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1.
Indian J Exp Biol ; 2006 Apr; 44(4): 340-4
Artigo em Inglês | IMSEAR | ID: sea-57987

RESUMO

Nine halophilic archaea viz., Halobacterium salinarum, Halobacterium sp.1, Halobacterium sp.2, Halobaculum sp., Halococcus saccharolyticus, Halorubrum saccharovorum, Haloterrigena turkmenica, Halogeometricum sp. and Natrialba sp. isolated from marine salterns around Bhavnagar coast were screened for siderophore production. Five isolates viz., Halococcus saccharolyticus, Halorubrum saccharovorum, Haloterrigena turkmenica, Halogeometricum sp. and Natrialba sp. produced siderophores as evidenced by positive reaction in FeCl3 test, CAS assay and CAS agar plate test. Determination of chemical nature of siderophores by chemical assays and bioassays identified them as carboxylates. Quantification of siderophores indicated Halorubrum saccharovorum to be the maximum siderophore producer (2.62 RE mg/ml) and Halococcus saccharolyticus to be the least (1.33 RE mg/ml). The present study is the first report on siderophore production in Indian haloarchaeal strains. Mechanism of iron assimilation in four non-siderophore isolates still needs to be investigated further.


Assuntos
Halobacterium/química , Sideróforos/química
2.
Indian J Exp Biol ; 2004 Jan; 42(1): 96-105
Artigo em Inglês | IMSEAR | ID: sea-58769

RESUMO

Thirtyfive siderophore producing fungi were categorized for their hydroxamate, catecholate or carboxylate nature by chemical and bioassays. Out of 35 fungi, 30 were hydroxamates and 5 showed carboxylate nature. However, none of the fungi produced catecholate type of siderophores. Eighteen out of 29 fungi were trihydroxamate and the rest 11 fungi were dihydroxamates. Twenty-three fungi were hexadentate and 6 were tetradentate in nature. Quantification of siderophores using standard compounds deferrioxamine mesylate and rhizoferrin revealed that Phanerochaete chrysosporium produced maximum among the hydroxamate producing fungi and Mycotypha africana resulted maximum among the carboxylate producing fungi.


Assuntos
Bioensaio , Ácidos Carboxílicos/química , Desferroxamina/química , Relação Dose-Resposta a Droga , Epinefrina/análogos & derivados , Compostos Férricos/química , Fungos/metabolismo , Ácidos Hidroxâmicos/química , Ferro/metabolismo , Ligantes , Sideróforos/química , Espectrofotometria
3.
Indian J Exp Biol ; 2000 Jan; 38(1): 56-62
Artigo em Inglês | IMSEAR | ID: sea-57950

RESUMO

Siderophores of twenty fungi belonging to Zygomycotina (5 Mucorales), Ascomycotina (7 aspergilli, 6 penicillia, Neurospora crassa) and Deuteromycotina (Fusarium dimerum) were examined for their chemical nature. Siderophores produced by fungi other than Mucorales were all hydroxamates. Mucorales produced carboxylate siderophores. Catecholate type of siderophores were not detectable. Hydroxamate siderophores were mostly (9 out of 15) trihydroxamates, while six were dihydroxamates. Monohydroxamate nature was not shown by any of the 15 test fungal siderophores. In ligand properties, 12 out of 15 hydroxamate siderophores formed hexadentate ligands, while two formed tetradentates and one bidentate. There was good correlation between number of hydroxamate groups and ligand property.


Assuntos
Aspergillus/química , Ácidos Carboxílicos/química , Fungos/química , Fusarium/química , Ácidos Hidroxâmicos/química , Mucorales/química , Neurospora crassa/química , Penicillium/química , Sideróforos/química , Especificidade da Espécie
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