Ventral abdominal wall defect correction in rats with contaminated meshes

ABSTRACT PURPOSE: To investigate whether there is a difference between Marlex(r) and Dynamesh PP-light Marlex(r) meshes, in the abdominal wall defect correction, on rats in contaminated surgical site. METHODS: Twenty-eight Wistar rats were divided into two groups of 14, and four subgroups of seven animals. All subgroups underwent similar surgical procedure. One group received the mesh Marlex(r) and the other Dynamesh PP-light(r) for correction of the defect. Before implanting, the meshes went through a contamination process, on which was used standard solution containing 10 UFC of Escherichia coli. Fragments of the animal's abdominal wall received macroscopic, microscopic and microbiological analysis. RESULTS: There was no statistical significance in the analysis of macroscopic variables. Accentuated inflammatory process was shown in all subgroups. The foreign body type reaction was mild in all subgroups, except Dynamesh(r)-14, which was moderate with no statistical significance. The microbiological analysis of the meshes was also similar between the subgroups. CONCLUSION: There was no difference between the meshes of Marlex(r) and Dynamesh PP-light(r) in the ventral abdominal wall defect correction on rats in contaminated surgical site.

Chronic postoperative wound infection caused by Myocobacterium fortuitum complex.

Mycobacterium fortuitum in a rapidly growing atypical mycobacteia, sometimes associated with nosocomial infections in human. These infections are often difficult to identify; and treat even after indentification. We report here a case of chronic post operative wound infection due to M. fortuitum.

Conservative management of mesh site infection in ventral hernia repair

Mesh Hernioplasty is the preferred surgical procedure for abdominal wall hernias and infection remains one of the most common complications of this technique. In some patients the mesh may need removal to overcome infection, where as others may be salvaged by conservative treatment. This study was conducted to assess the outcome of conservative management for mesh site infection in abdominal wall hernia repairs. This study was carried out in Ayub Teaching Hospital Abbottabad Pakistan from Jan 2006 to Dec 2007. Thirteen consecutive cases were included, who developed mesh site infection after abdominal wall hernia repair. Pus or purulent fluid was sent for culture and sensitivity. All patients were treated by intravenous antibiotics and local wound care. Treatment was taken as successful when there was complete resolution of infection and healing of the wound. There were 7 inguinal [53.84%], 4 para-umbilical [30.76%] and 2 incisional hernias [15.38%]. Eight patients were males [61.53%] and 5 females [38.46%]. Median age of the patients was 40 years [range 28 to 52 years]. Staphylococcus aureus was the most commonly found organism causing infection in 8 patients, [76.9%]. Mean hospital stay was 22 days [range 18-26 days]. All cases were effectively treated conservatively without removing the mesh. Polypropylene mesh was used in all of these cases. Conservative management is likely to be successful in mesh site infection in abdominal wall hernia repairs

Infection on the meshes implantation area in the abdominal wall of rats with induced bacterial peritonitis

OBJETIVO: Avaliar a incidência do crescimento bacteriano em telas de polipropileno implantadas na parede abdominal de ratos, após a indução de peritonite bacteriana.MÉTODOS: Utilizaram-se 36 animais alocados em dois grupos: grupo B, experimento (n=18) e grupo S, controle (n=18). Os ratos foram submetidos ao implante de telas de polipropileno na parede abdominal, no espaço pré-peritoneal. Em seguida, nos animais do grupo experimento, procedeu-se à indução de peritonite pela inoculação na cavidade peritoneal de solução padronizada de Escherichia coli. Nos animais do grupo controle procedeu-se à inoculação de solução fisiológica. Os animais de ambos os grupos foram realocados em três subgrupos de seis animais e acompanhados até as reoperações para avaliação dos sítios de implantação, coleta das telas para culturas, avaliação da cavidade e lavados peritoneais para culturas. As reoperações ocorreram com 24, 48 e 72 horas.RESULTADOS: Todos os animais do grupo experimento apresentaram quadro de peritonite. As culturas das telas retiradas dos sítios de implantação mostraram-se positivas em 83% dos animais quando o momento das avaliações foi de 24 horas, diminuindo para 33% em 48 horas e 17% em 72 horas, globalmente foi de 44%. Nos animais do grupo controle não houve nenhum caso de cultura positiva, tanto nas telas quanto nos lavados peritoneais. CONCLUSÕES: O modelo experimental utilizado foi efetivo produzindo 100% de peritonites. A incidência de crescimento bacteriano nas telas de polipropileno implantadas foi de 83% com 24 horas, decrescendo com o passar do tempo.