Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Adicionar filtros








Intervalo de ano
1.
Biol. Res ; 38(1): 69-82, 2005. ilus, graf
Artigo em Inglês | LILACS | ID: lil-404829

RESUMO

We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80 percent homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.


Assuntos
Animais , /biossíntese , DNA Bacteriano/genética , /biossíntese , Piscirickettsiaceae/imunologia , Salmão/microbiologia , Anticorpos Antibacterianos/imunologia , Sequência de Bases , /genética , Biblioteca Genômica , /genética , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Salmão/imunologia
2.
Biol. Res ; 37(4,supl.A): 783-793, 2004. ilus
Artigo em Inglês | LILACS | ID: lil-399658

RESUMO

We have isolated and sequenced the genes encoding the membrane bound transglycosylase B (MltB) and the transferring binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis. The results of the sequence revealed two open reading frames that encode proteins with calculated molecular weights of 38,830 and 85,140. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from phylogenetically related microorganisms. Partial sequences coding the amino and carboxyl regions of MltB and a sequence of 761 base pairs encoding the amino region of TbpB have been expressed in E. coli. The strong humoral response elicited by these proteins in mouse confirmed the immunogenic properties of the recombinant proteins. A similar response was elicited by both proteins when injected intraperitoneally in Atlantic salmon. The present data indicates that these proteins are good candidates to be used in formulations to study the protective immunity of salmon to infection by P. salmonis.


Assuntos
Masculino , Animais , Camundongos , Código Genético/genética , Glicosiltransferases/genética , Piscirickettsiaceae/enzimologia , Salmão/microbiologia , Proteína B de Ligação a Transferrina , Sequência de Bases , Técnicas de Cultura de Células , Ensaio de Imunoadsorção Enzimática , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Piscirickettsiaceae/genética , Piscirickettsiaceae/imunologia , Proteínas de Membrana/genética , Salmão/imunologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA