Deacidification of lampante olive oil

Deacidification of lampante olive oil by anion exchange resin and aluminum oxide was investigated. The experimental results proved that it is feasible to obtain a refined olive oil with free fatty acids less than 0.3% using anion exchange column. Efficiency of anion exchange resin as a deacidification material was 4 times as that of the aluminum oxide. Peroxides of the investigated olive oil were decomposed by heat treatment of the deacidified oil in presence of a stream of N[2]. K[232], K[270] and delta-K of the refined olive oil were 2.37,0.89 and 0.02, respectively. The results revealed that the untreated olive oil and the oil constituents that retained on the resin exhibited an efficient cytotoxicity against breast carcinoma cell line [MCF7] with IC[50] values of 61.1 and 70.5 micro g, respectively, while deacidified oil did not maintain this property

Optimization of purification conditions for with macroporous adsorption resin total saponins from smilax china / 中国中药杂志

<p><b>OBJECTIVE</b>To develop a with high efficiency and practicality for separating and purifying total steroidal saponins lax china.</p><p><b>METHOD</b>Using adsorption capacity and desorption rate of total steroidal saponins as the primary screening index, surveyed, and the optimized conditions of adsorption and desorption of total steroidal saponins were studied.</p><p><b>RESULT</b>The adsorption and desorption rate of total steroidal saponins reached 16 mg x mL(-1) and 90% respectively for macroporous resin HPD100 chosen. Macroporous resin HPD100 could be well used in separating and purifying total steroidal saponins from S. china.</p>

Anion exchange resin as support for invertase immobilization

A invertase (EC 3.2.1.26) de Saccharomyces cerevisiae foi usada como enzima modelo na avaliação da capacidade de adsorção da DOWEX-1X8-50(marca registrada),uma resina trocadora de ânions, para emprego como suporte na técnica de imobilização.Obteve-se 93 por cento de adsorção ao misturar-se 100mg da resina com 27mg de invertase (pI = 4,0)sob condições definidas de imobilização (pH 4,6, agitação de 100rpm e 25oC).As atividades (1U = mg de açúcares redutores formados/min) determinadas para a invertase solúvel e imobilizada foram iguais a 0,084 U/ mgE e 0,075 U/mgE, respectivamente.O coeficiente de imobilização obtido foi de 90.4 por cento.A termoestabilidade da invertase imobilizada foi maior do que a da enzima solúvel.O pH de maior atividade para ambas as formas da enzima foi 4,5, e as faixas de pH de maior estabilidade foram 3,5-5,0 e 4,5-5,5, respectivamente, para a invertasesolúvel e imobilizada.As constantes cinéticas para a invertase solúvel foram KM = 18,3 mM e Vmax = 0,084 U/ mgE, enquanto que para a forma imobilizada foram KM = 29,1 mM e Vmax = 0,075 U/mgE.A resina testada mostrou ter alta capacidade adsorvente, quando a molécula da invertase está carregada negativamente, ou seja, a imobilização e a reação de hidrólise conduzidas em pH > pI

Study on adsorption of danshen water-soluble compounds with macroporous resin D301 / 中国中药杂志

<p><b>OBJECTIVE</b>To study the adsorption and enrichment of effective compounds of danshensu, and protocatechualdehyde from Danshen water extracts by macroporous resin D301.</p><p><b>METHOD</b>The change of active compounds was observed by detecting the amount of danshensu and protocatechualdehyde with HPLC.</p><p><b>RESULT AND CONCLUSION</b>Macroporous resins D301 can be used in the process of adsorbing and purifying active compounds of danshensu and protocatechualdehyde, and increase the amount of active compounds in water extracts.</p>

4 Cases of Pseudomembranous Colitis Confirmed by Clostridium Culture / 대한소화기내시경학회지

Diarrhea is a common complication of antibiotics usage. The diarrhea ascribed to antibiotics usually consist of loose or watery stools, sometimes containing mucus but rarely grossly evident blood. Almost every antibiotics has been implicated, although cases related to the use of vancomycin or aminoglycosides are rare. Although the pathogenesis of antibiotics-associated diarrhea is not confirmed, recent studies have shown that a toxin of clostridium difficile is implicated as a cause of it. Diagnosis is made by detecting toxin in the stool or stool culture for C.difficile. Treatment is either directed at binding the toxin with anion exchange resins such as cholestyramine in mild case, or at eradicating the C.difficile organism with vancomycin, metronidazole or bacitracin for more severe cases. (continue...)