The acute effects of preoperative ozone theraphy on surgical wound healing

ABSTRACT PURPOSE: To investigate the effects of preoperative rectal ozone insufflation on surgical wound healing over the proinflammatory cytokines and histopathological changes. METHODS: Twenty one rabbits were divided into 3 groups. Sham, surgical wound, and ozone applied (6 sessions, every other day 70 µg/mL in 12 mL O2-O3 mixture rectally) surgical wound groups were created. TNF-alpha and IL-6 levels from all rabbits were studied at the basal, 24th hour, and 72nd hour. The histopathological examination was done by removing the surgical scar tissue at the end of 72nd hour. RESULTS: TNF-alfa and IL-6 levels were significantly lower compared to the control group, in the rabbits treated with ozone. The increase in angiogenesis, the decrease in the number of inflammatory cells, epidermal and dermal regeneration, better collagen deposition, and increased keratinisation in stratum corneum were observed in the histopathological examination. It was determined that the wound healing noticeably accelerated in the ozone group. CONCLUSION: Preoperative rectal ozone insufflation had a positive effect on surgical wound healing in acute period.

Tracheal Wall Thickening Is Associated with the Granulation Tissue Formation Around Silicone Stents in Patients with Post-Tuberculosis Tracheal Stenosis

PURPOSE: Tracheal restenosis due to excessive granulation tissue around a silicone stent requires repeated bronchoscopic interventions in patients with post-tuberculosis tracheal stenosis (PTTS). The current study was conducted to identify the risk factors for granulation tissue formation after silicone stenting in PTTS patients. MATERIALS AND METHODS: A retrospective study was conducted between January 1998 and December 2010. Forty-two PTTS patients with silicone stenting were selected. Clinical and radiological variables were retrospectively collected and analyzed. RESULTS: Tracheal restenosis due to granulation tissue formation were found in 20 patients (47.6%), and repeated bronchoscopic interventions were conducted. In multivariate analysis, tracheal wall thickness, measured on axial computed tomography scan, was independently associated with granulation tissue formation after silicone stenting. Furthermore, the degree of tracheal wall thickness was well correlated with the degree of granulation tissue formation. CONCLUSION: Tracheal wall thickening was associated with granulation tissue formation around silicone stents in patients with post-tuberculosis tracheal stenosis.

An endodontic sealer induces a pathological condition when associated with persistent tissue toxicity and presence of myofibroblasts

The aims of this study were to evaluate the ratio between inflammatory reactions induced by four endodontic sealers and the occurrence of fibrosis and the number of myofibroblasts with positivity to α-smooth-actin muscle (α-SMA). Polyethylene tubes were filled with a root canal sealer (Endofill, AH Plus, Acroseal and Epiphany) and inserted into 4 site at the dorsal region of 24 Wistar rats; 2 empty tubes (control) were grafted in 6 rats. After 7, 21, and 45 days, 8 animals were euthanized, providing 6 specimens per test group and 2 specimens from the control group. The fragments were subjected to histological processing and immunohistochemical analysis for anti α-SMA protein. All specimens, except those from the control group, presented severe inflammatory reaction on the 7th postoperative day, which also coincided with a large number of myofibroblasts. On the 21st and 45th days post-surgery, the inflammatory reaction induced by Endofill, AH Plus and Acroseal decreased significantly, which coincided with reduced presence of myofibroblasts and usual collagen deposition. In contrast, in the group filled with Epiphany, significant inflammatory cell infiltrate was present in all analyzed periods. The persistence of an inflammatory reaction induced by endodontic sealer may also induce the development of fibrosis in combination with presence of myofibroblasts.

