Hydrogen sulfide protects H9c2 cardiomyoblasts against H2O2-induced apoptosis
Braz. j. med. biol. res
; 52(4): e7626, 2019. graf
Article
in En
| LILACS
| ID: biblio-1001516
Responsible library:
BR1.1
ABSTRACT
Reactive oxygen species (ROS) are highly reactive chemical species that may cause irreversible tissue damage, and play a critical role in cardiovascular diseases. Hydrogen sulfide (H2S) is a gasotransmitter that acts as a ROS scavenger with cardio-protective effects. In this study, we investigated the cytoprotective effect of H2S against H2O2-induced apoptosis in cardiomyocytes. H9c2 rat cardiomyoblasts were treated with H2S (100 μM) 24 h before challenging with H2O2 (100 μM). Apoptosis was then assessed by annexin V and PI, and mitochondrial membrane potential was measured using a fluorescent probe, JC-1. Our results revealed that H2S improved cell viability, reduced the apoptotic rate, and preserved mitochondrial membrane potential. An increased Bcl-2 to Bax ratio was also seen in myocytes treated with H2S after H2O2-induced stress. Our findings indicated a therapeutic potential for H2S in preventing myocyte death following ischemia/reperfusion.
Key words
Full text:
1
Index:
LILACS
Main subject:
Apoptosis
/
Myoblasts, Cardiac
/
Hydrogen Peroxide
/
Antioxidants
Type of study:
Evaluation_studies
Limits:
Animals
Language:
En
Journal:
Braz. j. med. biol. res
Journal subject:
BIOLOGIA
/
MEDICINA
Year:
2019
Type:
Article