RESUMO
OBJECTIVE@#Osteosarcoma(OS) and Ewing's sarcoma (EWS) are the two most common primary malignant bone tumors in children. The aim of the study was to identify key genes in OS and EWS and investigate their potential pathways.@*METHODS@#Expression profiling (GSE16088 and GSE45544) were obtained from GEO DataSets. Differentially expressed genes were identified using GEO2R and key genes involved in the occurrence of both OS and EWS were selected using venn diagram. Gene ontology and pathway enrichment analyses were performed for the ensembl. Protein-protein interaction (PPI) networks were established by STRING. Further, UCSC was used to predict the transcription factors of the cell division cycke 5-like(CDC5L) gene, and GEPIA was used to analyze the correlation between the transcription factors and the CDC5L gene.@*RESULTS@#The results showed that CDC5L gene was the key gene involved in the pathogenesis of OS and EWS. The gene is mainly involved in mitosis, and is related to RNA metabolism, processing of capped intron-containing pre-mRNA, mRNA and pre-mRNA splicing.@*CONCLUSION@#CDC5L, as a key gene, plays a role in development of OS and EWS, which may be reliable targets for diagnosis and treatment of these primary malignant tumors.
Assuntos
Criança , Humanos , Neoplasias Ósseas/patologia , Proteínas de Ciclo Celular/genética , Biologia Computacional , Perfilação da Expressão Gênica , Osteossarcoma/genética , Proteínas de Ligação a RNA/genética , Sarcoma de Ewing/genéticaRESUMO
Extraskeletal Ewing's sarcoma (EES) is a rare soft tissue tumor morphologically indistinguishable from the more common Ewing's sarcoma of bone. We report a case of EES arising in the hard palate of 34-yr-old male patient. Microscopically, the monotonous small round cells without neuronal differentiation showed membranous positive immunoreactivity for MIC2/CD99 and vimentin. Ultrastructurally, the tumor cells showed a few intracytoplasmic organelles without evidence of neurosecretory granules or neurofilaments. The EWS-FLI1 chimeric gene was identified using the nested reverse transcriptase-polymerase chain reaction.
Assuntos
Adulto , Humanos , Masculino , Antígenos CD/análise , Moléculas de Adesão Celular/análise , Imuno-Histoquímica , Proteínas de Fusão Oncogênica/genética , Neoplasias Palatinas/genética , Palato Duro/metabolismo , Proteína Proto-Oncogênica c-fli-1/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sarcoma de Ewing/genética , Transcrição Gênica , Vimentina/análiseRESUMO
Alguns aspectos da citogenética do câncer säo discutidos aqui. Rearranjos cromossômicos säo agrupados em três categorias, baseadas no mecanismo pelo qual elas promovem o desenvolvimento do tumor: a) translocaçöes, inversöes e inserçöes; b) deleçöes e monossomias cromossômicas; c) amplificaçöes. "Imprinting" genômico, tumores benignos e metástases säo também discutidos