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Artesunate promotes Th1 differentiation from CD4+ T cells to enhance cell apoptosis in ovarian cancer via miR-142
Chen, Xiao; Zhang, Xue-ling; Zhang, Guo-hua; Gao, Ying-fang.
  • Chen, Xiao; Fourth Hospital of Shijiazhuang. Department of Gynecology and Obstetrics. Shijiazhuang. CN
  • Zhang, Xue-ling; Fourth Hospital of Shijiazhuang. Department of Gynecology and Obstetrics. Shijiazhuang. CN
  • Zhang, Guo-hua; Fourth Hospital of Shijiazhuang. Department of Gynecology and Obstetrics. Shijiazhuang. CN
  • Gao, Ying-fang; Fourth Hospital of Shijiazhuang. Department of Gynecology and Obstetrics. Shijiazhuang. CN
Braz. j. med. biol. res ; 52(5): e7992, 2019. graf
Article in English | LILACS | ID: biblio-1001527
ABSTRACT
The aim of this study was to evaluate the influence of artesunate on Th1 differentiation and its anti-tumor effect on ovarian cancer. A Murine ovarian cancer model was established by ID8 cells transplantation. The expression of miR-142 and Sirt1 proteins in peripheral CD4+ T cells were quantified with qRT-PCR and western blot, respectively. Peripheral CD4+ T cells were induced for Th1 differentiation. The percentages of apoptosis of Th1/CD4+ T cells and ovarian cancer cells were analyzed by flow cytometry. The IFN-γ level was examined through enzyme-linked immunosorbent assay. Artesunate promoted miR-142 expression in peripheral CD4+ T cells and Th1 differentiation from CD4+ T cells. Artesunate promoted cell apoptosis of ovarian cancer cells by inducing Th1 differentiation. By up-regulating miR-142, artesunate suppressed Sirt1 level and promoted Th1 differentiation. Artesunate enhanced the pro-apoptotic effects of Th1 cells on ovarian cancer via the miR-142/Sirt1 pathway. Artesunate promoted Th1 differentiation from CD4+ T cells by down-regulating Sirt1 through miR-142, thereby enhancing cell apoptosis in ovarian cancer.
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Full text: Available Index: LILACS (Americas) Main subject: Ovarian Neoplasms / CD4-Positive T-Lymphocytes / Apoptosis / Th1 Cells / MicroRNAs / Artesunate Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Fourth Hospital of Shijiazhuang/CN

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Full text: Available Index: LILACS (Americas) Main subject: Ovarian Neoplasms / CD4-Positive T-Lymphocytes / Apoptosis / Th1 Cells / MicroRNAs / Artesunate Limits: Animals Language: English Journal: Braz. j. med. biol. res Journal subject: Biology / Medicine Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: Fourth Hospital of Shijiazhuang/CN