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1.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wprim-811214

RESUMO

OBJECTIVE: The causal association of human papillomavirus (HPV) in uterine cervical cancer was well established and this oncogenic virus was reported to be a biomarker for overall recurrence and central pelvic recurrence. The objective of the present systematic review and meta-analysis was to assess the role of HPV DNA testing in early detection of recurrence among cervical cancer survivors after radiotherapy.METHODS: We performed a systematic review and meta-analysis by means of searching electronic databases for published articles between January 1984 and June 2018, on the basis of standard systematic review guidelines prescribed by major agencies namely Cochrane Collaboration (https://www.cochrane.org) and Campbell Collaboration (https://www.campbellcollaboration.org). The meta-analysis component was further modified appropriately for the synthesis of sensitivity and specificity results.RESULTS: A total of 1,055 cervical cancer cases who had received pelvic radiation with or without chemotherapy from ten cohort studies were evaluated. The overall pooled sensitivity and specificity of HPV DNA testing was 0.84 (95% confidence interval [CI]= 0.66–0.94) and 0.35 (95% CI=0.20–0.54) respectively. The positive likelihood ratio was 1.3 (95% CI=1.0–1.7) and the negative likelihood ratio was 0.45 (95% CI=0.18–1.10) with an estimated diagnostic odds ratio of 3 (95% CI=1–9).CONCLUSION: The screening for HPV DNA testing during follow-up facilitates early detection of recurrence after radiotherapy.


Assuntos
Colo do Útero , Estudos de Coortes , Comportamento Cooperativo , DNA , Tratamento Farmacológico , Feminino , Seguimentos , Testes de DNA para Papilomavírus Humano , Humanos , Programas de Rastreamento , Razão de Chances , Vírus Oncogênicos , Radioterapia , Recidiva , Sensibilidade e Especificidade , Sobreviventes , Neoplasias do Colo do Útero
2.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wprim-782088

RESUMO

Based on emerging data and current knowledge regarding high-risk human papillomavirus (hrHPV) testing as a primary screening for cervical cancer, the Korean Society of Obstetrics and Gynecology and the Korean Society of Gynecologic Oncology support the following scientific facts:• Compared to cytology, hrHPV screening has higher sensitivity and detects more cases of high-grade cervical intraepithelial neoplasia.• Qualified hrHPV testing can be considered as an alternative primary screening for cervical cancer to the current cytology method.• The starting age of primary hrHPV screening should not be before 25 years because of possible overtreatment in this age, which has a high human papillomavirus (HPV) prevalence but rarely progresses to cancer. The screening interval should be no sooner than every 3 years and no longer than every 5 years.• Before the introduction of hrHPV screening in Korea, research into comparative effectiveness of primary hrHPV screening for cervical cancer should be conducted to determine the appropriate HPV assay, starting age, and screening interval.


Assuntos
Neoplasia Intraepitelial Cervical , Detecção Precoce de Câncer , Ginecologia , Testes de DNA para Papilomavírus Humano , Humanos , Coreia (Geográfico) , Programas de Rastreamento , Sobremedicalização , Métodos , Obstetrícia , Prevalência , Neoplasias do Colo do Útero
3.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wprim-740191

RESUMO

OBJECTIVE: Human papillomavirus (HPV) infection is the most important risk factor for cervical cancer, which progresses from precursor lesions with no symptom if left untreated. We compared the risk of cervical dysplasia among HPV-positive Korean women based on HPV types and infection patterns. METHODS: We observed participants of a 5-year multicenter prospective cohort study, comprising HPV-positive women with either atypical squamous cells of undetermined significance or low-grade squamous intraepithelial lesion of the cervix at their enrollment. Follow-ups, comprising cytology and HPV DNA testing results, were included in the final analysis. Incidence was calculated for each infection pattern (persistent infection, incidental infection, and clearance). To investigate cervical dysplasia risk, we used Cox proportional hazard models adjusted for variables that were significantly different among infection patterns. From April 2010 to September 2017, 71 of 1,027 subjects developed cervical dysplasia more severe than high-grade squamous intraepithelial lesion of the cervix. RESULTS: Of these 71 subjects, persistent infection, incidental infection, and clearance were noted in 30, 39, and 2 individuals, respectively. Based on changes in DNA results during follow-up, cumulative incidence was 27.2%, 10.4%, and 0.5% for persistent infection, incidental infection, and clearance, respectively. Compared to clearance, the adjusted hazard ratios for cervical dysplasia were 51.6 and 24.1 for persistent and incidental infections, respectively (p < 0.001). CONCLUSION: Individuals persistently infected with the same HPV types during the follow-up period had the highest risk of severe cervical dysplasia. Hence, it is necessary to monitor HPV types and infection patterns to prevent severe cervical precancerous lesions.


