Expression of P53, HER2 and Ki67 proteins in rats subjected to bladder augmentation with stomach, colon and ileum

PURPOSE: To study the expression of HER2, p53 and Ki67 proteins in cystoplasties. METHODS: Sixty rats were distributed randomly into three groups of 20 animals. Bladder augmentation was held to increase with ileum (Group I), colon (Group II) and stomach (Group III). Tissue samples of neobladder was collected from each rat to its own control. The animals were sacrificed after 12 weeks. The neobladder was withdrawn for immunohistochemitry analysis of p53, HER2 and Ki67 expression. Wilcoxon and Mann-Whitney tests were used for statistical study. RESULTS: There were no significant changes in the expression of p53 and HER2 proteins. It was observed significant increase (p<0.0001) in Ki67 expression in all groups, when compared with their respective controls. When the study groups were compared with each other, there was increase of cell proliferation in the largest gastrocystoplasties in respect of ileocystoplasties (p=0.004) and colocystoplasties (p=0.003). CONCLUSION: We observed significant increase of cell proliferation characterized by Ki67 protein in the digestive tract of the ileocystoplasties, the colocystoplasties and the gastrocystoplasties and this increase was significantly greater in gastrocystoplasties.

Historia de la coloproctología: con especial referencia en Tucumán / History coloproctology: with particular reference in Tucumán

En este trabajo, en la primera parte se hacen algunas referencias sobre la Proctología o Coloproctología, como se la designa actualmente, a lo largo de la historia del hombre y a continuación tras una breve síntesis del desarrollo de la misma en Argentina realizamos un análisis del inicio y progreso de la especialidad en Tucumán destacando la figura del Dr. Julio U. Leites a quien consideramos el iniciador de la especialidad en Tucumán, objetivo principal de este trabajo. Finalizamos el mismo describiendo el posterior desarrollo de la coloproctología en la Sala 1 del Hospital Ángel C. Padilla.

In the first section of this report some facts about the history of Coloproctology are been referred. Afterwards through the story of mankind, a brief summary of the development in Argentine of the specialization and its competences is done. We have mentioned and analyze the beginning and progress of this branch of the surgery in Tucuman, remarking of Dr. Julio U. Leites figure, who is the very first surgeon in practice Proctology seriously and professionally. The last part of the article is to describe the beginning of Coloproctology at Angel C. Padilla Hospital.