O objetivo deste estudo foi avaliar a relação entre reação inflamatória induzida por quatro cimentos endodônticos e a presença de fibrose e quantidade de miofibroblastos que apresentam positividade para α-SMA. Tubos de polietileno foram preenchidos com o cimento (I: Endofill; II: AH Plus; III: Acroseal; IV: Epiphany) e inseridos em 4 regiões do dorso de 24 ratos Wistar, enquanto 2 tubos vazios (V - controle) foram inseridos em 6 ratos. Após 7, 21 e 45 dias, oito animais foram sacrificados obtendo 6 indivíduos por grupo e 2 para o grupo controle. Os fragmentos foram submetidos ao processamento histológico e à análise imuno-histoquímica para a proteína anti-α-SMA. Todos os grupos, exceto o controle, demonstraram notável reação inflamatória no 7º dia pós-operatório, que também coincidiu com uma grande quantidade de miofibroblastos. No 21º e 45º dia pós-operatório, a reação inflamatória induzida pelo Endofill, AH Plus e Acroseal diminuiu significativamente, o que coincidiu com reduzida presença de miofibroblastos e deposição de colágeno normal. Em contraste, no grupo Epiphany, infiltrado inflamatório significativo esteve presente em todos os períodos analisados. A persistência do infiltrado inflamatório induzido por cimento endodôntico pode também provocar uma fibrose associada com a presença de miofibroblastos.

Subcutaneous connective tissue response to primary root canal filling materials

This study evaluated the response of the subcutaneous connective tissue of BALB/c mice to root filling materials indicated for primary teeth: zinc oxide/eugenol cement (ZOE), Calen paste thickened with zinc oxide (Calen/ZO) and Sealapex sealer. The mice (n=102) received polyethylene tube implants with the materials, thereby forming 11 groups, as follows: I, II, III: Calen/ZO for 7, 21 and 63 days, respectively; IV, V, VI: Sealapex for 7, 21 and 63 days, respectively; VII, VIII, IX: ZOE for 7, 21 and 63 days, respectively; X and XI: empty tube for 7 and 21 days, respectively. The biopsied tissues were submitted to histological analysis (descriptive analysis and semi-quantitative analysis using a scoring system for collagen fiber formation, tissue thickness and inflammatory infiltrate). A quantitative analysis was performed by measuring the area and thickness of the granulomatous reactionary tissue (GRT). Data were analyzed by Kruskal-Wallis, ANOVA and Tukey's post-hoc tests (?=0.05). There was no significant difference (p>0.05) among the materials with respect to collagen fiber formation or GRT thickness. However, Calen/ZO produced the least severe inflammatory infiltrate (p<0.05). The area of the GRT was significantly smaller (p<0.05) for Calen/ZO and Sealapex. In conclusion, Calen/ZO presented the best tissue reaction, followed by Sealapex and ZOE.

Neste estudo avaliou-se a resposta do tecido conjuntivo subcutâneo de camundongos BALB/c a materiais obturadores de canal radicular de dentes decíduos: óxido de zinco/eugenol (OZE), pasta Calen® espessada com óxido de zinco (Calen/OZ) e cimento Sealapex®. Os camundongos (n=102) receberam implantes de tubos de polietileno??? e foram divididos em grupos: I, II, III - Calen/OZ (7, 21 e 63 dias, respectivamente); IV, V, VI - Sealapex (7, 21 e 63 dias, respectivamente); VII, VIII, IX - OZE (7, 21 e 63 dias, respectivamente); X, XI - tubo vazio (7 e 21 dias, respectivamente). Os tecidos foram submetidos ao processamento e análise histopatológica descritiva e por meio de escores do fibrosamento, espessura tecidual e infiltrado inflamatório. Para a análise quantitativa mensurou-se a área e a espessura do tecido granulomatoso reacional (TGR). Os resultados foram analisados pelos testes de Kruskal-Wallis, ANOVA e pós-teste de Tukey (?=0,05). Não houve diferença significante (p>0,05) entre os materiais, com relação ao fibrosamento e à espessura do TGR. Contudo, Calen/OZ apresentou infiltrado inflamatório de menor intensidade (p<0,05). A área do TGR foi menor (p<0,05) para Calen/OZ e Sealapex. Pôde-se concluir que Calen/OZ foi o material que apresentou a melhor compatibilidade tecidual, seguido pelos cimentos Sealapex e OZE.

