RESUMO
The study evaluated cefoxitin disk diffusion tests breakpoints and their correlation to mecA gene PCR results for detecting Methicillin-resistant Staphylococcus intermedius Group (MRSP) isolates from dogs in Brazil. Agreement using proposed breakpoint (resistant < 30 mm) was encouraging. The current study reinforces that an epidemiological breakpoint can be established to predict presence of MRSP.
Assuntos
Animais , Cães , Cefoxitina , Farmacorresistência Bacteriana , Resistência a Meticilina , Staphylococcus intermedius , Reação em Cadeia da Polimerase , Testes de Sensibilidade a Antimicrobianos por Disco-Difusão/veterináriaRESUMO
The Staphylococcus (S.) intermedius group (SIG) has been a main research subject in recent years. S. pseudintermedius causes pyoderma and otitis in companion animals as well as foodborne diseases. To prevent SIG-associated infection and disease outbreaks, identification of both staphylococcal exotoxins and staphylococcal cassette chromosome mec (SCCmec) types among SIG isolates may be helpful. In this study, it was found that a single isolate (one out of 178 SIG isolates examined) harbored the canine enterotoxin SEC gene. However, the S. intermedius exfoliative toxin gene was found in 166 SIG isolates although the S. aureus-derived exfoliative toxin genes, such as eta, etb and etd, were not detected. SCCmec typing resulted in classifying one isolate as SCCmec type IV, 41 isolates as type V (including three S. intermedius isolates), and 10 isolates as non-classifiable. Genetic relatedness of all S. pseudintermedius isolates recovered from veterinary staff, companion animals, and hospital environments was determined by pulsed-field gel electrophoresis. Strains having the same band patterns were detected in S. pseudintermedius isolates collected at 13 and 18 months, suggesting possible colonization and/or expansion of a specific S. pseudintermedius strain in a veterinary hospital.
Assuntos
Animais , Gatos , Cães , Humanos , Toxinas Bacterianas/genética , Doenças do Gato/epidemiologia , Cromossomos Bacterianos/genética , Doenças do Cão/epidemiologia , Eletroforese em Gel de Campo Pulsado/veterinária , Enterotoxinas/genética , Exfoliatinas/genética , Exotoxinas/genética , Hospitais Veterinários , Corpo Clínico Hospitalar , Dados de Sequência Molecular , Animais de Estimação/microbiologia , Reação em Cadeia da Polimerase/veterinária , República da Coreia/epidemiologia , Infecções Estafilocócicas/epidemiologia , Staphylococcus/genética , Staphylococcus intermedius/genéticaRESUMO
Bacteriophages were induced from staphylococcus intermedius isolates from dogs and used for a phage typing. Mitomycin C induction was performed on 60 strains of S. intermedius and all phages were reacted with the strains. Twenty-eight strains (46.7%) were found to be lysogenic. Based on host ranges, eight phages were selected. By using the eight phages, 129 strains isolated from canine clinical specimens were subjected to the phage typing at the routine test dilution (RTD) and 100xRTD. Typability of the phage set was 78.3%, yielding 40 phage patterns. The phage set was considered useful for differentiating S. intermedius strains isolated from dogs. None of 50 strains of S. aureus, 2 (3.6%) of 56 S. simulance strains, 13 (24.5%) of 53 S. chromogenes strains and 46 (28.1%) of 164 S. hyicus strains were typable by the phages at 100xRTD.