Scanning electron microscopy approach for evaluation of hair dyed with Lawsonia inermis powder: in vitro Study / Método de microscopía electrónica de barrido para la evaluación de cabello teñido con extracto de Lawsonia inermis: estudio in vitro

During aging, usually graying of the hair occurs as a result of oxidative stress. Driven by social acceptance and self-perception of the exterior appearance, both men and women rely on hair dyeing products, in order to mask the graying hair. At the same time, a frequent use of synthetic products and treatment can damage the hair shaft; for this reason, this study aimed to evaluate the morphological effect of the herbal dye derived from Lawsonia inermis (henna), on hair. Dyed hairs were evaluated by means of SEM. Subsequently, they were compared, qualitatively and quantitatively, with undyed hairs. Results showed a positive impact on the cuticula pattern and on the diameters of the examined samples, after henna application. Different results, about the degree and the type of morphological changes occurring on pigmented hairs, may depend on the phenotype and on the health condition of hair, before dye treatment.

Durante el envejecimiento, generalmente se produce el envejecimiento del cabello como resultado del estrés oxidativo. Motivados por la aceptación social y la autopercepción de la apariencia, tanto hombres como mujeres confían en productos para teñir el cabello para enmascarar las canas. Al mismo tiempo, el uso frecuente de productos y tratamientos sintéticos puede dañar el tallo del cabello. Por esta razón, este estudio tuvo como objetivo evaluar el efecto morfológico del tinte derivado de Lawsonia inermis (henna) en el cabello. Los cabellos teñidos se evaluaron mediante SEM. Posteriormente, se compararon, cualitativa y cuantitativamente, con cabellos sin teñir. Los resultados mostraron un impacto positivo en el patrón de la cutícula y en los diámetros de las muestras examinadas, después de la aplicación de henna. Los diferentes resultados, sobre el grado y el tipo de cambios morfológicos que ocurren en los cabellos pigmentados, pueden depender del fenotipo y del estado de salud del cabello, antes del tratamiento con tinte.

A case of hair dye-induced contact anaphylaxis

Anaphylaxis is a sudden-onset life-threatening systemic hypersensitivity reaction. Allergens, such as foods, stinging insect venoms, and drugs, are the globally important causative factors for anaphylaxis. Para-phenylenediamine (PPD), an aromatic amine, is a well-known hair dye component that can act as a skin irritant and/or a skin sensitizer. As an allergen, PPD can induce various reactions; the most common being contact dermatitis, a delayed-type hypersensitivity reaction. Anaphylaxis or other immediate hypersensitivity reactions by hair dye contact is extremely rare, with only a few cases reported worldwide. Here, we report a case of a 63-year-old female who presented to the Emergency Department with dyspnea, rash, vomiting, and diarrhea within minutes after using a hair dye product containing PPD. Her past medical history includes urticaria of unknown cause. Her total IgE antibody level was increased to 630 kU/L. Skin prick and patch tests with the hair dye she applied at the time of anaphylaxis demonstrated an immediate reaction. An additional patch test with 25 common contact allergens showed positive reaction to PPD. This is the first case report of hair dye-induced contact anaphylaxis presenting sensitization to PPD in Korea.

Two Cases of Pigmented Contact Dermatitis Caused by Pure Henna Hair Dyes

No abstract available.

