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1.
Artigo em Inglês | IMSEAR | ID: sea-26055

RESUMO

The susceptibility of the newly established Ae. krombeini cell line (NIVI-AK-453) to six arboviruses, belonging to four different families, was studied. Sindbis (SIND), Vesicular stomatitis (VSV) Chandipura (CHP) and African horse sickness (AHS) viruses multiplied in these cultures. A four-to-five-fold increase in the virus titres was observed. The maximum titre of SIND, VSV, CHP and AHS viruses were observed on 1st, 4th, 3rd and 10th post infection days, respectively. A steady and significant increase in the titre of AHS was observed over a period of ten days. The sandfly fever virus (SFV) and the tick-borne, Kaisodi virus did not multiply in the cultures.


Assuntos
Aedes/microbiologia , Vírus da Doença Equina Africana/crescimento & desenvolvimento , Animais , Arbovírus/crescimento & desenvolvimento , Linhagem Celular , Rhabdoviridae/crescimento & desenvolvimento , Sindbis virus/crescimento & desenvolvimento , Vírus da Estomatite Vesicular Indiana/crescimento & desenvolvimento
2.
Indian J Biochem Biophys ; 1990 Dec; 27(6): 359-62
Artigo em Inglês | IMSEAR | ID: sea-28852

RESUMO

Administration of pyrene-linked fatty acids and lipids to cultured cells or an enveloped (vesicular stomatitis) virus induced photosensitization which, following irradiation with a long ultra-violet light (LUV), resulted in killing of the cells and loss of the infectivity of the virus with the following specific effects. (i) LUV illumination of the pyrene-sphingomyelin administered cultured skin fibroblasts derived from normal individuals and patients with Niemann-Pick disease permitted selective killing of the latter. (ii) Similarly LUV illumination of pyrenedodecanoic acid (P12) incubates of leukemic cell lines mixed with human bone marrow cells permitted selective killing of the former. (iii) LUV illumination of P12 incubates of vesicular stomatitis virus decreased the infectivity of the virus by up to 12 logs.


Assuntos
Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Fibroblastos , Ácidos Láuricos/farmacologia , Leucemia Mieloide/patologia , Doenças de Niemann-Pick/patologia , Pirenos/farmacologia , Esfingomielinas/farmacologia , Células Tumorais Cultivadas , Raios Ultravioleta , Vírus da Estomatite Vesicular Indiana/crescimento & desenvolvimento
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