silk lutein may be superior to those of plant lutein. Silk lutein may have a benefit for protection of keratinocytes against UVB-irradiation.
Assuntos
Animais , Humanos , Masculino , Queratinócitos/efeitos da radiação , Luteína/farmacologia , Protetores contra Radiação/farmacologia , Seda/química , Raios Ultravioleta/efeitos adversos , Apoptose/efeitos dos fármacos , Apoptose/efeitos da radiação , Bombyx/metabolismo , Linhagem Celular , Proliferação de Células/efeitos dos fármacos , Proliferação de Células/efeitos da radiação , Sobrevivência Celular/efeitos da radiação , Prepúcio do Pênis/efeitos da radiação , Luteína/isolamento & purificação , Cultura Primária de Células , Protetores contra Radiação/isolamento & purificaçãoRESUMO
Echinacea (E.) purpurea herb is commonly known as the purple coneflower, red sunflower and rudbeckia. In this paper, we report the curative efficacy of an Echinacea extract in gamma-irradiated mice. E. purpurea was given to male mice that were divided into five groups (control, treated, irradiated, treated before irradiation & treated after irradiation) at a dose of 30 mg/kg body weight for 2 weeks before and after irradiation with 3 Gy of gamma-rays. The results reflected the detrimental reduction effects of gamma-rays on peripheral blood hemoglobin and the levels of red blood cells, differential white blood cells, and bone marrow cells. The thiobarbituric acid-reactive substances (TBARs) level, Superoxide dismutase (SOD) and glutathione peroxidase (GSPx) activities and DNA fragmentation were also investigated. FT-Raman spectroscopy was used to explore the structural changes in liver tissues. Significant changes were observed in the microenvironment of the major constituents, including tyrosine and protein secondary structures. E. purpurea administration significantly ameliorated all estimated parameters. The radio-protection effectiveness was similar to the radio-recovery curativeness in comparison to the control group in most of the tested parameters. The radio-protection efficiency was greater than the radio-recovery in hemoglobin level during the first two weeks, in lymphoid cell count and TBARs level at the fourth week and in SOD activity during the first two weeks, as compared to the levels of these parameters in the control group.
Assuntos
Animais , Masculino , Camundongos , Antioxidantes/isolamento & purificação , Contagem de Células Sanguíneas , Fragmentação do DNA/efeitos dos fármacos , Echinacea/química , Eritrócitos/efeitos dos fármacos , Raios gama , Glutationa Peroxidase/metabolismo , Leucócitos/efeitos dos fármacos , Peroxidação de Lipídeos/efeitos dos fármacos , Fígado/efeitos dos fármacos , Fitoterapia , Extratos Vegetais/farmacologia , Protetores contra Radiação/isolamento & purificação , Distribuição Aleatória , Superóxido Dismutase/metabolismoRESUMO
We examined the radioprotective effect of aminothiol 2-N-propylamine-cyclo-hexanethiol (20-PRA) on a human leukemic cell line (K562) following various radiation doses (5, 7.5 and 20 Gy) using a source of (60)Co gamma-rays. At 5 Gy and 1 nM 20-PRA, a substantial protective effect (58 per cent) was seen 24 h after irradiation, followed by a decrease at 48 h (11 per cent). At the high radiation dose (20 Gy) a low protective effect was also seen (35 per cent). In addition, the antitumorigenic potential of 10 nM 20-PRA was shown by the inhibition of crown gall formation induced by Agrobacterium tumefaciens. The radioprotective potency of 20-PRA is 10(5)-10(6) times higher than that of the aminothiol WR-1065 N-(2-mercaptoethyl) - 1,3-diaminopropane) whose protective effect is in the 0.1 to 1.0 mM range.
Assuntos
Humanos , Animais , Mercaptoetilaminas/farmacologia , Protetores contra Radiação/farmacologia , Protetores contra Radiação/isolamento & purificação , Células Tumorais Cultivadas/efeitos dos fármacosRESUMO
A series of studies has been carried out in the field of traditional medicine for searching radio-protective agents. According to the theory of traditional Chinese medicine, may prescriptions were tested with experimental animals. Some of them could raise the survival rate of dogs irradiated with lethal dose of Pi-rays by 30-40%. Some symptoms of radiation sickness could be improved. More than one thousand kinds of Chinese herbs were screened. Some of them have pronounced radioprotectice activities. A series of bioactive components wee isolated from these herbs. The mechanism of radiation protection were studied. Having the capability of hemopoietic system and immune system may be the characteristics of these Chinese herbs.