P-Phenylenediamine Hair Dye Allergy and Its Clinical Characteristics

BACKGROUND: P-phenylenediamine (PPD) has been identified as the most frequent contact sensitizer of hair dye and the clinical characteristics of hair dye contact allergy (HDCA) are diverse. OBJECTIVE: The purpose of this study was to identify the clinical characteristics of HDCA and to assess the relationships between HDCA, exposure time to PPD and PPD positivity. METHODS: We analyzed 105 patients with patch test-confirmed hair dye allergy who presented between July 2009 and March 2015. Clinical symptoms, signs, associated skin diseases, involved ACD area, and patterns of hair dye use were obtained by reviewing medical records and by interview. RESULTS: HDCA was more common in women and in individuals aged more than 50 years. Pruritus was the most common symptom; erythematous macules and patches were the most frequently observed clinical signs. The most common site of HDCA was the face and non-specific eczema and urticaria were frequently observed with HDCA. Exposure time to hair dye, represented as frequency and duration, showed a positive correlation with the area affected by hair dye allergy (p < 0.001). Hair dye allergy was identified in most patients (80%) before diagnosis by patch test. However, only 28.6% of all patients stopped using hair dye, even after the diagnosis was confirmed. CONCLUSION: The extent of hair dye allergy involvement was related to exposure time to hair dye. For effective treatment of HDCA, it is important to properly educate patients with HDCA about the clinical manifestations of HDCA and to keep away from allergens.

Influence of different cosmetic vehicles in mechanical and physical properties of hair treated with oxidative hair dyes

ABSTRACT Hair care products play a significant role in the cosmetic market and aim at improving hair brightness, breakage resistance, and color change. In this study, we analyzed the possibility of the formulation of oxidative dyes in different vehicles impacting the hair's both mechanical and physical properties. Light brown and light blond dyes were prepared using eight different vehicles. Among these, four vehicles were emulsifying agents and four gelling agents. Each formulation was applied to two types of virgin Caucasian hair (light blond and dark brown). Physical, chemical, and organoleptic properties of each formulation were assessed, as well as changes in hair parameters after oxidative dyeing, such as staining intensity, brightness, and breaking strength. The parameters of color and brightness differed in some formulations, but the hair type also responded differently. Brightness parameter was increased in dark brown hair colored with both dyes, whereas light blond hair showed the opposite result. Regarding the breaking strength, there were no significant differences between the two kinds of tresses. Cosmetic formulations should adjust the consumer desired effects (e.g. color change) in order to present minimal drawbacks (e.g. decrease of hair brightness and strength). Thus, the study of different vehicles is important when establishing the best oxidative dye formulation.

Analysis of the Results from the Patch Test to Para-Phenylenediamine in the TRUE Test in Patients with a Hair Dye Contact Allergy

BACKGROUND: Para-phenylenediamine (PPD) is the primary patch test screening agent for hair dye contact allergy (HDCA). However, no recent studies have been published that describe the results of reactions to patch tests using PPD and hair dyes in Korea. OBJECTIVE: To analyze the results of the patch tests to PPD using the thin-layer rapid use epicutaneous (TRUE) Test system in patients with HDCA and to investigate patients' awareness that hair dyes contains allergens, which cause the development of HDCA. METHODS: Eighty-four patients with suspected HDCA (32 men and 52 women) underwent patch testing using the TRUE Test system and their own hair dyes. The patients' demographic data, behavior associated with hair dyeing, and clinical manifestations of HDCA were examined retrospectively. RESULTS: Positive patch-test reactions to hair dyes occurred in 53.6% of patients who used hair dyes, and they were diagnosed with HDCA. Although there was a significant correlation between HDCA and PPD sensitization (p=0.001), only 40% of the patients with HDCA showed positive reactions to PPD. Of the 45 patients diagnosed with HDCA, only 7 (15.6%) were aware that their hair dyes contained allergens that caused HDCA. CONCLUSION: This study's findings show that PPD is an effective HDCA marker. However, we propose that investigations on hair dye components other than PPD should be conducted to develop and validate additional predictive HDCA markers.

Hair cosmetics.