Evaluation of subcutaneous and alveolar implantation surgical sites in the study of the biological properties of root-end filling endodontic materials

OBJECTIVE: The aim of this study was to compare two methodologies used in the evaluation of tissue response to root-end filling materials in rats. MATERIAL AND METHODS: Forty rats were divided into 4 groups: in Groups I and II (control groups), empty polyethylene tubes were implanted in the extraction site and in the subcutaneous tissue, respectively; in Groups III and IV, polyethylene tubes filled with ProRoot MTA were implanted in the extraction site and in the subcutaneous tissue, respectively. The animals were killed 7 and 30 days after tube implantation, and the hemi-maxillas and the capsular subcutaneous tissue, both with the tubes, were removed. Specimens were processed and evaluated histomorphologicaly under light microscopy. The scores obtained were analyzed statistically by the Kruskal-Wallis test (p<0.05). RESULTS: There were no statistically significant differences between the implantation methods (p=0.78033, p=0.72039). It was observed that the 30-day groups presented a more mature healing process due to smaller number of inflammatory cells. CONCLUSIONS: The present study showed no differences in tissue responses as far as the implantation site and the studied period were concerned. Alveolar socket implantation methodology represents an interesting method in the study of the biological properties of root-end filling endodontic materials due to the opportunity to evaluate bone tissue response.

Histological analysis of the association between formocresol and endotoxin in the subcutaneous tissue of mice

This study performed a histological analysis of the effect of formocresol associated to endotoxin (LPS) in the subcutaneous connective tissue of mice. Ninety mice were randomly assigned to 3 groups (n=30). Each animal received one plastic tube implant containing endotoxin solution (10 mg/mL), formocresol (original formula) or a mixture of endotoxin and formocresol. The endotoxin and formocresol groups served as controls. The periods of analysis were 7, 15 and 30 days. At each experimental period, tissue samples were collected and submitted to routine processing for histological analysis. Endotoxin and formocresol produced necrosis and chronic inflammation at 7 and 15 days. At 30 days, the endotoxin group showed no necrosis, while in the formocresol group necrosis persisted. The formocresol-endotoxin association produced necrosis and chronic inflammation in the same way as observed with formocresol at all experimental periods. In conclusion, formocresol seems not to be able to inactive the toxic effects of endotoxin in connective tissues.

O objetivo deste estudo foi avaliar histologicamente o efeito da associação do formocresol com endotoxina (LPS) em tecido conjuntivo de camundongos. Noventa camundongos foram divididos em três grupos de 30 camundongos cada. Cada camundongo recebeu um implante subcutâneo de tubo plástico contendo solução de endotoxina (10 mg/ml), formocresol (fórmula original), ou uma mistura de formocresol com endotoxina. Os grupos da endotoxina e formocresol foram considerados grupos controle. Os períodos de análise foram 7, 15 e 30 dias. Após os períodos experimentais, os tecidos foram removidos e submetidos a processamento histológico. Os resultados obtidos indicam que a endotoxina e o formocresol produzem necrose e inflamação tecidual crônica aos 7 e 15 dias e aos 30 dias o grupo da endotoxina não mostrava necrose e no grupo do formocresol a necrose persistiu. A combinação formocresol e endotoxina mostrou necrose e inflamação crônica com resultados semelhantes ao do grupo formocresol para todos os períodos experimentais. Pode-se concluir que o formocresol parece não ser capaz de inativar os efeitos tóxicos da endotoxina.

Wound healing activity of alcoholic extract of Kaempferia galanga in Wistar rats.

