Biochemical effect of soybean and insecticide on tissue of rat, measured by infrared spectroscopy

The infrared spectroscopic techniques were used for the elucidation of the alteration in rat tissues, caused by gardona [tetrachloroviphose] insecticide and soybean feeding. The analysis of the infrared spectra of the tissue samples provided strong evidence that soybean causes considerahle increase in cysteic acid and sulfoxide contents of the liver. Soybean also caused a decrease in lipid content of the kidney, spleen and intestine, this was opposed by an increase in their proteins. The effect of soybean and insecticide was similar to the effect of soybean alone. On the other hand, the effect of the insecticide alone on the above mentioned tissues was undetectable. The lung tissue was neither affected by soybean nor by the insecticide

Short term toxicity studies on Dursban and Gardona in mice

The toxicity and the in vivo cholinesterase activity of the two insecticides dursban and gradona in mice have been investigated following different routes of application. Dursban inhibited both erythrocyte and plasma cholinesterase but gardona had no effect on the activity of erythrocyte enzyme. These studies were carried out by pure insecticides synthesized in our laboratory