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1.
Rev. bras. parasitol. vet ; 21(2): 97-100, Apr.-June 2012. tab
Artigo em Inglês | LILACS | ID: lil-643114

RESUMO

The present study aimed to assess potential changes in acute phase proteins in sheep experimentally infected with Trypanosoma vivax. There were studied eight male sheep, four used as controls and four infected with 10(5) T. vivax trypomastigotes. Blood samples were collected at two points times before infection and then at 5,7, 9, 11, 13, 15, 20, 30, 45, 60, 75, 90, 105 and 120 days post-infection (dpi). Blood samples were centrifuged and allotted, and acute phase proteins were then separated by electrophoresis on acrylamide gel containing sodium dodecyl sulfate. Protein concentrations were determined by computer-assisted densitometry. Total protein was determined by colorimetric biuret method. Trypanosomes were counted daily using a 5 mL aliquot of blood smear on a glass slide under a 22 × 22 mm coverslip. Parasites were counted in 100 microscopic fields (40× magnification), and then multiplied by a correction factor. The results were expressed as parasites per mL of blood. For statistical analyses, we used the Wilcoxon test at 5% significance level. There was found a reduction in several acute phase proteins and increase in antitrypsin and transferrin. This finding can be used for the diagnosis of T. vivax infection, especially in chronic infection.


O objetivo do presente estudo foi verificar possíveis alterações nas proteínas de fase aguda em ovinos infectados experimentalmente com Trypanosoma vivax. Para tanto, foram utilizados oito ovinos machos, sendo quatro usados como controle e quatro infectados com 10(5) tripomastigotas de T. vivax. Colheram-se amostras de sangue em dois tempos antes da infecção e, posteriormente, aos 5, 7, 9, 11, 13, 15, 20, 30, 45, 60, 75, 90, 105 e 120 dias após a infecção (dpi); após centrifugação e aliquotização das amostras. As proteínas de fase aguda foram separadas por eletroforese em gel de acrilamida, contendo dodecil sulfato de sódio, e suas concentrações foram determinadas através de densitometria computadorizada. A dosagem de proteína total foi realizada pelo método colorimétrico do biureto. A contagem dos tripanossomas foi realizada diariamente, utilizando-se uma alíquota de 5 µL de sangue disperso em lâmina de microscopia, sob lamínula de 22 × 22 mm, contando-se os parasitos em 100 campos microscópicos, com objetiva de 40×, multiplicados pelo fator de correção do microscópio, e o resultado expresso em parasitos por mL de sangue. Para a análise estatística, empregou-se o teste de Wilcoxon a 5% de probabilidade. Foi observada a diminuição de diversas proteínas de fase aguda e aumento de antitripsina e transferrina que podem ser utilizadas para auxiliar no diagnóstico da infecção por T. vivax, principalmente na fase crônica da infecção.


Assuntos
Animais , Masculino , Proteínas de Fase Aguda/análise , Doenças dos Ovinos/sangue , Doenças dos Ovinos/diagnóstico , Trypanosoma vivax , Tripanossomíase/veterinária , Doença Crônica , Ovinos , Tripanossomíase/sangue , Tripanossomíase/diagnóstico
2.
In. Carvalheiro, José da Rocha; Azevedo, Nara; Araújo-Jorge, Tania C. de; Lannes-Vieira, Joseli; Klein, Lisabel. Clássicos em doença de Chagas: história e perspectivas no centenário da descoberta. Rio de Janeiro, Fiocruz, 2009. p.51-122, ilus.
Monografia em Português | LILACS | ID: lil-535957

RESUMO

Revisões históricas aos avanços científicos para o controle da doença, o Simpósio Internacional Comemorativo do Centenário da Descoberta da Doença de Chagas (1909-2009).


Assuntos
Animais , Tripanossomíase/parasitologia , Tripanossomíase/patologia , Tripanossomíase/transmissão , História da Medicina , Caracteres Sexuais , Tripanossomíase/etiologia , Tripanossomíase/metabolismo , Tripanossomíase/sangue
3.
Iranian Journal of Arthropod-Borne Diseases. 2008; 2 (2): 48-51
em Inglês | IMEMR | ID: emr-101119

RESUMO

Trypanosoma are extra-erythrocytic, protozoan parasites of domestic and wild animals. It is transmitted by biting flies and a cause of fever, anemia, myocarditis and corneal opacity. In the present study, Trypanosoma infection is reported in a male bull dog of two years age at Lahore, Pakistan. Clinical signs and symptoms of infection are recorded. Confirmation of case was done by microscopic examination of Trypanosoma organism in thick blood smear. Furthermore, their effect on certain hematological parameters was studied and concluded that there was decrease of hemoglobin [Hb] concentration and packed cell volume [PCV] while erythrocytes sedimentation rate [ESR] was increased in the infected dog. The animal was successfully treated with single dose of diminazene diaceturate at the dose rate of 3.5 mg/kg body weight, intramuscularly alongwith the supportive therapy


Assuntos
Animais , Tripanossomíase/sangue , Tripanossomíase/tratamento farmacológico , Cães/parasitologia , /análogos & derivados , Trypanosoma , Hemoglobinas , Hematócrito , Sedimentação Sanguínea
5.
J Environ Biol ; 2001 Jan; 22(1): 75-7
Artigo em Inglês | IMSEAR | ID: sea-113611

RESUMO

The trypanosomes presence has been found to decrease blood ascorbic acid levels in fishes, Clarias batrachus and Heteropneustus fossilis by 55.7% and 54.70% respectively. The infection also showed significant increase in serum aldolase level of three fishes by 50.19% and 48.06% respectively.


Assuntos
Animais , Ácido Ascórbico/sangue , Doenças dos Peixes/sangue , Peixes , Água Doce , Frutose-Bifosfato Aldolase/sangue , Tripanossomíase/sangue
6.
Southeast Asian J Trop Med Public Health ; 1990 Mar; 21(1): 21-7
Artigo em Inglês | IMSEAR | ID: sea-31589

RESUMO

Trypanosoma evansi is the parasitic protozoon that causes "Surra", a wasting disease of domestic animals. Detection of T. evansi plays an important role in epidemiology and animal health. DNA probes were constructed from T. evansi genomic DNA and kinetoplast DNA for sensitive detection of the parasite in infected blood. A 6.5 kb DNA insert of pMUT ec6 plasmid derived from the genomic DNA of T. evansi Npl isolate, selected from 575 recombinant E. coli exhibited the strongest nucleic acid hybridization signal to the T. evansi DNA. Using as the DNA probe, pMUT ec6 could detect as little as 60 pg T. evansi DNA and it did not hybridize to the DNA of cattle, waterbuffalo and two related blood parasites. A simple detection procedure by spotting 10 microliters infected blood onto nylon membrane could sense as little as 1000 parasites. The kinetoplast DNA was cloned in E. coli and found to show a comparable sensitivity to that of the pMUT ec6. However, the kinetoplast DNA exhibited variation in copy number among parasite isolates thus pMUT ec6 should be the DNA probe of choice for sensitive detection of T. evansi.


Assuntos
Animais , Sangue/parasitologia , Sondas de DNA , Camundongos , Hibridização de Ácido Nucleico , Plasmídeos/genética , Tailândia , Trypanosoma/isolamento & purificação , Tripanossomíase/sangue
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