The wound healing effect of alcoholic extract of Kaempferia galanga (K. galanga) and its effect in dexamethasone suppressed wound healing was studied in Wistar rats. Three wound models viz. incision, excision and dead space wounds were used in this study. The parameters studied were breaking strength in case of incision wounds, epithelialization and wound contraction in case of excision wound and granulation tissue dry weight, breaking strength and hydroxyproline content in case of dead space wound. The dexamethasone treated group showed a significant (P < 0.001) reduction in the wound breaking strength when compared to control group in incision type of wound model. Coadministration of K. galanga with dexamethasone had significantly (P < 0.001) increased the breaking strength of dexamethasone treated group. In excision wound model, the percentage of the wound contraction was significantly (P < 0.05) increased by K. galanga only on 16th day and also it reversed the dexamethasone suppressed wound contraction on the 16 day. K. galanga significantly (P < 0.001) reduced the time required for epithelialization and reversed the epithelialization delaying effect of dexamethasone significantly (P < 0.001).

Morphological analysis of three wound-cleaning processes on potentially contamined wounds in rats / Análise morfológica de três tipos de limpeza em feridas cutâneas potencialmente infectadas em ratos

PURPOSE: To evaluate the inflammatory response of potentially infected wounds treated with isotonic saline solution, chlorhexidine and PVP-I, seven days after surgery. METHODS: Thirty-two male rats were used, divided into 4 groups. All animals had their surgical wounds infected with a standard bacterial inoculum. Control group (A): animals had their surgical wounds sutured without any kind of cleaning. Saline solution group (B): animals had their wounds cleaned with saline solution. Chlorhexidine group (C): animals had their wounds cleaned with chlorhexidine. PVP-I group (D): animals had their wounds cleaned with PVP-I. Seven days after surgery, all the animals had their skin submitted to microscopic and macroscopic evaluation. RESULTS: Edema was found on all histological slices analyzed, as well as vascular proliferation and congestion. Groups A and D showed presence of mild neutrophilic infiltrate, and moderate lymphocytic and macrophage infiltrate. Group B showed severe neutrophilic, macrophage, and lymphocytic infiltrate. Group C showed moderate neutrophilic, macrophage, and lymphocytic infiltrate. CONCLUSION: Group D was the group which showed inflammatory infiltrate most similar to the group that was not submitted to treatment.

OBJETIVO: Avaliar a resposta inflamatória da limpeza de feridas potencialmente infectadas tratadas com PVP-I, clorexidina e solução salina isotônica, no sétimo dia de pós-operatório, em ratos Wistar. MÉTODOS: Utilizou-se 32 ratos distribuídos em quatro grupos. Os animais tiveram suas feridas operatórias infectadas com um inóculo bacteriano padrão. Grupo controle (A): os animais tiveram suas feridas operatórias suturadas sem a limpeza com qualquer substância. Grupo solução salina (B): os animais tiveram suas feridas operatórias lavadas com solução salina. Grupo Clorexidina (C): os animais tiveram suas feridas operatórias lavadas com clorexidina. Grupo PVP-I (D): os animais tiveram suas feridas operatórias lavadas com PVP-I. Após sete dias foi realizada a avaliação morfológica da pele. RESULTADOS: Em todos cortes histológicos observou-se a presença de edema, proliferação e congestão vascular. O grupo A e D mostraram a presença de um infiltrado neutrofílico discreto, macrofágico e linfocítico moderados. O grupo B, um infiltrado neutrofílico, macrofágico e linfocítico severos. O grupo C, um infiltrado de neutrófilos, macrófagos e linfócitos em moderada quantidade. CONCLUSÃO: O grupo D foi o grupo que apresentou o infiltrado inflamatório mais semelhante com o grupo que não recebeu nenhum tratamento.

Tissue effects of fine needle aspiration on salivary gland tumours.

