RESUMO
Suid herpesvirus 1 (SuHV-1) is the causative agent of pseudorabies (PR), a disease of great importance due to the huge losses it causes in the swine industry. The aim of this study was to determine a method for genotyping SuHV-1 based on partial sequences of the gene coding for glycoprotein C (gC) and to elucidate the possible reasons for the variability of this region. A total of 109 gCsequences collected from GenBank were divided into five major groups after reconstruction of a phylogenetic tree by Bayesian inference. The analysis showed that a portion of gC (approximately 671 bp) is under selective pressure at various points that coincide with regions of protein disorder. It was also possible to divide SuHV-1 into five genotypes that evolved under different selective pressures. These genotypes are not specific to countries or continents, perhaps due to multiple introduction events related to the importation of swine.
Assuntos
Animais , Variação Genética , Glicoproteínas/genética , Herpesvirus Suídeo 1/genética , Herpesvirus Suídeo 1/patogenicidade , Pseudorraiva/genética , Sequência de Bases/genética , Varicellovirus/genética , Varicellovirus/patogenicidade , Genética Microbiana , Genótipo , Métodos , VirulênciaRESUMO
Seis amostras do vírus da doença de Aujeszky (VDA), isoladas nas regiöes Sul e Sudeste do Brasil foram analisadas e comparadas entre si entre as amostras avirulentas Bartha e NIA-4. Quando se consideraram o número e, principalmente, o tamanho e o padräo de migraçäo dos fragmentos obtidos com as endonucleases de restriçäo Bam HI, Sall, Pvull, KpnI e PstI, as amostras brasileiras puderam ser arranjadas em grupos genômicos e diferenciadas das amostras vacinais. Os achados mostraram que amostras de uma mesma regiäo apresentam perfis genômicos semelhantes. A fragmentaçäo genômica poderá ser de grande importância em análises epidemiológicas do VDA no Brasil