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Comparison of two rotary systems in bacteria/lps removal from endodontic infections: randomized clinical trial
Endodontic DivisionMachado, Camila Ambrósio Dias; Endodontic DivisionSouza, Amanda Caselato Andolfatto; Endodontic DivisionLoureiro, Caroline; Martinho, Frederico Canato; Endodontic DivisionCintra, Luciano Tavares Ângelo; Endodontic DivisionDezan Junior, Eloi; Endodontic DivisionJacinto, Rogério de Castilho.
  • Endodontic DivisionMachado, Camila Ambrósio Dias; Universidade Estadual Paulista - Unesp. School of Dentistry. Endodontic DivisionMachado, Camila Ambrósio Dias. São Paulo. BR
  • Endodontic DivisionSouza, Amanda Caselato Andolfatto; Universidade Estadual Paulista - Unesp. School of Dentistry. Endodontic DivisionSouza, Amanda Caselato Andolfatto. São Paulo. BR
  • Endodontic DivisionLoureiro, Caroline; Universidade Estadual Paulista - Unesp. School of Dentistry. Endodontic DivisionLoureiro, Caroline. São Paulo. BR
  • Martinho, Frederico Canato; University of Maryland. Baltimore School of Dentistry. Baltimore. US
  • Endodontic DivisionCintra, Luciano Tavares Ângelo; Universidade Estadual Paulista - Unesp. School of Dentistry. Endodontic DivisionCintra, Luciano Tavares Ângelo. São Paulo. BR
  • Endodontic DivisionDezan Junior, Eloi; Universidade Estadual Paulista - Unesp. School of Dentistry. Endodontic DivisionDezan Junior, Eloi. São Paulo. BR
  • Endodontic DivisionJacinto, Rogério de Castilho; Universidade Estadual Paulista - Unesp. School of Dentistry. Endodontic DivisionJacinto, Rogério de Castilho. São Paulo. BR
Braz. oral res. (Online) ; 33: e039, 2019. tab, graf
Article in English | LILACS | ID: biblio-1001610
ABSTRACT
Abstract This clinical study compared the effectiveness of two rotary systems HyFlex CM (Coltene-Whaledent, Altstetten, Switzerland) and ProTaper Next (Dentsply Sirona, Ballaigues, Switzerland) on the removal of cultivable bacteria and endotoxins from primarily infected root canals. This study was designed as a randomized single-blinded, 2-arm, clinical trial. Twenty-four primarily infected root canals were selected and randomly divided into 2 groups HyFlex CM (n = 12); and ProTaper Next (n = 12). Samples were collected before and after the biomechanical preparation and inoculated in specific flasks. Irrigation was performed using 2.5% sodium hypochlorite. A kinetic turbidimetric lysate assay of limulus amoebocytes was used to quantify endotoxins. Microbiological culture technique was used to determine the count of bacterial colony forming units (CFU/mL). Data collected were statistically analyzed using SigmaPlot 12.0 for Windows. The Two-Way ANOVA statistical test was performed and the level of significance was 5%. In the samples before the biomechanical preparation, cultivable bacteria and endotoxins were evidenced in 100% of the cases. The culture analysis revealed that there was no statistically significant difference in the bacterial reduction between the two instrumentation systems. Endotoxins were present in 100% of the canals after instrumentation and there was no statistical difference between the two systems in endotoxin reduction. Thus, it was concluded that both instrumentation systems were effective in reducing root canal bacteria and endotoxins with primary endodontic infection and that there was no statistical difference between them. However, no system was able to eliminate 100% of the bacteria and their by-products.
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Full text: Available Index: LILACS (Americas) Main subject: Bacteria / Lipopolysaccharides / Dental Instruments / Dental Pulp Cavity Type of study: Controlled clinical trial Limits: Adult / Female / Humans / Male Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Brazil / United States Institution/Affiliation country: Universidade Estadual Paulista - Unesp/BR / University of Maryland/US

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Full text: Available Index: LILACS (Americas) Main subject: Bacteria / Lipopolysaccharides / Dental Instruments / Dental Pulp Cavity Type of study: Controlled clinical trial Limits: Adult / Female / Humans / Male Language: English Journal: Braz. oral res. (Online) Journal subject: Dentistry Year: 2019 Type: Article Affiliation country: Brazil / United States Institution/Affiliation country: Universidade Estadual Paulista - Unesp/BR / University of Maryland/US