Fine needle aspiration cytology (FNAC) is a widely used diagnostic procedure to evaluate lesions in the salivary glands. Though regarded as a safe and reliable procedure ,a variety of histological changes following FNAC have been reported. We studied the FNAC related tissue changes in 50 neoplasms of the salivary gland and the impact on subsequent histological evaluation. FNAC induced changes were found in 34% (17/50) with needle tract (10/50)and infarction (7/50) as the commonest changes .These changes did not interfere with the subsequent histological evaluation of the tumours. We conclude that knowledge of a previous FNAC and awareness of its effects on histology may avoid potential misdiagnosis, though the incidence of significant changes is small.

Rat subcutaneous tissue response to macrogranular porous anorganic bovine bone graft

The ideal bone graft must present biocompatibility, osteoconductive and osteoinductive properties, resistance and plasticity. Xenogenic grafts of bovine cancellous bone origin are particularly interesting due to their biologically designed porous structure that enhance both cellular and vascular invasion. The purpose of this study was to evaluate the tissue response induced by bovine macrogranular porous anorganic bone implanted in rat subcutaneous tissue. Forty rats were assigned to 2 groups, as follows: the control group received empty collagen capsules and the test group received subcutaneous implants of the test material. Samples were collected after 10, 20, 30 and 60 days and processed histologically. Histological analysis showed at 10 days a granulomatous inflammatory infiltrate, rich in multinucleated giant cells and free of lymphocytes or plasma cells, similarly to mineralized allograft implanted in rat subcutaneous. In later periods, there was a significant decrease in the inflammatory infiltrate and an increase in fibrosis around graft particles. In conclusion, the test material induced a foreign body-type granuloma with subsequent fibrosis around the graft particles implanted in rat subcutaneous and did not elicit any immune response, thus being considered biocompatible.

O enxerto ósseo ideal deve possuir características como biocompatibilidade, capacidade osteocondutora, osteoindutora, resistência e plasticidade. Dentre os implantes xenogênicos de origem bovina, os produzidos com o osso esponjoso revestem-se de particular interesse devido a sua arquitetura constituída de poros biologicamente desenhados que favorecem a invasão celular e vascular até o centro do defeito. O objetivo deste estudo foi avaliar a resposta tecidual ao material de osso inorgânico medular bovino macrogranular. Quarenta ratos foram divididos em 2 grupos (n=20): o grupo controle recebeu cápsulas de colágeno vazias, e o grupo experimental recebeu implante subcutâneo do material teste. As amostras foram coletadas após 10, 20, 30 e 60 dias de implantação e processadas histotecnicamente. A análise histológica mostrou aos 10 dias pós-cirúrgicos que o infiltrado inflamatório era do tipo granulomatoso rico em células gigantes multinucleadas, mas livre de linfócitos ou plasmócitos, quadro similar ao observado para aloenxertos mineralizados implantados em subcutâneo de ratos. Com o avançar do tempo experimental houve significante diminuição do infiltrado inflamatório inicial concomitantemente ao aumento no grau de fibrosamento ao redor das partículas implantadas. Concluiu-se que o material de enxerto testado em tecido conjuntivo subcutâneo de ratos induziu um granuloma tipo corpo estranho e fibrose ao redor das partículas implantadas, resposta semelhante do mesmo tecido aos aloenxertos mineralizados, e não desencadeou nenhuma resposta imune, sendo portanto biocompatível.

Histometric study of socket healing after tooth extraction in rats treated with diclofenac

The purpose of the present study was to investigate if diclofenac administration interferes with the time course of alveolar wound healing in rats. Forty-two Wistar rats were used, 21 rats received 10 mg/kg/day of diclofenac one day before and 4 days after extraction of the right maxillary incisors and 21 rats received saline. The animals were sacrificed 7, 14 and 21 days after tooth extraction. Progressive new bone formation and a decrease in the volume fraction of blood clot and connective tissue from 1 to 3 weeks after tooth extraction was quantified using the histometric point-counting method. Diclofenac treatment caused a significant delay in new bone formation in association with an impairment of blood clot remission/organization.