The hair cosmetic industry has undergone a revolutionary change over the last two decades. The focus has dramatically veered from merely cleaning to repair, increasing the tensile strength, reducing oxidative damage, and stimulating growth. Newer shorter procedures to make hair look naturally more lustrous, smooth, and manageable have evolved. Specialized grooming products have been formulated to cleanse, calm, and condition the hair, and are tailored for different hair-types, for example, dry, dry-damaged, oily, colored, and gray hair. Other products are formulated to alter the color or structure of the hair shaft, for example, hair dyes, perming/relaxing. Hair sprays and waxes/gels, can alter the 'lift' of the hair-shaft. Although dermatologists are experts in managing scalp and hair diseases, the esthetic applications of newer cosmetic therapies still remain elusive. This article attempts to fill the lacunae in our knowledge of hair cosmetics and esthetic procedures relevant in today's rapidly changing beauty-enhancing industry, with special emphasis on the Indian scenario for chemical and 'natural' hair products.

The Pattern of Hair Dyeing in Koreans with Gray Hair

BACKGROUND: Hair graying is considered as a part of normal ageing process. Nonetheless, this process raises a significant cosmetic concern, especially among ethnic Korean elderly whose baseline hair color is black. For this reason, Korean elderly dye their hair with frequency despite the risk of dermatologic problems such as allergic contact dermatitis. OBJECTIVE: In this study, the authors investigate the prevalence and pattern of hair dyeing and its relation with scalp diseases in Korea. METHODS: Six hundred twenty subjects (330 men and 290 women) with graying hair were given a questionnaire survery and underwent a physical examination. RESULTS: Of the 620 total, 272 subjects (43.9%) dyed their hair. Hair dyeing was significantly more frequent among women than among men (p<0.001). Subjects from 50 to 69 years of age showed higher prevalence of hair dyeing when compared to either younger or older groups. Subjective self-assessment of the extent of hair graying was associated with increased prevalence of hair dyeing, that is, individuals who feel graying has advanced by more than 20% of the overall hair were much more likely to dye their hair (p<0.001). Hair dyeing did not correlate with either alopecia or scalp disease. CONCLUSION: Our survey has found that the prevalence of hair dyeing is higher among Korean women than men. People in their fifties and sixties and people with more than 20% extent of grayness were more likely to dye their hair than otherwise. Hair dyeing was not associated with any increase in the prevalence of scalp diseases.

Efficacy and Safety of Pueraria lobata Extract in Gray Hair Prevention: A Randomized, Double-Blind, Placebo-Controlled Study

BACKGROUND: Graying of hair-a sign of aging-raises cosmetic concerns. Individuals with gray hair often look older than others their age; therefore, some dye their hair for aesthetic purposes. However, hair colorants can induce many problems including skin irritation, allergic reaction and hair-breakage. OBJECTIVE: This randomized, double-blind clinical trial was performed in order to examine the effects of APHG-1001, a compound including an extract from Pueraria lobata, on graying hair. METHODS: A total of 44 female subjects were randomly treated with either APHG-1001 or placebo twice daily for 24 weeks. Using the phototrichogram analysis, a count of newly developed gray hair was estimated. Investigator assessment and subject self-assessment were also performed in order to evaluate the efficacy of the compound. RESULTS: The mean number of newly developed gray hair at 24 weeks was 6.3/cm2 in the APHG-1001 group and 11.4/cm2 in the placebo group; the difference was statistically significant (p<0.05). However, the investigator assessment and subject self-assessment did not show any significant change in the gross appearance of hair grayness by the end of the study. No severe adverse events in either group were observed. Moreover, the incidence of adverse events did not differ between the groups. CONCLUSION: This clinical trial revealed that APHG-1001, which contains an extract of P. lobata, could prevent the development of new gray hair without any remarkable adverse effects. Thus, it can be considered as a viable treatment option for the prevention of gray hair.

