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1.
Braz. j. infect. dis ; 11(2): 224-225, Apr. 2007.
Artigo em Inglês | LILACS | ID: lil-454723

RESUMO

This study aimed to test in vitro a RNA-interference based antiviral approach for rabies with short-interfering RNAs (siRNAs) against rabies virus nucleoprotein mRNA. BHK-21 cells were infected with serial dilutions of PV rabies virus strain and transfected with a pool of three siRNAs. Direct immunofluorescence staining showed a 5-time decrease in virus titer when compared to a non-treated plate, showing a promising new approach to the development of antivirals for rabies treatment.


Assuntos
Animais , Cricetinae , RNA Interferente Pequeno/uso terapêutico , Vírus da Raiva/genética , Replicação Viral/genética , Linhagem Celular , Imunofluorescência , RNA Mensageiro/genética , RNA Interferente Pequeno/genética , RNA Interferente Pequeno/metabolismo , RNA Viral/genética , Vírus da Raiva/crescimento & desenvolvimento , Coloração e Rotulagem
3.
Braz. j. med. biol. res ; 26(12): 1305-17, Dec. 1993. ilus, tab, graf
Artigo em Inglês | LILACS | ID: lil-148836

RESUMO

1. The rabies virus (Pasteur PV strain) was propagated in VERO cells attached to microcarriers in a 3.7-1 bioreactor. Virus titers of about 10(6) LD50/ml were obtained regularly. 2. Ultrafiltration was efficient for concentrating the virus suspensions, and the sucrose gradient reduced the residual VERO cell DNA to acceptable levels (less than 50 pg/dose). The remaining cell DNA content was evaluated by dot-blot hybridization with a probe prepared with VERO cell DNA. 3. The final virus preparations were inactivated by B-propiolactone treatment, showed a potency higher than 2.5 IU/dose and protected mice experimentally infected intracerebrally with rabies virus (CVS-13.2). 4. This methodology for the production of a rabies vaccine for human use should be of interest to countries where high technology facilities are not available


Assuntos
Humanos , Animais , Anticorpos Antivirais/imunologia , Vacina Antirrábica/imunologia , Vírus da Raiva/imunologia , DNA/análise , Immunoblotting , Hibridização de Ácido Nucleico , Fatores de Tempo , Vacinas de Produtos Inativados/imunologia , Células Vero , Vírus da Raiva/crescimento & desenvolvimento
4.
J Indian Med Assoc ; 1993 Jan; 91(1): 3-4
Artigo em Inglês | IMSEAR | ID: sea-95675
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