Comparative study of tissue response to polyglecaprone 25, polyglactin 910 and polytetrafluorethylene suture materials in rats

The authors evaluated the biocompatibility of three suture materials, polyglecaprone 25, polyglactin 910 and polytetrafluorethylene, implanted into subcutaneous tissue in the dorsal region of 20 Wistar albinus rats. After periods of 2, 7, 14 and 21 days, the rats were sacrificed and the specimens were processed for routine histotechnical analysis and stained with hematoxylin and eosin. The rate of fibrosis, angioblastic and fibroblastic proliferation, and also the intensity of inflammation were observed with the optic microscope. The results showed that polyglecaprone 25 suture material induced a mild inflammatory reaction, followed by polyglactin 910 and polytetrafluorethylene, respectively. Such biological behavior must be considered during the selection of the suture material to be used in oral surgery.

Morfologia de las celulas en los tumores mixoides / Morphology of the cells in myxoid tumors

Describimos un estudio morfológico de las células en tumores mixoides o con áreas mixoides: fibrosarcomna mixoide, mixoma, liposarcoma mixoide, rabdomiosarcoma embrionario, condroma y condrosarcoma, leiomiosarcoma mixoide, Schwannoma y odontoameloblastoma; y se las compara con los fibroblastos del tejido conectivo reticular del cordón umbilical, del mesénquima embrionario, y del tejido conectivo laxo en procesos inflamatorios. Los métodos utilizados son: H-E, PAS, tricrómico de Masson y la técnica panóptica de Del Río Hortega. Los resultados con la técnica panóptica revelan fibroblastos bipolares con largas prolongaciones. En el mixoma y fibrosarcoma mixoide son estrellados con múltiples prolongaciones y con secreción mucinosa citoplasmática contenida en ampollas o bochas que se deslizan por las prolongaciones hasta resolverse en sus extremos o volcarse al espacio intercelular. En el lipoblastoma mixoide el lipoblasto es también estrellado con múltiples prolongaciones y gotas de lípidos. Los rabdomioblastos son raquetoides con estriaciones transversales; los condroblastos poseen amplio citoplasma con procesos cortos. Los leiomioblastos tienen citoplasma acintado, núcleos en habano y miofibrillas. Las células de Schwann tienen largas y finas prolongaciones opositopolares; los odontoblastos muestran amplio citoplasma con numerosas prolongaciones orientadas hacia la dentina. Estos detalles morfológicos ayudan al diagnóstico diferencial de estos tumores complementados con la inmunocitoquímica

Dystrophic calcification in the neck--a case report.

One 55 years aged male person was admitted with progressive painless neck swelling with multiple discharging sinuses. His X-ray of the neck showed a dense radio-opaque shadow & serum calcium level was 2 mmol/l. On neck exploration, an irregular calculus was revealed; hooking around the left carotid sheath. Histopathology confirmed the clinical diagnosis of tuberculosis. Anti-TB chemotherapy for six months cured him.

Arachnoid granulation affected by subarachnoid hemorrhage

Por microscopia óptica foram estudados os componentes fibrosos das granulaçöes aracnódes de indivíduos acometidos por hemorragia subaracnóide de tipo moderado ou severo. Os eritrócitos estavam presentes nos canais delimitados por feixes de fibras elásticas, colágenas e células aracnóides. Os canais tortuosos e intercomunicantes eram observados desde o pediculo até a cápsula fibrosa da granulaçäo aracnóide. O principal trajeto dos eritrócitos e do líquido cáfalo-raquidiano ocorria no interior do pedículo e centro da granulaçäo aracnóide. Na hemorragia severa, os compoentes fibro-musculares estavam desorganizados e os canais extra-celulares aumentados. A presença de células com grandes núcleos, observada no material hemorrágico, é sugestiva de transformaçöes das células aracnóides em células fagocitárias, para promover a digestäo intracelular dos eritrócitos