A Survey of the Awareness, Knowledge and Behavior of Hair Dye Use in a Korean Population with Gray Hair

BACKGROUND: Gray hair naturally develops in the process of human aging. Many people with gray hair periodically dye their hair. Hair dyeing products are widely used and they can cause adverse effects. Therefore, the user's knowledge and recognition about hair dyeing and related side effects are important. OBJECTIVE: The goal of this study was to lay the foundation for understanding, preventing and treating side effects caused by hair coloring products. METHODS: We conducted a questionnaire survey for adult males and females aged over 20 who had gray hair. A total of 500 subjects were included in this study and statistical analysis was performed. RESULTS: Large numbers of the people who had experience with hair dye (233 out of 319 people, 73.0%) did not know about the exact brand name of the hair dye product that they were using. Of 319 hair dye users, 23.8% (76 out of 319) people stated that they experienced side effects. Despite the occurrence of side effects from hair dyeing products, it seems they did not realize the seriousness of the side effects or the need for treatment. CONCLUSION: It is advisable to introduce a system that enables users to become aware of the ingredients and side effects of hair coloring products and give opportunities for users to become aware of the side effects of hair coloring through education, publicity and publication of an informational booklet.

Clinical and experimental study of acute intoxication as a result of ingestion of stone hair dye containing paraphenylenediamine

Hair dyes containing para-phenylenediamine were used in some communities for criminal purposes and more frequently for attempted suicide and sometimes accidentally ingested. The aim of the present work to analyze the various aspects of poisoning fatalities as a result of stone hair dye [SHD] ingestion through a retrospective study of fatalities investigated by Assiut Chemical Laboratory of Medico-legal Department, Ministry of Justice in four governorates in Upper Egypt and to detect the systemic effects on experimental animals as a result of its ingestion and if there is dose-effect relationship. The records of acute poisoning cases of hair dye ingestion investigated by Assiut Medicolegal Laboratory in the period from January 2002 to December 2009 were examined as regarding type of poison, pattern, incidence, sex, geographical distribution and mode of poisoning. The studying of the systemic effects on ingestion of hair dye was conducted on 30 albino rats divided into five groups each contain 6 animals. The first group was the control and the other groups were subjected to oral administration of either stone hair dye or para-phenylenediamine [PPD] in two doses [10 and 20 mg each]. The animals were sacrificed after 24 hours and haematological, histopathological [liver and kidney] and biochemical examinations were performed. The results obtained from the records revealed that 72.29% of the cases were from Qena, 14.45% from Aswan, 12% from Sohag and 1.2% from Assiut. The highest incidence of poisoning was found in 2006 [19.3%] followed by 2008, 2009 [15.7%] each, then 2004 and 2005 [13.3%], 2007 [12%], 2002 [7.2%] and lastly 2003 [3.6%]. The majority of victim's were females and most of cases were suicides. There were insignificant changes in RBCs count in all groups and insignificant decrease of Hb concentration while WBCs count was very significantly increased. The levels of AST and ALT showed a significant increase in all treated groups while serum level of creatinine was insignificantly decreased. There was significant increase in the plasma enzymes AST, GPT, the liver tissues showed many degenerative changes in the form of vacuolated cytoplasm and irregular deeply stained nuclei of the hepatocytes with vascular congestion and lymphocytic infiltration. The same results were obtained with both SHD and PPD. They were more obvious with high doses of both. The results confirmed that Para-phenylenediamine is the main toxic ingredient in stone hair dye, and the experimental study revealed that the liver is the target organ of para-phenylenediamine and SHD toxicity, also that toxic manifestations were dose related. This compound is highly toxic when taken by mouth and the outcome depends mainly on the dose taken. The study recommends that the sale and use of PPD containing dyes, SHD and henna should be prohibited

A clinical analysis of fetal chromosomal aberration induced by paint and hair dye / 南方医科大学学报

