Effect of the black seed oil [Nigella Sativa oil] on experimentally-induced gastric mucosal injury in albino rats

The present study was done to investigate the possible effect of Nigella sativa oil [NSO] on cold restraint [CR] and indomethacin [I] induced gastric ulcer in albino rats. Thirty-six adult male albino rats were used in this study; they were divided into six equal groups. Pyloric ligation was done in all rats to collect gastric juice. Gastric ulcer was induced by CR and I treatment. Oral treatment with NSO daily for two weeks was given to control [non-stressed] and to CR and I treated rats before ulcer induction. Gastric ulcer scores, gastric juice analysis for acidity, proteolytic activity and mucin, gastric mucosa1 histamine, glutathione [GSH], prostaglandin E2 [PGE2] and lipid peroxides as well as serum total antioxidant status [TAS] were determined and the mean levels were compared with control non treated parameters. It can be concluded that NSO imparted a protective action against CR- and I-induced ulcer in rats and can be recommended for prophylaxis and treatment of gastric mucosa1 injury

relationship between nitric oxide [No] and gastric secretion

The role of NO in the regulation of gastric acid secretion was examined in the anaesthetized adult male albino rats. Continuous i.v. infusion of the NO donor, sodium nitroprusside [SNP, 12mg/kg/h] significantly inhibited both basal and stimulated gastric acid secretion which was mediated neuronally by gastric distension [20 cm H2O or i.v. bolus administration of 2-deosxy -D- glucose [150 mg/kg], as well as gastric acid responses induced by i.v infusion of submaximal doses of pentagastrin [8 micro g/kg/h]. By contrast, gastric acid responses to i.v. infusion of submaximal doses of histamine [1 mg/kg/h] were not influenced by the NO donor. Pretreatment with N[G] - nitro-L-arginine methylester[L-NAME, 10mg/kg, i.v.] did not affect basal acid secretion, but significantly potentiated the increase in acid secretion induced by 2-deoxy-D- glucose which was almost completely antagonized by coadministration of L-arginine [500 mg/kg, i.p.] and slightly augmented the acid secretory response to pentagastrin. Moreover, it was found that pentagastrin infusion caused an increase in luminal release of histamine and this response was significantly suppressed by i.v infusion of the NO donor.Intraperitoneal [i.p] administration of cimetidine [60 mg/kg], a histamine H2-receptor antagonist, significantly inhibited the increase in acid secretion in response to 2-deoxy-D-glucose [150 mg/kg, i.v.], pentagastrin [8 micro g/kg/h, i.v] and histamine [I mg/kg/h, i.v.] infusion. In conclusion, the present study suggests that NO has an inhibitory action on gastric acid secretion that may be through suppression of histamine release from enterochromaffin like [ECL] cells

Spot diagnosis of aluminium phosphide ingestion: an application of a simple test.

Silver nitrate impregnated paper test was performed with the gastric fluid and in breath in 50 patients of aluminium phosphide poisoning. The test was 100% positive with gastric fluid but was positive in 50% patients in breath. The test is simple, reliable and sensitive method to detect phosphine (PH3) for bed side diagnosis of aluminium phosphide (AIP) ingestion.

Estudo do pH intragástrico com o uso da cimetidine por via oral, intramuscular e venosa / Intragastric pH study with the use of cimetidine by oral, intramuscular and venous routes

Em 50 pacientes determinou-se o pH do suco gástrico após doses seqüenciais de cimetidine, administrada por via intramuscular e intravenosa. Os valores obtidos demonstram que a droga, administrada por essas vias, näo atinge níveis terapêuticos, sendo a via intravenosa a única realmente eficaz. Esta eficácia, todavia, só é uniforme entre a segunda e a quarta horas após o estímulo. Dois pacientes näo tiveram seu pH intragástrico aumentado mesmo após o emprego de doses elevadas. A administraçäo a intervalos de seis horas produz com freqüência uma curva de pH em "S" e deve ser usada somente em casos selecionados, com monitoraçäo da acidez gástrica. O emprego a intervalos de quatro horas provavelmente oferece melhor proteçäo contra lesöes agudas da mucosa gástrica, pépticas ou näo pépticas

Correlaçäo entre os valores do pH e concentraçäo de glicose no líquido cefalorraquídeo e sangue arterial em pacientes pediátricos / Correlation between pH values and glicose concentration in cerebrospinal fluid and arterial blood in pediatric patients

Em 30 pacientes, submetidos à anestesia raquídica, para cirurgias eletivas, foi colhida, previamente, amostra de líquor, para a verificaçäo do pH e valor da glicose, além da amostra de sangue arterial, com idêntica finalidade. Também foi colhida amostra de suco gástrico, em todos os pacientes, a fim de se verificar o valor do pH. Os valores obtidos, para o pH e glicose do líquor e sangue arterial, foram agrupados em tabelas e submetidos à análise estatística, com base no teste "t" de Student, adotando-se, como nível de significância, p < 0,05. As médias globais foram para o líquor: pH = 7,38 (7,13 a 8,50) e glicose = 62,60 (42 a 89) mg%; para o sangue arterial: pH = 7,34 (7,20 a 7,42) e glicose = 86 (68) a 130) mg% e para o suco gástrico: pH = 2,03 (1 a 5). O valor médio da relaçäo glicorraquia/glicemia foi 0,73 (0,46 a 0,92). Considerando que, em pacientes pediátricos, os valores médios dos parâmetros estudados säo escassos na literatura médica, achamos importante um trabalho desta natureza