Assuntos
Células Escamosas Atípicas do Colo do Útero , Colo do Útero , Estudos de Coortes , DNA , Feminino , Seguimentos , Testes de DNA para Papilomavírus Humano , Humanos , Incidência , Coreia (Geográfico) , Infecções por Papillomavirus , Modelos de Riscos Proporcionais , Estudos Prospectivos , República da Coreia , Fatores de Risco , Lesões Intraepiteliais Escamosas Cervicais , Displasia do Colo do Útero , Neoplasias do Colo do Útero
4.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wprim-766031

RESUMO

Since the introduction of the Papanicolaou (Pap) smear system in 1943, cervicovaginal cytology has been used as a standard screening test for cervical cancer. The dissemination of this test contributed to reductions of the incidence and mortality of cervical cancer worldwide. In Korea, regular health check-ups for industrial workers and their family members were introduced in 1988 and were performed as part of the National Cancer Screening Program in 1999. As a result, the incidence of cervical cancer in Korea has been steadily decreasing. However, about 800 cases of cervical cancer-related deaths are reported each year due to false-negative test results. Hence, new screening methods have been proposed. Liquid-based cytology (LBC) was introduced in 1996 to overcome the limitations of conventional Pap smears. Since then, other LBC methods have been developed and utilized, including the human papilloma virus test—a method with higher sensitivity that requires fewer screenings. In this study, we review current issues and future perspectives related to cervical cancer screening in Korea.


Assuntos
Colo do Útero , Detecção Precoce de Câncer , Feminino , Testes de DNA para Papilomavírus Humano , Humanos , Incidência , Coreia (Geográfico) , Programas de Rastreamento , Métodos , Mortalidade , Teste de Papanicolaou , Papillomaviridae , Neoplasias do Colo do Útero , Útero
5.
Rev. colomb. obstet. ginecol ; 69(3): 179-188, July-Sept. 2018. tab, graf
Artigo em Inglês | LILACS (Américas) | ID: biblio-978296

RESUMO

ABSTRACT Objective: To evaluate the usefulness of a device for collecting and preserving human papilloma virus (HPV) DNA in self-collected vaginal samples stored dry during 14 days. Materials and methods: Diagnostic concordance pilot study that included non-pregnant women over 25 years of age with a biopsy-confirmed result of cervical intraepithelial neoplasia (CIN) grade 1 or more, coming to two referral centres in Bogotá, Colombia. Women with a history of total hysterectomy were excluded. Convenience sampling was used. The device uses real-time PCR (polymerase chain reaction) for DNA detection. Sociodemographic and clinical variables were measured, as well as the results of the test when the sample was collected by the patient and when it was collected by the physician, and the amount of DNA in the samples taken and processed on day 1, and in those processed on day 14, using Ct thresholds. Descriptive statistics were applied. Overall concordance was estimated by means of the kappa coefficient and mean differences in DNA amount. Materials and methods: Diagnostic concordance pilot study that included non-pregnant women over 25 years of age with a biopsy-confirmed result of cervical intraepithelial neoplasia (CIN) grade 1 or more, coming to two referral centres in Bogotá, Colombia. Women with a history of total hysterectomy were excluded. Convenience sampling was used. The device uses real-time PCR (polymerase chain reaction) for DNA detection. Sociodemographic and clinical variables were measured, as well as the results of the test when the sample was collected by the patient and when it was collected by the physician, and the amount of DNA in the samples taken and processed on day 1, and in those processed on day 14, using Ct thresholds. Descriptive statistics were applied. Overall concordance was estimated by means of the kappa coefficient and mean differences in DNA amount. Results: A kappa coefficient of 0.84 (95% CI: 0.71-0.96) was found for concordance in high-risk HPV detection between the self-collected cervicovaginal sample and the sample taken by the clinician. There were no differences in terms of the amount of viral DNA between day 1 and day 14 (DM -0.34 cycles; 95% CI: - 2.29 to 1.61). Conclusion: Self-collected vaginal samples using the storage device are reliable for high-risk HPV detection in patients with cervical dysplasia, and preserve viral DNA for 14 days if stored dry at room temperature. Confirmation studies in the general population are required.


RESUMEN Objetivo: Evaluar la utilidad de un dispositivo para toma y preservación del DNA del virus del papiloma humano (VPH) de muestras vaginales recolectadas por autotoma y almacenadas en seco durante 14 días. Materiales y métodos: Estudio piloto de concordancia diagnóstica. Se incluyeron mujeres mayores de 24 años no gestantes con un resultado de neoplasia intraepitelial cervical (NIC) grado 1 o más, confirmado por biopsia en dos instituciones de referencia en Bogotá, Colombia. Se excluyeron mujeres con antecedente de histerectomía total. Se realizó un muestreo por conveniencia. El dispositivo utiliza PCR (reacción en cadena de la polimerasa) en tiempo real para detección del ADN. Se midieron variables sociodemográficas y clínicas, así como el resultado de la prueba por autotoma y tomada por el médico, y la cantidad de ADN de las muestras tomadas el día 1 procesadas ese día, y el día 14, por medio del Ct umbral. Se realizó estadística descriptiva. Se calculó la concordancia global por medio del índice de kappa ponderado y la diferencia de medias de la cantidad de ADN. Resultados: La concordancia en la detección de VPH de alto riesgo mostró un kappa = 0,84 (IC 95 %: 0,71-0,96) entre la muestra cervicovaginal recolectada por autotoma frente a la muestra cervical recolectada por el médico. No hubo diferencias en la cantidad de ADN viral entre el día 1 y el 14 (DM -0,34 ciclos; IC 95 %: -2,29 a 1,61). Conclusión: Las muestras vaginales recolectadas por autotoma usando el dispositivo de almacenamiento son confiables para la detección de VPH de alto riesgo en pacientes con displasia cervical, y preservan el ADN viral por 14 días si se almacenan en seco a temperatura ambiente. Se requieren estudios en población general para poder confirmar.