<p><b>OBJECTIVE</b>To analyze the effect of long-term exposure to paint or hair dye on chromosomal aberration of early embryos.</p><p><b>METHODS</b>We analyzed 2 cases of fetal or infantile chromosome aberration in which the parents experienced long-term exposure to paint and hair dye.</p><p><b>RESULT</b>The chromosomal mutations were detected in one 3-month-old infant and one 21-week-old fetus, and the karyotypes were 46,XX,del(2)(pter'q31) and 46,XX, t(4;12;15), respectively. Their parents worked with long-term exposure to paint and hair dye and developed such symptoms as dizziness, headache, and insomnia. The chromosomes of the parents remained normal, but the micronuclei of the lymphocytes and plasma lead level were increased with decreased WBC, platelet, and HGB.</p><p><b>CONCLUSION</b>Long exposure to paint or hair dye can cause poison and affect the normal growth of early embryos, leading eventually to gene and chromosomal mutation of the embryos.</p>

Exposições ambientais e padrões de distribuição de leucemias na infância / Environmental exposures and distribution patterns of leukemia in childhood

Objetivos: Analisar a tendência de mortalidade por leucemias na infância no Estado do Rio de Janeiro durante o período de 1980 a 2006. Método: Foram utilizados os dados de mortalidade por leucemia em menores 15 anos do Sistema de Informações de Mortalidade do Ministério da Saúde para os anos de 1980 a 2006, segundo sexo, dos residentes de três áreas: Município do Rio de Janeiro, Região Metropolitana (exceto município do Rio de Janeiro) e Interior do Estado. Foram considerados como óbitos por leucemia aqueles cuja causa básica havia sido codificada de acordo com a Classificação Internacional de Doença (CID) 9, no período 1980-95; segundo CID 10, no período 1996-2006. As taxas de mortalidade foram calculadas por faixa etária e ano de óbito, sendo em seguida ajustadas pela população mundial. Para análise de tendência, optou-se pelos modelos de regressão linear polinomial. Foi considerado um nível de significância de 5por cento. Resultados: As análises de tendência nas três localidades apresentaram perfis semelhantes, com um padrão decrescente e constante. Entretanto, o município do Rio de Janeiro apresentou a maior queda em suas taxas. Analisando a tendência das taxas de mortalidade por leucemia infantil segundo sexo, foi observado que no sexo masculino, a incidência foi maior quando comparada ao sexo feminino nas três localidades analisadas.Conclusão: Foi observada uma tendência de declínio da mortalidade por leucemias na infância no Estado do Rio de Janeiro, sendo esta mais acentuada na capital, do que na região metropolitana e no interior do Estado.

Myocardial damage in hair dye poisoning--an uncommon presentation.

A 22-year-old female presented to us after consuming hair dye with features of angio-neurotic edema, asphyxia and irregular pulse. Serial ECGs revealed presence of ST segment elevation and T wave inversion in anterior chest leads. Strongly positive c-troponin-T test further confirmed the myocardial damage. This is a rare manifestation of para-phenylene diamine poisoning mentioned in literature. Patient also had elevated liver enzymes and oliguria.

five years retrospective study of poisoning cases investigated by the forensic chemical laboratory in Upper Egypt

Poisoning is an important health hazard and one of the leading causes of morbidity and mortality worldwide. A five years retrospective study of poisoning cases in seven governorates of Upper Egypt investigated by Assiut Forensic Chemical Laboratory in the period from January 2002 to December 2006 was conducted to investigate the patterns, incidences, mode of poisoning, and types of poisons. The total number of cases was 407. The geographical distribution was; 14.0% from Almenia, 44.0% from Assiut, 19.9% from Sohag, 16.5% from Qena, 2.7% from Aswan, 2.2% from Red sea and 0.7% from New Valley. The highest incidence of poisoning was found in 2006 [29%] followed by 2004 [20.4%] then 2002 [18.7] and2005 [16.2%], lastly 2003 [15, 7%]. The highest incidence was in males [61.2%] and the maximum number of cases was recorded in the age group between 21 and 30 years [30.2%]. Suicide cases represented 49.6% of the total cases, 59.9% of them were females while cases of abuse represented 32.7% and 95.5% of them were males. Pesticides were the commonest poisons detected [47.3% of the total], where organophosphates represented [22.1%] and carbamates represented [20.6%] of the total cases. Also they were the main killer used in suicide cases in addition to hair dye ingestion [18.4%]. The use of hair dye for poisoning was common particularly in the south governorates [Qena 81.6% and Aswan 8.2%], either for suicide [83.7%] or trials of suicide [4.1%] and also for murder in 10.2% of the cases. Alcohol was the commonest substance of abuse reported in this study followed by antipsychotics [60.9% and 20.3% respectively]. Cannabis and hydrocarbons had similar percentages [5.3%] followed by hypnotics, opioids and other drugs. This study revealed that there is annual increase in the number of poisoning cases particularly in young adults. Deliberate self- poisoning is a major problem, followed by drug abuse. Pesticides were the main killer followed by hair dye. Alcohol was the commonest abuse substance. There was male predominance except in suicide cases where females predominate