Human fibroblasts in idiopathic retroperitoneal fibrosis express HLA-DR antigens

Idiopathic retroperitoneal fibrosis (IRF) is a rare human disease characterized by non-neoplastic fibroblastic proliferation associated with chronic inflammatory cells; its pathogenesis is obscure. We undertook an immunohistochemical study for the expression of HLA-DR antigens and other immune-related markers by retroperitoneal proliferating fibroblasts and inflammatory cells from 2 IRF patients. Patterns of immunoreactivity were compared with those expressed by human nodular fasciitis (NF) and granulation tissue. In IRF, most fibroblasts immunostained strongly for HLA-DR antigens, whereas fibroblasts in NF and granulation tissue did, not immunostain at all. The fibroblasts did not immunostain for interleukin 2 receptor, C3b receptor, CD-4, CD-8, or Leu-M1 in any of the tissue studied. Most macrophages and lymphocytes in IRF and NF immunostained Strangly for HLA-DR antigens. In IRF, the CD-4 and CD-8 immunostained T-lymphocytes appeared equally distributed. The expression of HLA-DR antigens by fibroblasts in IRF indicates that this rare disease may indeed be an immune-associated hypersensitivity disorder.

Tecido de granulaçäo de ratos em condiçöes normais e sob açäo de drogas inflamatórias: estudo histológico e histofotométrico / Granulation tissue of rats in normal condictions and under the action of inflammatory drugs: histological and histophotometric study

O presente trabalho teve como uma de suas finalidades, acompanhar a síntese de colágeno e glicosaminoglicans (mucopolissacarídeos ácidos) no tecido de granulaçäo, induzido artificialmente em ratos, desde uma fase precoce até sua maturaçäo. Por outro lado, objetivou-se estudar os efeitos de drogas antiinflamatórias, com princípios ativos diferentes, na evoluçäo deste mesmo tecido, comparando-a com aquela observada em animais sob condiçöes normais. Os resultados obtidos, de acordo com as doses empregadas, demonstraram que a dexametasona 21-fosfato é uma potente inibidora da síntese de mucopolissacarídeos ácidos e de colágeno, o mesmo acontecendo com a fenilbutazona, esta, porém, inibindo tal síntese em menor grau. Paralelamente, a tripsina pareceu interferir na proliferaçäo do tecido de granulaçäo de uma maneira positiva, quantitativa como qualitativamente. A partir desses resultados, pode-se concluir que o corticosteróide sintético (dexametasona 21-fosfato), assim como a fenilbutazona, nas doses administradas, constituem-se em grandes recursos terapêuticos quando houver necessidade de uma inibiçäo da proliferaçäo do tecido de granulaçäo. Ao contrário, o antiinflamatório de origem enzimática (tripsina) estaria indicado em processos inflamatórios nos quais uma síntese mais rápida e organizada do tecido de granulaçäo for de primordial importância

Estudo histológico do tecido de granulaçäo de feridas cutâneas de ratos hipotireoides e parotidectomizados / Histologic study of cutaneous wound's granulation tissue of hypothyroid and parotidectomized rats

Os autores procederam ao estudo histológico do processo de reparo de feridas cutâneas dorso-lombares de ratos submetidos à administraçäo diária de 25 mg de 6-propil-2-tiouracil e de ratos parotidectomizados. Os animais foram sacrificados, 4, 8 e 12 dias após a realizaçäo da ferida dorso-lombar tendo sido as peças obtidas por dissecaçäo incluídas em parafina pelo método rotineiro de uma vez processadas, foram coradas pela hematoxilinma-eosina, pelo tricrômico de Masson e pelo método de Van Gieson. A análise dos resultados, permitiu verificar que, em relaçäo aos animais controle, houve atraso na formaçäo e maturaçäo do tecido de granulaçäo dos animais tratados com 6-propil-2-tiouracil e antecipaçäo na formaçäo e maturaçäo do tecido de granulaçäo nos animais paratidectomizados