Assuntos
Humanos , Testes de DNA para Papilomavírus Humano , Manejo de Espécimes , Esfregaço Vaginal , Programas de Rastreamento , Autoexame
6.
An. bras. dermatol ; 93(1): 67-71, Jan.-Feb. 2018. tab, graf
Artigo em Inglês | LILACS (Américas) | ID: biblio-887140

RESUMO

Abstract: Background: In this study, cases with suspected urethral condylomata acuminata were examined by dermoscopy, in order to explore an effective method for clinical. Objective: To study the application of dermoscopy image analysis technique in clinical diagnosis of urethral condylomata acuminata. Methods: A total of 220 suspected urethral condylomata acuminata were clinically diagnosed first with the naked eyes, and then by using dermoscopy image analysis technique. Afterwards, a comparative analysis was made for the two diagnostic methods. Results: Among the 220 suspected urethral condylomata acuminata, there was a higher positive rate by dermoscopy examination than visual observation. Study limitations: Dermoscopy examination technique is still restricted by its inapplicability in deep urethral orifice and skin wrinkles, and concordance between different clinicians may also vary. Conclusion: Dermoscopy image analysis technique features a high sensitivity, quick and accurate diagnosis and is non-invasive, and we recommend its use.


Assuntos
Humanos , Masculino , Feminino , Pré-Escolar , Criança , Adolescente , Adulto , Pessoa de Meia-Idade , Adulto Jovem , Doenças Uretrais/diagnóstico por imagem , Condiloma Acuminado/diagnóstico por imagem , Dermoscopia/métodos , Doenças Uretrais/patologia , Condiloma Acuminado/patologia , Dermoscopia/instrumentação , Testes de DNA para Papilomavírus Humano , Confiabilidade dos Dados
7.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wprim-740170

RESUMO

OBJECTIVE: Knowledge regarding the prevalence and distribution of human papillomavirus (HPV) genotyping in healthy women is important in establishing strategies for cervical cancer screening and HPV vaccination. METHODS: A total of 18,170 women who visited a Korean Medical Institute for health check-ups were recruited retrospectively; they underwent HPV genotyping and conventional cervical cytology. An HPV DNA test was performed using the Anyplex™ II HPV 28 detection system (Seegene) or HPV Liquid Bead Microarray (Osang Healthcare). The distribution of HPV genotypes was assessed according to cervical cytology and age. RESULTS: HPV was detected in 3,037 (16.71%) of the 18,170 women enrolled, and 2,268 (12.48%) were positive for high-risk (HR) HPV. In total, HPV 53 (9.69% of all detected HPV viruses) was the most common type; HPV 58 (7.90%) and 52 (7.81%) were also common. HPV 54 (6.99%) was common in low-risk subjects. Overall and in the normal cytology group, the most common HPV genotype was HPV 53, whereas HPV 58 was more common in women who had atypical squamous cells of undetermined significance or low-grade squamous intraepithelial neoplasia cervical cytology. In addition, HPV 16 was the most common type in cases with high-grade squamous intraepithelial neoplasia (HSIL)/atypical squamous cells-cannot exclude HSIL. Among women with normal cytology, 76 of 231 (32.9%) women under 24 years of age were positive for HR HPV, whereas 84 of 852 (9.9%) women aged 55–59 years were positive. CONCLUSION: HPV 53 was the most prevalent genotype in healthy women. Distribution of HPV genotypes varied with cervical cytology and age. Our study provides important baseline data for the recently implemented national HPV vaccination program.


Assuntos
Células Escamosas Atípicas do Colo do Útero , Neoplasia Intraepitelial Cervical , Feminino , Genótipo , Papillomavirus Humano 16 , Testes de DNA para Papilomavírus Humano , Humanos , Programas de Rastreamento , Teste de Papanicolaou , Papillomaviridae , Prevalência , República da Coreia , Estudos Retrospectivos , Neoplasias do Colo do Útero , Vacinação
8.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wprim-718355

RESUMO

OBJECTIVE: This study was to identify the risk factors for cytological progression in women with atypical squamous cells of undetermined significance (ASC-US) or low-grade squamous intraepithelial lesions (LSIL). METHODS: We analyzed data from women infected with the human papillomavirus (HPV) who participated in the Korean HPV cohort study. The cohort recruited women aged 20–60 years with abnormal cervical cytology (ASC-US or LSIL) from April 2010. All women were followed-up at every 6-month intervals with cervical cytology and HPV DNA testing. RESULTS: Of the 1,158 women included, 654 (56.5%) and 504 (43.5%) women showed ASC-US and LSIL, respectively. At the time of enrollment, 143 women tested positive for HPV 16 (85 single and 58 multiple infections). Cervical cytology performed in the HPV 16-positive women showed progression in 27%, no change in 23%, and regression in 50% of the women at the six-month follow-up. The progression rate associated with HPV 16 infection was higher than that with infection caused by other HPV types (relative risk [RR], 1.75; 95% confidence interval [CI], 1.08–2.84; P=0.028). The cytological progression rate in women with persistent HPV 16 infection was higher than that in women with incidental or cleared infections (P < 0.001). Logistic regression analysis showed a significant relationship between cigarette smoking and cytological progression (RR, 4.15; 95% CI, 1.01–17.00). CONCLUSION: The cytological progression rate in HPV 16-positive women with ASC-US or LSIL is higher than that in women infected with other HPV types. Additionally, cigarette smoking may play a role in cytological progression.


