Influência do equipamento ondas curtas na produção de estresse oxidativo e capacidade antioxidante / Influence of short waves equipment in the production of oxidative stress and antioxidant capacity

Objetivo - A maioria dos estudos sobre produção de estresse oxidativo tem se voltado para a prática de exercícios aeróbios e relações com diversas patologias. Uma análise detalhada de indivíduos submetidos à radiação eletromagnética produzida pelo equipamento de ondas curtas proporcionará grande contribuição para a compreensão da relação estresse oxidativo e radiação eletromagnética. O objetivo deste estudo é avaliar a influência do campo eletromagnético emitido pelo equipamento de ondas curtas na produção de estresse oxidativo e a capacidade antioxidante de cada indivíduo. Métodos - Foram selecionados 15 indivíduos universitários, independente do gênero, de 18 a 25 anos de idade, submetidos a sessões diárias em aparelho de ondas curtas contínuas durante 15 minutos. Foi aplicado questionário, e antes e após as 10 sessões coletada uma amostra de sangue venoso, para análise e comparação dos níveis de Malondialdeído (MDA) e CapacidadeAntioxidante Total (TEAC). Resultados - Nos indivíduos em geral ocorreu aumento da média de MDA, porém nos indivíduos etilistas ocorreu um aumento significante com P=0,0432. Naqueles que incluíam em sua dieta verduras e legumes, frutas e margarina, ocorreu aumento de TEAC significante com P=0,0328, P=0,0360, P=0,0275, respectivamente. Em indivíduos que apresentaram IMC entre 24,8 - 28,0 ocorreu aumento significante de TEAC com P=0,0316. Conclusões - Conclui-se que o ondas curtas não interfere na produção de estresse oxidativo devido à ação imediata da capacidade antioxidante.

Objective - Most studies on production of oxidative stress has been returned to the practice of aerobic exercises and relations with various pathologies. A detailed analysis of individuals subjected to electromagnetic radiation produced by short wave equipment will provide great contribution to the understanding of oxidative stress and electromagnetic radiation. The purpose of this study is to assess the influence of electromagnetic emitted by short-wave equipment in the production of oxidative stress and antioxidant capacity of each individual. Methods - For this, 15 university individuals were selected, regardless of gender, from 18 to 25 years old, underwent daily sessions in short-wave equipment continued for 15 minutes. Questionnaire was applied, and before and after the 10 sessions collected a sample of venous blood for analysis and comparison of levels of Malondialdehyde (MDA) and Total Equivalente Antioxidant Capacity (TEAC). Results - In subjects in general there was an increase in average MDA, but in subjects alcohol was a significant increase with P = 0.0432. Those in their diet that included vegetables and legumes, fruits, nuts and margarine, there was significant increase in TEAC with P = 0.0328, P = 0.0360, P = 0.0275,respectively. In individuals who had BMI between 24.8 - 28.0 occurred with a significant increase of TEAC P = 0.0316. Conclusions - It was concluded that the short waves do not interfere with the production of oxidative stress due to the immediate action of antioxidant capacity.

An extract of Polygonum multiflorum protects against free radical damage induced by ultraviolet B irradiation of the skin

Over the last decades, the incidence of ultraviolet B (UVB)-related skin problems has been increasing. Damages induced by UVB radiation are related to mutations that occur as a result of direct DNA damage and/or the production of reactive oxygen species. We investigated the anti-oxidant effects of a Polygonum multiflorum thumb extract against skin damage induced by UVB irradiation. Female SKH-1 hairless mice were divided into three groups: control (N = 7), distilled water- (N = 10), and P. multiflorum extract-treated (PM, N = 10) groups. The PM (10 g) was extracted with 100 mL distilled water, cryo-dried and 9.8 g was obtained. The animals received a topical application of 500 æL distilled water or PM extract (1, 2, 4, 8, and 16 percent, w/v, dissolved in distilled water) for 30 min after UVB irradiation (wavelength 280-320 nm, 300 mJ/cm²; 3 min) of the dorsal kin for 14 days, and skin immunohistochemistry and Cu,Zn-superoxide dismutase (SOD1) activity were determined. SOD1 immunoreactivity, its protein levels and activities in the skin were significantly reduced by 70 percent in the distilled water-treated group after UVB irradiation compared to control. However, in the PM extract-treated groups, SOD1 immunoreactivity and its protein and activity levels increased in a dose-dependent manner (1-16 percent, w/v, PM extract) compared to the distilled water-treated group. SOD1 protein levels and activities in the groups treated with 8 and 16 percent, w/v, PM extract recovered to 80-90 percent of the control group levels after UVB. These results suggest that PM extract strongly inhibits the destruction of SOD1 by UV radiation and probably contains anti-skin photoaging agents.

Radicales libres, alcoholismo y daño hepatico / Free radicals, alcoholism and hepatic damage

En el presente artículo se presenta una revisión acerca del papel de los radicales libres en la producción de dano celular y tisular en general, pero particulaemente en la producción de dano a nivel hepático inducido por el alcohol. Al mismo tiempo se revisa lo que son los radicales libres, las principales reacciones químicas que llevan a su formación en el organismo, así como, los distintos mecanismos de defensa que tiene este último para enfrentarlos