RESUMO
BACKGROUND/AIMS: The aims of this study were to investigate whether a broccoli sprout extract containing sulforaphane (BSES) inhibited the Helicobacter pylori infection density and exerted an antioxidative effect on gastric mucosal damage. METHODS: The enrolled subjects were randomized in a double-blinded manner into three groups. Finally, 33 H. pylori (+) BSES treatment subjects (group A), 28 H. pylori (+) placebo subjects (group B), and 28 H. pylori (-) BSES treatment subjects (group C) were studied. H. pylori infection density was indirectly quantified by a 13C-urea breath test (UBT), and the ammonia concentration in gastric juice aspirates was measured through gastroscopic examination. Malondialdehyde (MDA), an oxidative damage biomarker, and reduced glutathione (GSH), an antioxidant biomarker, were measured in the gastric mucosa by an enzyme-linked immunosorbent assay. RESULTS: BSES treatment did not significantly affect the UBT values or ammonia concentration in group A (p=0.634 and p=0.505, respectively). BSES treatment did significantly reduce mucosal MDA concentrations in group A (p<0.05) and group C (p<0.001), whereas the gastric mucosal GSH concentrations did not differ before and after treatment in any of the groups. CONCLUSIONS: BSES did not inhibit the H. pylori infection density. However, BSES prevented lipid peroxidation in the gastric mucosa and may play a cytoprotective role in H. pylori-induced gastritis.
Assuntos
Adulto , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Amônia/metabolismo , Antioxidantes/farmacologia , Biomarcadores/análise , Brassica/química , Testes Respiratórios , Método Duplo-Cego , Ensaio de Imunoadsorção Enzimática , Suco Gástrico/enzimologia , Mucosa Gástrica/efeitos dos fármacos , Glutationa/análise , Infecções por Helicobacter/tratamento farmacológico , Helicobacter pylori , Isotiocianatos/farmacologia , Peroxidação de Lipídeos/efeitos dos fármacos , Malondialdeído/análise , Extratos Vegetais/química , UreiaRESUMO
In the present study we measured the activities of the following enzymes: LDH (lactic dehydrogenase), beta-glucuronidase, acid maltase, phosphohexoseisomerase (PHI) and acid proteases in the gastric juice of patients with gastric cancer (n = 50) (Case Group), in endoscopically normal subjects (n = 50) and in subjects with different non tumor-like digestive pathologies (n = 55) (Control Groups). In the patients with gastric carcinoma we found a significant increase in LDH, beta-glucuronidase, PHI and acid maltase activities and a decreased activity of acid proteases. The results agree with previous findings from other workers. The variations of enzyme activities in gastric juice can help to differentiate between malignant and benign processes of the gastric mucosa.
Assuntos
Humanos , Masculino , Feminino , Adulto , Pessoa de Meia-Idade , Adenocarcinoma/diagnóstico , Ensaios Enzimáticos Clínicos , Neoplasias Gástricas/diagnóstico , Suco Gástrico/enzimologia , Idoso de 80 Anos ou mais , Análise de Variância , Estudos de Casos e Controles , Glucose-6-Fosfato Isomerase/metabolismo , Glicosídeo Hidrolases/metabolismo , L-Lactato Desidrogenase/metabolismoRESUMO
The synthetic peptides AC-Glu-Phe-Phe (NO2)-Arg-amide (peptide VP) and AC-Ile-Glu-Phe-Phe (NO2)-Arg-amide (peptide VIP) are more readily hydrolyzed by human pepsin in gastric juice of patients of gastritis than those of duodenal ulcer and normal subjects. The kinetic parameters suggest that S3 subsite of the enzyme plays a role in the elevation of enzyme activity in gastric disease.
Assuntos
Sequência de Aminoácidos , Sítios de Ligação , Úlcera Duodenal/enzimologia , Suco Gástrico/enzimologia , Gastrite/enzimologia , Humanos , Cinética , Dados de Sequência Molecular , Oligopeptídeos/metabolismo , Pepsina A/metabolismo , Valores de Referência , Especificidade por Substrato , Peptídeo Intestinal Vasoativo/metabolismoAssuntos
Animais , Gatos , Núcleo Caudado/fisiologia , Estimulação Elétrica , Feminino , Suco Gástrico/enzimologia , Masculino , Pepsina A/análiseRESUMO
The present experiment was planned to assess the role of vagus and adrenals in gastric secretion in the pylorus ligated rats. Vagotomy significantly reduced the volume of gastric juice but the reduction following adrenalectomy was not significant. Both vagotomy and adrenalectomy significantly reduced the free acid, total acid and pepsin contents of the gastric secretion, the reduction being more pronounced following vagotomy.