Assuntos
Células Escamosas Atípicas do Colo do Útero , Estudos de Coortes , Epidemiologia , Feminino , Seguimentos , Papillomavirus Humano 16 , Testes de DNA para Papilomavírus Humano , Humanos , Modelos Logísticos , Papillomaviridae , Fatores de Risco , Fumar , Lesões Intraepiteliais Escamosas Cervicais
9.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wprim-715976

RESUMO

PURPOSE: High-risk human papillomavirus (HPV) infection progression should be considered a critical factor for preventing cervical cancer, although most infections are transient and rarely persist. This study aimed to examine the specific types of HPV infections, their change patterns, and the potential risk factors among Korean women. MATERIALS AND METHODS: We included 4,588 women who visited hospitals in Busan and Suwon for cervical cancer screening, and 1,224 of thesewomen attended a 2-yearfollow-up. Infection statuswas evaluated using HPV DNA testing (Hybrid Capture 2) and genotyping testing (Linear Array). Data regarding the potential risk factors for HPV infection were collected by trained nurses using structured questionnaires. RESULTS: Among the 1,224 women (mean age, 47 years), 105 women (8.6%) were HPV-positive at baseline. HPV infections had been cleared among 92 women (87.6%) within 2 years. Only 13 infections (12.4%) were remained, and the 10 cases of them are high-risk HPV types including genotype 33, 45, 16, 35, and 52. Among women who were negative at baseline, the HPV incidence was 4.8%. The HPV incidence was marginally associated with having multiple sexual partners (odds ratio, 2.0; 95% confidence interval, 1.0 to 3.9), although it was not significantly associated with HPV persistence. CONCLUSION: Most HPV infections (88%) among Korean women were cleared within 2 years, with only a small number of persistent infections. The persistent HPV genotypes were different in our study, compared to those from previous studies. Having multiple sexual partners was associated with acquiring a HPV infection, but not with persistence.


Assuntos
Feminino , Seguimentos , Genótipo , Testes de DNA para Papilomavírus Humano , Humanos , Incidência , Programas de Rastreamento , Fatores de Risco , Parceiros Sexuais , Neoplasias do Colo do Útero
10.
Cienc. Serv. Salud Nutr ; 8(1): 59-72, abr. 2017.
Artigo em Espanhol | LILACS (Américas) | ID: biblio-982228

RESUMO

La detección del cáncer cervicouterino mediante citología ha ido disminuyendo su incidencia en un 50%. Los métodos moleculares que detectan la presencia de HPV, consisten en la amplificación específica del DNA viral. La prueba de captura de Híbridos Hybrid Capture II (HC­II),(11), es un método semicuantitativo no radiactivo, basado en la hibridación del DNA de HPV con sondas de RNA, y en el que se usan anticuerpos monoclonales específicos que luego se revelan por quimioluminiscencia. A través de este método se detectaron cinco genotipos de bajo riesgo (6,11,42,43,44) y trece de alto riesgo (13,16,18,31,33,35,39,45,51,52,56,58,59 y 68). Objetivo: exponer test de HPV como método de cribado primario para diagnóstico temprano de genotipos de alto riesgo del HPV. Métodos: se realizó una revisión sobre el Test de virus papiloma humano como método de screening primario para el diagnóstico de neoplasias de cérvix uterino en Internet. La búsqueda abarcó artículos publicados en los últimos 10 años. Se consultaron las bases de datos de sistemas referativos, como MEDLINE, PubMed, Elsevier, Cochrane y SciELO con la utilización de varios descriptores tanto en inglés como en español. Se obtuvieron 27 artículos.


The detection of cervical cancer by cytology has been decreasing its incidence in a 50%. The molecular methods that detect the presence of HPV, consist of the specific amplification of viral DNA. The capture of Hybrid Capture II Hybrid (HC-II), (11), is a semi-quantitative non-radioactive method, based on the hybridization of HPV DNA with RNA probes, and in which specific monoclonal antibodies are used, are revealed by chemiluminescence. Five low-risk (6,11,42,43,44) and thirteen high-risk genotypes were detected through this method (13,16,18,31,33,35,39,45,51,52,56 , 58, 59 and 68) Objective: to present HPV test as a primary screening method for early diagnosis of HPV high-risk genotypes. Methods: a review was performed on the human papillomavirus test as a primary screening method for the diagnosis of uterine cervix neoplasms on the Internet. The search covered articles published in the last 10 years. Reference database databases such as MEDLINE, PubMed, Elsevier, Cochrane and SciELO were consulted using several descriptors in both English and Spanish. We obtained 27 articles.


