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A new organ preservation solution for static cold storage of the liver. Amniotic fluid
Büyük, Basak; Demirci, Tuba; Adali, Yasemen; Eroglu, Hüseyin Avni.
  • Büyük, Basak; Çanakkale Onsekiz Mart University. Faculty of Medicine. Department of Histology-Embryology. TR
  • Demirci, Tuba; Ataturk University. Faculty of Medicine. Department of Histology and Embryology. TR
  • Adali, Yasemen; Çanakkale Onsekiz Mart University. Faculty of Medicine. Department of Pathology. TR
  • Eroglu, Hüseyin Avni; Çanakkale Onsekiz Mart University. Faculty of Medicine. Department of Physiology. TR
Acta cir. bras ; 34(4): e201900402, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001091
ABSTRACT
Abstract

Purpose:

To evaluate the effect of amniotic fluid in liver preservation in organ transplantation, and compare it with standard preservation solutions.

Methods:

The groups consisted of Group 1 Ringer Lactate (RL) group, Group 2 HTK group, Group 3 UW group, Group 4 AF group. The livers of rats from Group 1, 2, 3, and 4 were perfused and placed into falcon tubes containing RL, HTK, UW, and AF solutions at +4‎°C, respectively. The tubes were stored for 12 hours in the refrigerator at +4°C. Tissue samples were taken at the 6th and 12th hours for histopathological examinations of the perfused livers, and storage solutions for biochemical analyzes at 6th and 12th hours.

Results:

AF was shown to maintain organ viability by reducing the number of cells undergoing apoptosis. Histopathological changes such as sinusoidal dilatation, hydropic degeneration, and focal necrosis were found to be similar to the groups in which the standard organ preservation solutions were used. Additionally, the results of INOS, IL-10, and TNF-α,which were evaluated immunohistochemically, have been shown to be similar to the UW and HTK groups.

Conclusions:

AF provided conservation similar to UW and HTK in the 12-hour liver SCS process. The fact that apoptosis values are comparable to standard preservation solutions supports the success of AF in the cold storage of the liver.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Cryopreservation / Organ Preservation Solutions / Amniotic Fluid / Liver Type of study: Evaluation studies Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article Affiliation country: Turkey Institution/Affiliation country: Ataturk University/TR / Çanakkale Onsekiz Mart University/TR

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Full text: Available Index: LILACS (Americas) Main subject: Cryopreservation / Organ Preservation Solutions / Amniotic Fluid / Liver Type of study: Evaluation studies Limits: Animals Language: English Journal: Acta cir. bras Journal subject: General Surgery / Procedimentos Cir£rgicos Operat¢rios Year: 2019 Type: Article Affiliation country: Turkey Institution/Affiliation country: Ataturk University/TR / Çanakkale Onsekiz Mart University/TR