Hair dye poisoning.

Hair dye ingestion is an uncommon form of poisoning in the west, however, in some parts of the world such as East Africa and Indian Sub-continent it is not uncommon. The main component of hair dye causing toxicity is Paraphenylenediamine (PPD). This compound has been found to cause angioneurotic edema, rhabdomyolysis and renal failure. We present a case of hair dye poisoning who presented with respiratory distress due to laryngeal edema and later developed trismus and carpopedal spasm. This case report highlights the combined toxicities of sodium EDTA and PPD.

Paraphenylene diamine ingestion: an uncommon cause of acute renal failure.

Paraphenylene diamine (PPD) is a major component of hair dyes. The aim is to study the renal manifestations and outcome of PPD consumption. During a four-year period from 2002 to February 2006, 10 persons were admitted to our Institute after consuming a hair dye in a suicidal bid. The percentage of ARF due to PPD at our Institute was 0.95%. Seven patients out of 10 (70%) who consumed PPD developed ARF. All 10 patients, including the patients who had normal renal function had features of rhabdomyolysis. Two patients required ventilator support for respiratory distress and two more required tracheostomy due to upper airway tract edema. One patient has expired after two sessions of dialysis. Renal biopsy in two patients (one, postmortem) showed acute tubular necrosis along with presence of casts in tubules due to myoglobin.

Occupational Skin Disease in Hairdressers: Results of Patch Test / 대한피부과학회지

BACKGROUND: Hairdressers are individuals at high risk of occupational skin disease, especially occupational allergic contact dermatitis (OACD), because of continuous exposure to water, detergents, hair dyes and bleaches, permanent wave solutions and metal equipment. OBJECTIVE: We evaluated the frequency of OACD among hairdressers and the sources of sensitization through patch test and questionnaires. METHODS: We analyzed the occupational characteristics (age, gender, duration of work), site of dermatitis, suspicious sensitizers, and use of preventive measures in a group of 96 hairdressers who answered a questionnaire. Among them, 61 hairdressers who were suspected of having occupational allergic contact dermatitis were patch-tested with the Korean standard series and hairdressing series. RESULTS: Among 96 hairdressers, 74 hairdressers (78%) responded with their experiences of work-related skin problems. The results of patch test revealed an occupationally-relevant sensitization in 82% of the 61 hairdressers. Mean frequencies of sensitization ranked as follows: nickel sulfate (37.7%), cobalt chloride (34.4%), paraphenylenediamine base (21.3%), thimerosal (11.5%), potassium dichromate (9.8%), mercury ammonium chloride (8.2%), Balsam Peru (6.6%), glyceryl monothioglycolate (4.9%), and fragrance mix (4.9%). We also classified these allergens as hairdressing agents, and hair dye showed the highest frequency of positive reactions (30%). CONCLUSION: From this study, we were able to unravel the important sensitizers related to the hairdressing profession. To improve the safety of their job and to the prevent occupational allergic contact dermatitis, hairdressers should be aware of these common sensitizers, and try to improve protective measures and their applications.