Assuntos
Humanos , Feminino , Adolescente , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Adulto Jovem , Papillomaviridae , Neoplasias do Colo do Útero , Programas de Rastreamento , Testes de DNA para Papilomavírus Humano , Colo do Útero , Equador
12.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wprim-46651

RESUMO

OBJECTIVE: To determine whether triage for atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesion (LSIL) from the updated American Society for Colposcopy and Cervical Pathology cervical cancer screening guidelines is applicable in Korean women. METHODS: We investigated women with ASC-US or LSIL including referred from local hospitals visited for cervical cancer screening at Korea University Guro Hospital from February 2004 to December 2014. Detailed information on the results of Papanicolaou (Pap) smears, human papillomavirus (HPV) DNA tests, and cervical biopsies were collected through chart review. Cervical biopsy results were compared in eligible women according to individual Pap smear findings and HPV DNA status. RESULTS: Of 216,723 possible cases, 3,196 were included. There were 212 (6.6%) women with ASC-US and 500 (15.6%) with LSIL. The risk of ≥cervical intraepithelial neoplasia (CIN) 2 was significantly higher in women who were ASC-US/HPV+ than ASC-US/HPV- and LSIL/HPV+ than LSIL/HPV- (93.3% vs. 6.7% and 96.7% vs. 3.3%, P<0.001 and P<0.001, respectively). The risk of ≥CIN 3 was also significantly higher in women who were ASC-US/HPV+ than ASC-US/HPV- and LSIL/HPV+ than LSIL/HPV- (97.0% vs. 3.0% and 93.0% vs. 7.0%, P<0.001 and P<0.001, respectively). Age-stratified analysis revealed that more CIN 2 or CIN 3 was diagnosed in women aged 30 to 70 with ASC-US or LSIL when HPV DNA was present. CONCLUSION: Observation with Pap and HPV DNA tests rather than immediate colposcopy is a reasonable strategy for ASC-US or LSIL when the HPV DNA test is negative, especially in women aged 30 to 70. Reflection of these results should be considered in future Korean screening guidelines.


Assuntos
Células Escamosas Atípicas do Colo do Útero , Biópsia , Neoplasia Intraepitelial Cervical , Colposcopia , DNA , Feminino , Testes de DNA para Papilomavírus Humano , Humanos , Coreia (Geográfico) , Programas de Rastreamento , Teste de Papanicolaou , Papillomaviridae , Patologia , Lesões Intraepiteliais Escamosas Cervicais , Triagem , Neoplasias do Colo do Útero
13.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wprim-61160

RESUMO

OBJECTIVE: To explore the genotype distribution of high-risk human papillomavirus (HR-HPV) and its attribution to different grades of cervical lesions in rural China, which will contribute to type-specific HPV screening tests and the development of new polyvalent HPV vaccines among the Chinese population. METHODS: One thousand two hundred ninety-two subjects were followed based on the Shanxi Province Cervical Cancer Screening Study I (SPOCCS-I), and screened by HPV DNA testing (hybrid capture® 2 [HC2]), liquid-based cytology (LBC), and if necessary, directed or random colposcopy-guided quadrant biopsies. HPV genotyping with linear inverse probe hybridization (SPF10-PCR-LiPA) was performed in HC2 positive specimens. Attribution of specific HR-HPV type to different grades of cervical lesions was estimated using a fractional contribution approach. RESULTS: After excluding incomplete data, 1,274 women were included in the final statistical analysis. Fifteen point two percent (194/1,274) of women were HR-HPV positive for any of 13 HR-HPV types (HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, and 68) and the most common HR-HPV types were HPV16 (19.1%) and HPV52 (16.5%). The genotypes most frequently detected in HR-HPV-positive cervical intraepithelial neoplasia grade 1 (CIN1) were HPV52 (24.1%), HPV31 (20.7%), HPV16 (13.8%), HPV33 (13.8%), HPV39 (10.3%), and HPV56 (10.3%); in HR-HPV-positive cervical intraepithelial neoplasia grade 2 or worse (CIN2+): HPV16 (53.1%), HPV58 (15.6%), HPV33 (12.5%), HPV51 (9.4%), and HPV52 (6.3%). HPV52, 31, 16, 33, 39, and 56 together contributed to 89.7% of HR-HPV-positive CIN1, and HPV16, 33, 58, 51, and 52 together contributed to 87.5% of CIN2+. CONCLUSION: In summary, we found substantial differences in prevalence and attribution of CINs between different oncogenic HPV types in a rural Chinese population, especially for HPV16, 31, 33, 52, and 58. These differences may be relevant for both clinical management and the design of preventive strategies.


