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Journal of Southern Medical University ; (12): 678-681, 2011.
Artigo em Chinês | WPRIM | ID: wpr-332576

RESUMO

<p><b>OBJECTIVE</b>To investigate the effects of Trichophyton rubrum exposure on the expressions of toll-like receptor-2 (TLR-2), TLR-4 and dendritic cell associated C-type lectin-1 (Dectin-1) and cytokine secretions in human keratinocytes cell line HaCaT.</p><p><b>METHODS</b>The mRNA of TLR-2,4, and dectin-1 in the HaCaT co-cultured with the conidia of Trichophyton rubrum conidia for 24 h was measured with real-time PCR. The mean fluorescence intensity and the percentage of cells positive for TLR-2, 4, and dectin-1 was detected during the co-culture using flow cytometry. The cytokine secretion profiles in the cell culture supernatant was analyzed using a cytokine antibody array.</p><p><b>RESULTS</b>The TLR-2,4, and dectin-1 mRNA expressions, mean fluorescence intensity and percentage of positive cells for TLR-2,4, and dectin-1 all increased in HaCaT cells in response to Trichophyton rubrum conidia exposure. The results of cytokine antibody array demonstrated obviously increased secretions of IL-8, I-309, IFN-γ, IL-6, and IL-13 in the culture supernatant of HaCaT cells in response to Trichophyton rubrum exposure.</p><p><b>CONCLUSION</b>The immune responses and immunological recognition of human keratinocytes to Trichophyton rubrum conidia are partially mediated by up-regulating the expressions of TLR-2, TLR-4 and dectin-1 and secretions of multiple cytokines.</p>


Assuntos
Humanos , Linhagem Celular , Quimiocina CCL1 , Secreções Corporais , Técnicas de Cocultura , Interferon gama , Secreções Corporais , Interleucina-13 , Secreções Corporais , Interleucina-6 , Secreções Corporais , Interleucina-8 , Secreções Corporais , Queratinócitos , Metabolismo , Lectinas Tipo C , Metabolismo , Receptor 2 Toll-Like , Metabolismo , Receptor 4 Toll-Like , Metabolismo , Trichophyton , Fator de Necrose Tumoral alfa , Secreções Corporais
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