RESUMO
Trichomonas vaginalis (T. vaginalis), the etiologic agent of human trichomoniasis, is a flagellated protozoan parasite, has been associated sith advese pregnancy outcomes, HIV transmission, and infertilityh. A total of one hundred and fifty-seven (157) women at childbearing age (14-49 years), were included in the presnt study, eighty six (86) symptomatic fertile while the other seventy-one (71) were infertile with or without sumptoms attending the Gynecology outpatient Department in Al-Emamayn Al-Kadhimayn Medical City, the High Institute of Infertility Diagnosis and Assisted Reproductive Technoligies at Al-Nahrain University in Baghdad, the maternity Teaching hospital, and Dr. Khawer center for infertility and IVF in Erbil province in Iraq. Two vaginal swab specimens were obtained from each of them:; one swab was immediately examined by wet mount microscopy, the other swab for molecular study (DNA extraction and p3 nested PCR). One hundred (100) samples positive in one or more test were identified: 20 (12.7%) infecions were detected by wet mount microscopy, while nested PCR was positive in 100 (63.7%) samples. These positive samples were seguenced and phylogenetic tree were done and, there was no association between the variations in glut (p3) gene of T. vaginalis isolated from infected women (fertile and infertile)
Assuntos
Humanos , Feminino , Gravidez , Adolescente , Adulto , Complicações na Gravidez/etiologia , Manejo de Espécimes/classificação , Tricomoníase/etiologia , Trichomonas vaginalis/genética , Alelos , Fertilidade , Glutaminase/genética , Infertilidade FemininaRESUMO
In the present study, vaginal discharge samples obtained from 207 patients with vaginal discharge complaints were examined by utilizing wet mount preparations, Giemsa staining method and CPLM cultivation method. In 27 of them [13.1%], T. vaginalis was detected by both saline wet mount preparation and Giemsa staining method. But as a result of the cultivation in CPLM media of the vaginal discharge materials collected from infected samples, reproduction was observed in only 21 of them [10.2%]. A treatment of secnidazole by 2 g unique dose was applied to infected patients and their spouses and a complete recovery was followed up both microscopically and clinically