Assuntos
Grupo com Ancestrais do Continente Asiático , Biópsia , Neoplasia Intraepitelial Cervical , China , Feminino , Genótipo , Testes de DNA para Papilomavírus Humano , Humanos , Programas de Rastreamento , Vacinas contra Papillomavirus , Prevalência , Neoplasias do Colo do Útero
14.
DST j. bras. doenças sex. transm ; 28(4): 110-114, 20161220.
Artigo em Inglês | LILACS (Américas) | ID: biblio-868475

RESUMO

Cervical cancer is one of the most frequent cancers among Brazilian women and its relationship with the human papillomavirus (HPV) is well established. Objective: To analyze the presence of DNA/HPV using Hybrid Capture method for women in the city of Uruguaiana (RS). Methods: During the period of January to December 2015, 51 cervicovaginal samples were collected from patients who sought care at Basic Health Units in the city. After the collection, conventional and liquid-based cytological analysis was performed. Results: The results of the study indicate the prevalence of genital HPV infection in 5.9% of the samples; low-risk DNA/HPV was detected in 3.9% of patients of reproductive age (PIR); and 2.0% of PIR presented highrisk DNA/HPV. By stratifying the prevalence of HPV in age, we found positivity between 16 and 31 years. Conclusion: Conventional cytology is often inconclusive and, in such cases, using molecular biology methods that detect the DNA/HPV presence would be very useful.


O câncer de colo de útero é um dos mais frequentes entre as mulheres brasileiras e a sua relação com o Papilomavírus Humano (HPV) é bem estabelecida. Objetivo: Analisar a presença de DNA/HPV por meio do método de captura híbrida em mulheres no município de Uruguaiana (RS). Métodos: No período compreendido entre janeiro e dezembro de 2015, foram coletadas 51 amostras cervicovaginais de pacientes que buscaram atendimento nas Unidades Básicas de Saúde do município. Após a coleta, foi realizada a análise citológica convencional e em base líquida. Resultados: Os resultados encontrados no estudo indicam a prevalência de infecção genital por HPV em 5,9% das amostras analisadas, sendo DNA/HPV de baixo risco em 3,9% e DNA/HPV de alto risco em 2,0% das infecções. Ao estratificar a prevalência de HPV por faixa etária, observou-se positividade entre 16 e 31 anos. Conclusão: A citologia convencional pode ser, muitas vezes, inconclusiva e, nesses casos, utilizar uma metodologia de biologia molecular que detecte a presença do DNA/HPV seria muito útil.


Assuntos
Humanos , Feminino , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais , Infecções por Papillomavirus/diagnóstico , Neoplasias do Colo do Útero/diagnóstico , Testes de DNA para Papilomavírus Humano
15.
Cancer Research and Treatment ; : 1313-1320, 2016.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wprim-109745

RESUMO

PURPOSE: The purpose of this study is to evaluate the impact of high-risk human papillomaviruses (HPVs) other than HPV 16/18 on the natural course of atypical squamous cells of undetermined significance (ASC-US) or low-grade squamous intraepithelial lesion (LSIL). MATERIALS AND METHODS: The study population was derived from the Korean HPV cohort (2010-2014). Women aged 20 to 60 who satisfied the criteria of having both HPV infection and abnormal cervical cytology of either ASC-US or LSIL were recruited from five institutions nationwide. Enrolled patients underwent cervical cytology and HPV DNA testing every 6 months. RESULTS: A total of 1,158 patients were enrolled. The 10 most common HPV types were HPV 16 (12.3%), 58 (10.0%), 56 (8.8%), 53 (8.4%), 52 (7.7%), 39 (6.2%), 18 (6.0%), 51 (5.7%), 68 (5.1%), and 66 (4.6%). Among these patients, 636 women were positive for high-risk HPVs other than HPV 16 or 18, and 429 women were followed for more than 6 months. Cytology evaluations showed progression in 15.3% of women, no change in 22.6%, and regression in 62.1% of women at 12 months. In cases of HPV 58 single infection, a more highly significant progression rate, compared to other high-risk types, was observed at 6 months (relative risk [RR], 3.3; 95% confidence interval [CI], 2.04 to 5.30; p < 0.001) and 12 months (RR, 5.03; 95% CI, 2.56 to 9.91; p < 0.001). CONCLUSION: HPV genotypes numbered in the 50s were frequent in Korean women with ASC-US and LSIL. HPV 58 was the second most common type, with a high progression rate of cervical cytology.


Assuntos
Células Escamosas Atípicas do Colo do Útero , Estudos de Coortes , Feminino , Genótipo , Papillomavirus Humano 16 , Testes de DNA para Papilomavírus Humano , Humanos , Lesões Intraepiteliais Escamosas Cervicais
16.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wprim-182458

RESUMO

BACKGROUND: Geographic variation in the prevalence of carcinogenic types and human papillomavirus (HPV) genotype distribution is closely associated with the impact of HPV prophylactic vaccines. We determined the prevalence and distribution of HPV genotypes among healthy women in Korea. METHODS: This study included 968 healthy women who were examined at a health promotion center of the Korea University Guro Hospital between January and June 2013. Each participant had a Pap test and a HPV DNA test using the Anyplex™ II HPV 28 Detection system, which detects 19 high-risk HPVs (HR HPVs) and 9 low-risk HPVs (LR HPVs). Women with abnormal cytology and/or positivity for HR HPVs were referred to colposcopic biopsy. RESULTS: Overall HR HPV prevalence based on the assay was 33.7%. Among them, 225 women had single infection and 101 women had multiple infection. The most frequently occurring HR HPV types were 53 (6.5%), 52 (6.1%), 58 (4.8%), 16 (4.5%), and 68 (4.2%). The most frequently occurring LR HPV types were 54 (5.4%), 70 (3.8%), 42 (3.6%), 61 (3.4%), and 44 (3.1%). The prevalence of HPV 16 was highest (17.6%) among women with cervical intraepithelial neoplasia (CIN) and HPV 16 was strongly associated with a diagnosis of CIN2/3 (odds ratio = 20.5; 95% confidence interval: 3.9-107.1; P < 0.0001). CONCLUSIONS: HPV 53, 52, 58, 16, and 68 were common HR HPV types among healthy Korean women. HPV16 was the most common type in high-grade CIN lesions, as shown in most studies worldwide. The results might be useful information for cervical cancer prevention in South Korea.


