Xylitol concentrations in artificial saliva after application of different xylitol dental varnishes

OBJECTIVE: The present study analyzed xylitol concentrations in artificial saliva over time after application of varnishes containing 10% and 20% xylitol. MATERIAL AND METHODS: Fifteen bovine enamel specimens (8x4 mm) were randomly allocated to 3 groups (n=5/group), according to the type of varnish used: 10% xylitol, 20% xylitol and no xylitol (control). After varnish application (4 mg), specimens were immersed in vials containing 500 µL of artificial saliva. Saliva samples were collected in different times (1, 8, 12, 16, 24, 48 and 72 h) and xylitol concentrations were analyzed. Data were assessed by two-way repeated-measures ANOVA (p<0.05). RESULTS: Colorimetric analysis was not able to detect xylitol in saliva samples of the control group. Salivary xylitol concentrations were significantly higher up to 8 h after application of the 20% xylitol varnish. Thereafter, the 10% xylitol varnish released larger amounts of that polyol in artificial saliva. CONCLUSIONS: Despite the results in short-term, sustained xylitol releases could be obtained when the 10% xylitol varnish was used. These varnishes seem to be viable alternatives to increase salivary xylitol levels, and therefore, should be clinically tested to confirm their effectiveness.

Leite bovino pasteurizado, saliva artificial ou clara do ovo de galinha como meios para manter dentes avulsionados: estudo histológico em ratos / Pasteurized cow milk, artificial saliva, or chicken egg as storage media of avulsed teeth: histological study in rats

Reimplantaram-se 54 incisivos de ratos, que foram mantidos imersos por 2 horas no leite bovino pasteurizado, na saliva artificial ou na clara do ovo de galinha. Transcorridos 10, 30 e 60 dias após o reimplante, os animais foram sacrificados. As peças obtidas foram tratadas laboratorialmente e os cortes, corados com hematoxilina e eosina, para realizar a análise histológica. Os resultados foram tratados estatisticamente. As reabsorçöes radiculares sem substituiçäo foram significantes aos 60 dias nos dentes mantidos na clara do ovo e na saliva, quando comparado com o leite bovino pasteurizado. As reabsorçöes radiculares com substituiçäo por tecido duro (cemento e/ou osso) foram significantes aos 30 e 60 dias nos dentes mantidos na clara do ovo, quando comparados com os mantidos no leite ou na saliva. Os resultados histológicos demonstraram que o leite bovino é superior à clara do ovo e à saliva artificial para manter a vitalidade dos remanescentes do ligamento periodontal cementário (LPC). A saliva artificial näo manteve a vitalidade dos remanescentes do LPC, tendo sido substituídos por tecido conjuntivo. Nenhum dos meios manteve a vitalidade da polpa