Compromiso neurológicoy cardiovascularen pacientes con triquinosis / Neurological and cardiovascular involventin patients with trichinosis

Introducción: La triquinosis es una infección parasitaria causada por nematodos del género Trichinella. El compromiso cardíaco no es habitual, pero representa la causa más frecuente de muerte por triquinosis, mientras que la afectación neurológica ocurre principalmente en pacientes severamente enfermos. Se sabe que a mayor cantidad de larvas ingeridas menor es el tiempo de incubación y mayor es la severidad de la enfermedad. Se presentan dos pacientes con compromiso del sistema nervioso central, uno de ellos cardiovascular, ambos pertenecientes a un brote de triquinosis ocurrido en la ciudad de Bahía Blanca.

Background: Trichinosis is a parasitic infection caused by nematodes of the genus Trichinella. Cardiac involvement is not usual but represents the most frequent cause of death by trichinosis, while neurological affectation occurs mainly in severely ill patients. A greater number of larvae ingested, the shorter the incubation time and the greater the severity of the disease. We present two patients with compromise of the central nervous system and one of them cardiovascular, both belonging to an outbreak of trichinosis in the city of Bahía Blanca

Evaluación de la infección de Trichinella spiralis en cerdos gonadectomizados, Zacatecas, México / An assessment of Trichinella spiralis infection in gonadectomized pigs in Zacatecas, Mexico

Objetivo: Evaluar la infección de Trichinella spiralis en cerdos gonadectomizados. Material y método: El presente trabajo se realizó utilizando 9 cerdos de la raza York de 4 meses de edad divididos en tres grupos: 3 cerdos control sanos, 3 cerdos infectados con Trichinella spiralis y 3 cerdos gonadectomizados y posteriormente infectados con Trichinella spiralis, todos los animales fueron sangrados pretratamiento y postratamiento para detección de anticuepos anti T. spiralis por las técnicas inmunologicas, Wester Blot y Inmunofluorescencia indirecta. Los animales fueron sacrificados a las 6 semanas de infección por Trichinella spiralis, se obtuvo tejido para las técnicas directas de compresión en placa y digestión artificial para evaluación de carga parasitaria. Resultados: en los cerdos gonadectomizados e infectados se presentó un ataque agudo de Trichinellosis, la carga parasitaria fue mayor estadísticamente significativa en relación a los cerdos infectados con P < 0.01), por el método de ANOVA y sin gonadectomizar, las técnicas inmunológicas fueron positivas en ambos grupos infectados y gonadectomizados. Conclusión: El diagnóstico oportuno de Trichinella spiralis mediante la evaluación directa y aletoria en camadas de cerdos a través de la observación de larva infectante en tejido cerebral, detección de anticuerpos anti - T. spiralis. por inmunofluorecescia indirecta e inmunoelectrotransferencia resultan eficaces y eficientes para hacer un diagnóstico oportuno en el animal para consumo humano.

Objective: To assess the prevalence of Trichinella spiralis in gonadectomized pigs. Material and methods: The study was performed using 9 four-month old pigs, York race, which were divided in three groups: 3 healthy control animals, 3 pigs infected with Trichinella spiralis, and 3 gonadectomized animals that later were infected with T. spiralis. Blood samples were taken from every animal before and after therapy for the detection of anti-T. spiralis antibodies using Western Blot and indirect immunofluorescence techniques. All animals were killed after 6 weeks of being infected with T. spiralis, and tissue samples were obtained for performing assessments using direct methods, such as plate compression and artificial digestion in order to look for the parasite load. Results: Gonadectomized and infected pigs developed an acute presentation of trichinellosis, their parasite load was statistically higher compared to infected non-gonadectomized pigs using ANOVA (P<0.01), and immune methods yielded positive results in both infected groups of animals. Conclusion: The timely diagnosis of Trichinella spiralis in pigs using direct and random assessments by observing the presence of infective worms in brain tissue, together with the detection of anti-T. spiralis antibodies using indirect immunofluorescence and immunoelectrotransference are both efficacious and efficient for the early diagnosis of this infection in animals whose meat is about to be consumed.

Effect of partial trasectomy on the stability of the precorneal tear film

The Precorneal tear film is formed of three components. We chose to study the most superficial oily layer which is secreted by the meibomian glands. Ten cases of trichiasis entropion were selected from the out-patient ophthalmic clinic of Ain Shams University Hospital during the year 1984. We did for every case before and after Snellen's operation [partial tarsectomy] Schirmer's tear that test No. 1 and tear film break up time [B.UT.]. The results were diminution of Schirmer's test No. 1 and Shortening of tear film break up time after Senllen's operation by two weeks. This indicates that the oily layer is important for stabilization of the precorneal tear film