Assuntos
Biópsia , Neoplasia Intraepitelial Cervical , Diagnóstico , Feminino , Genótipo , Promoção da Saúde , Papillomavirus Humano 16 , Testes de DNA para Papilomavírus Humano , Humanos , Coreia (Geográfico) , Teste de Papanicolaou , Prevalência , Neoplasias do Colo do Útero , Vacinas
17.
Braz. j. otorhinolaryngol. (Impr.) ; 81(6): 598-603, Nov.-Dec. 2015. tab
Artigo em Português | LILACS (Américas) | ID: lil-770204

RESUMO

ABSTRACT INTRODUCTION: Many epidemiological studies have suggested that human papillomavirus (HPV), especially type 16, is involved in the genesis of squamous cell carcinoma of the oral cavity and oropharynx, especially in young, non-smoking patients; thus, its detection in lesions in this region is important. OBJECTIVE: To clarify the capacity of the brushing sampling method to detect the presence of HPV in oral or oropharyngeal lesions through polymerase chain reaction (PCR) testing, and to compare the results with those obtained by biopsy. METHODS: Prospective study of adult patients with oral or oropharyngeal lesions assessed by PCR, comparing biopsy specimens with samples obtained by the brushing method. The study was approved by the Research Ethics Committee of the institution. RESULTS: A total of 35 sample pairs were analyzed, but 45.7% of the brushing samples were inadequate (16/35) and, thus, only 19 pairs could be compared. There was agreement of results in 94.7% (18/19) of the pairs, with HPV identified in 16 of them. HPV DNA was detected in 8.6% (3/35) of biopsy and 5.7% (2/35) of brushing samples. CONCLUSION: There was no statistically significant difference between the two methods, but the brushing sampling method showed a higher number of inadequate samples, suggesting that it is an unreliable method for surveillance.


Resumo INTRODUÇÃO: Muitos estudos epidemiológicos indicam a participação do papilomavírus humano, especialmente o tipo 16, na carcinogênese dos tumores espinocelulares das cavidade oral e oro-faríngea, principalmente em jovens e não fumantes, sendo portanto importante sua detecção nas lesões desta região. OBJETIVO: Elucidar a habilidade do escovado em detectar o papilomavírus humano, pela reação em cadeia da polimerase, nas lesões orais e orofaríngeas, comparando os resultados com os obtidos por biópsia. MÉTODO: Estudo prospectivo de pacientes com lesões orais e orofaríngeas, pela reação em cadeia da polimerase, no qual foram pareados os resultados de amostras obtidas por escovado e por biópsia. A pesquisa foi aprovada pelo Comitê de Ética em Pesquisa da instituição. RESULTADO: Foram analisados 35 pares de amostras, porém estavam inapropriadas para análise 45,7% (16/35) das amostras obtidas por escovado, e portanto, somente 19 pares puderam ser comparados. Em 94,7% dos pares houve concordância dos resultados, sendo encontrado o papilomavírus humano − 16 em um destes pares. O ácido desoxirribonucleico do papilomavírus humano foi detectado em 8,6% (3/35) das biópsias e em 5,7% (2/35) dos escovados. CONCLUSÃO: Não houve diferença estatística entre os métodos, mas como houve um grande número de amostras obtidas por escovado inapropriadas, este parece não ser confiável para o rastreamento.


Assuntos
Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Bucais/virologia , Neoplasias Orofaríngeas/virologia , Papillomaviridae/isolamento & purificação , Infecções por Papillomavirus/virologia , Biópsia/métodos , Estudos Transversais , DNA Viral/análise , Testes de DNA para Papilomavírus Humano , Neoplasias Bucais/diagnóstico , Neoplasias Orofaríngeas/diagnóstico , Orofaringe/virologia , Reação em Cadeia da Polimerase , Estudos Prospectivos , Papillomaviridae/genética , Infecções por Papillomavirus/patologia , Sensibilidade e Especificidade
18.
Arq. bras. med. vet. zootec ; 67(4): 1193-1196, July-Aug. 2015. tab
Artigo em Inglês | LILACS (Américas), VETINDEX | ID: biblio-1095960

