Assuntos
Humanos , Animais , Camundongos , Biotecnologia , Quimeras de Transplante/imunologia , Transplante Heterólogo/efeitos adversos , Transplante Heterólogo/fisiologia , Transplante Heterólogo/imunologia , Transplante Heterólogo/métodos , Transplante Heterólogo/psicologia , Transplante Heterólogo , Rejeição de Enxerto , Haplorrinos , Mutação/genética , SuínosRESUMO
The aim of this study was to evaluate microchimerism after human liver transplantation (LT). This study included 13 female recipients who received hepatic allograft from male donors at Asan Medical Center. A nested PCR specific for Y-chromosome gene (DYZ3) was used to analyze the small number of male cells in the peripheral blood mononuclear cells of the female recipients. Microchimerism was observed in 6 of 13 recipients and 16 out of 35 samples. Only 3 patients showed microchimerism 3 months after LT. There was no statistical difference between the presence of microchimerism and clinical findings such as type of donor, type of immunosuppression, episode of rejection and age of recipient. This study did not show any clinical relevance of microchimerism and further larger study are needed to confirm the results.
Assuntos
Adulto , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Adolescente , Animais , Transplante de Fígado , Linfócitos/imunologia , Quimeras de Transplante/imunologia , Quimeras de Transplante/genética , Cromossomo YRESUMO
The aim of this study was to evaluate microchimerism after human liver transplantation (LT). This study included 13 female recipients who received hepatic allograft from male donors at Asan Medical Center. A nested PCR specific for Y-chromosome gene (DYZ3) was used to analyze the small number of male cells in the peripheral blood mononuclear cells of the female recipients. Microchimerism was observed in 6 of 13 recipients and 16 out of 35 samples. Only 3 patients showed microchimerism 3 months after LT. There was no statistical difference between the presence of microchimerism and clinical findings such as type of donor, type of immunosuppression, episode of rejection and age of recipient. This study did not show any clinical relevance of microchimerism and further larger study are needed to confirm the results.