RESUMO
Cytomegalovirus (CMV) is a member of the Herpes virus group. It is a double stranded DNA virus and is known as Human Herpes Virus 5 in human hosts. CMV seropositivity is frequent in Western populations with a seroprevalence of 58% being reported in the U.S.1 In Africa, pooled prevalence studies demonstrate rates as high as 81.8% amongst HIV uninfected adults. Amongst the HIV infected population prevalence rates of 81.9% and 94.8% were seen in AIDS and asymptomatic HIV positive patients respectively.2 This is likely a reflection of impaired humoral immunity amongst those with AIDS. In a rural South African cohort, a 100% seroprevalence was demonstrated amongst HIV positive treatment naïve patients with a negative association noted between CD4 counts and IgG titres.3
Assuntos
Vacinas contra Citomegalovirus , África do SulRESUMO
Human cytomegalovirus is an important pathogen which widely infects human. In this study, an eukaryotic expression vector containing human cytomegalovirus gB680 and pp65m genes was injected into BALB/c mice to explore the immune response. Firstly, the recombinant plasmid pVAX1/gB680+pp65m and plasmid pVAX1 were transfected into COS-7 cells respectively and the transiently expressed product was detected by RT-PCR and Western blot. The maxiprepared plasmid pVAX1/gB680 + pp65m and pVAX1 by alkaline lysis with SDS and purified by Sepharose 4FF column chromatography were then used to immunize BALB/c mice and the humoral and cellular immune responses were determined. Recombinant plasmid pVAX1/gB680+pp65m could be expressed in COS-7 cells, and could induce antibodies and cellular immune responses in BALB/c mice.