RESUMO

A placa aural é uma dermatopatia associada à quatro Equus caballus papillomavirus (EcPVs). Até o momento, o DNA de EcPVs não foi identificado em amostras de placa aural fixadas em formalina e embebidas em parafina (FFPE). O objetivo deste estudo foi otimizar um método para a detecção dos quatro tipos de EcPVs em 21 amostras FFPE usando a PCR. O DNA dos EcPVs foram detectados em 11 amostras (52.4%). O DNA do EcPV4 foi detectado em 38.1% (8/21) e do EcPV3 em 4.8% (1/21) das amostras. Coinfecção foi identificada em duas amostras (9.5%); EcPV4 e 5 foram detectados simultaneamente em uma amostra, enquanto o DNA dos EcPV4 e 6 foi detectado em outra. A especificidade do DNA dos papilomavírus equinos foi avaliada por sequenciamento gênico direto, que confirmou a especificidade dos produtos. A metodologia de PCR proposta possibilita o diagnóstico dos EcPV3, 4, 5 e 6 em amostras FFPE de placa aural equina.(AU)


Assuntos
Animais , Métodos Analíticos de Preparação de Amostras/veterinária , Testes de DNA para Papilomavírus Humano/veterinária , Cavalos/virologia , Parafina , Reação em Cadeia da Polimerase/veterinária
19.
Artigo em Inglês | IMSEAR (Sudeste Asiático), GHL | ID: sea-162077

RESUMO

Introduction: Human papillomavirus (HPV) is a DNA virus which has tropism for epithelial cells, is the major etiological factor for development of cervical precancerous and cancerous lesions. Nearly 100 diff erent types of HPV have been characterized and thereare a large number of other types. HPV infection is one of the most common causes of sexually transmitted disease in both men and women worldwide. It is associated with a variety of clinical conditions that range from innocuous lesions to cancer. Genital HPV types are divided into high and low-risk types, according to the oncogenic potential. Molecular and epidemiologic studies have solidifi ed the association between high risk HPV types (especially HPV-16 and HPV-18) and cervical squamous cell carcinoma. HPV infection is often transient and self-limiting but infection may persists and progress to high grade lesions and cancer. In addition to persistent high-risk HPV infection, other viral factors such as high viral loads, HPV variants, infections with multiple high-risk HPV types and genetic predisposition contribute to the development of cervical cancer. Th e aim of the present study was to detect HPV DNA and identify high risk HPV genotype among women having cervical intraepithelial neoplasia and carcinoma and to evaluate potential effi cacy of prophylactic HPV vaccine. Methods: Cervical swab from histopathologically diagnosed CIN (n=51) and carcinoma (n=39) patients were taken and high risk HPV DNA was detected by HC II assay. Polymerase Chain Reaction was used to identify high risk HPV genotype. Result: HPV DNA was detected in 41 (45.56%) patients by HC II assay. HPV type 16 was detected in 27 (81.82%) followed by type 18 in 3 (9.09%) and type 45 in 2 (6.06%) cases of cervical carcinoma. Among precancerous cases, only type 16 was detected. Conclusion: Knowledge based on HPV prevalence and genotype could be used to predict the effi cacy of cost eff ective prophylactic vaccine, introduction of newer generation vaccine and management of cervical carcinoma.


Assuntos
Adulto , DNA Viral/genética , Feminino , Genótipo/genética , Técnicas de Genotipagem/métodos , Testes de DNA para Papilomavírus Humano/métodos , Humanos , Testes de DNA para Papilomavírus Humano/métodos , Papillomaviridae/genética , Lesões Pré-Cancerosas/epidemiologia , Lesões Pré-Cancerosas/genética , Risco , Neoplasias do Colo do Útero/epidemiologia , Neoplasias do Colo do Útero/genética , Adulto Jovem , Neoplasias do Colo do Útero/terapia
20.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wprim-36575

RESUMO

OBJECTIVE: Human papillomavirus (HPV) test was incorporated into the triage of lesser abnormal cervical cytologies: atypical squamous cells of undetermined significance (ASCUS) or low-grade squamous intraepithelial lesion (LSIL). This study aimed to evaluate the impact of age on the efficacy of HPV testing in patients with lesser abnormal cervical cytologies. METHODS: A total of 439 patients with ASCUS or LSIL were included. The association between age groups and the diagnostic performances of HPV test for high-grade cervical intraepithelial neoplasia (CIN2+) was evaluated. RESULTS: Median age was 44 years (range, 17 to 75 years). ASCUS was more frequently observed in older patients while LSIL was more common in younger patients (P=0.002). CIN2+ was found in 11.3% (32/284) of the ASCUS patients and 12.9% (20/155) of patients with LSIL. Older patients with ASCUS showed lower HPV infection rates (P=0.025), but not LSIL (P=0.114). However, the prevalence of CIN2+ was similar between the age groups with ASCUS or LSIL. In patients with ASCUS, the false negative rate of HPV test for CIN2+ was 6.2%. The false negative rate of the HPV test became higher with increasing of the age after the age of 50 (P=0.034). CONCLUSION: Our findings suggest that false negative rate of the HPV test for CIN2+ in ASCUS patients older than 50 years might become higher with increasing of the age. Negative HPV results in patients of the age >50 years with ASCUS should be carefully interpreted.


Assuntos
Neoplasia Intraepitelial Cervical , DNA , Testes de DNA para Papilomavírus Humano , Humanos , Prevalência , Triagem , Displasia do Colo do Útero
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