Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content
Braz. arch. biol. technol
;
62: e19180178, 2019. tab, graf
Article
in English
| LILACS
| ID: biblio-1001423
ABSTRACT
Abstract We present an improved method of direct transesterification suitable for the quantitative analysis of multiple dry samples for its fatty acid content, using a minimal amount of biomass and reactants. The method features an acid-catalyzed direct alcoholysis of microgram samples of dry biomass; the rationale behind the solvent and reagent proportions chosen is discussed. The method was validated using seven microbial strains with diverse lipid content (Saccharomyces cerevisiae, Saccharomyces boulardii, Candida tropicalis, Haematococcus pluvialis, Chlorella vulgaris, Spirulina platensis and Schizochytrium limacinum), and compared with a macroscale direct transesterification method, and with gravimetric analysis of lipids extracted with solvents. The microscale method showed a conversion of 98.06 ± 0.87% of the lipids, using approximately 3 mg of dry biomass, 1mL of 0.2M H2SO4 dissolved in anhydrous ethanol (the acid is the catalyzer and ethanol the reactant)). The mixture was maintained at 70 °C for 20 h with periodic mixing, and then extracted with 2mL n-heptane and analyzed by GC-FID. The lipid content was then calculated considering dilution and sample mass. This method is effective, reliable, and technically attractive for analytical and comparative purposes.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Biomass
/
Ethanol
/
Fatty Acids
Type of study:
Diagnostic study
/
Screening study
Language:
English
Journal:
Braz. arch. biol. technol
Journal subject:
Biology
Year:
2019
Type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Federal University of Paraná/BR
/
Federal University of Technology/BR
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