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Id-1 expression in colorectal adenocarcinoma tissues and its clinical significance
Wu, Xue-Liang; Wang, Yuan-Yuan; Wang, Li-Kun; Xue, Jun; Yang, Dong-Dong; Qu, Ming; Wang, Chen-Yu; Guo, Fei; Yang, Rui-Min; Liu, Bo.
  • Wu, Xue-Liang; First Affiliated Hospital of Hebei North University. Department of General Surgery. Zhangjiakou. CN
  • Wang, Yuan-Yuan; The First Hospital of Hebei Medical University. Department of Gastrointestinal Surgery. Shijiazhuang. CN
  • Wang, Li-Kun; First Affiliated Hospital of Hebei North University. Department of Ultrasound. Zhangjiakou. CN
  • Xue, Jun; First Affiliated Hospital of Hebei North University. Department of General Surgery. Zhangjiakou. CN
  • Yang, Dong-Dong; First Affiliated Hospital of Hebei North University. Department of General Surgery. Zhangjiakou. CN
  • Qu, Ming; First Affiliated Hospital of Hebei North University. Department of General Surgery. Zhangjiakou. CN
  • Wang, Chen-Yu; First Affiliated Hospital of Hebei North University. Department of General Surgery. Zhangjiakou. CN
  • Guo, Fei; First Affiliated Hospital of Hebei North University. Department of General Surgery. Zhangjiakou. CN
  • Yang, Rui-Min; First Affiliated Hospital of Hebei North University. Department of Ultrasound. Zhangjiakou. CN
  • Liu, Bo; First Affiliated Hospital of Hebei North University. Department of Pathology. Zhangjiakou. CN
Rev. Assoc. Med. Bras. (1992) ; 65(3): 404-409, Mar. 2019. graf
Article in English | LILACS | ID: biblio-1003052
Responsible library: BR1.1
ABSTRACT
SUMMARY

BACKGROUND:

This study aims to investigate the expression of Id-1 in human colorectal adenocarcinoma tissues and explore its correlation with the clinical pathological parameters of colorectal cancer.

METHODS:

The Id-1 mRNA and protein expression levels of 50 specimens of normal colorectal tissues and 50 specimens of colorectal adenocarcinoma tissues were detected using reverse-transcription polymerase chain reaction and western blot. Furthermore, Id-1 protein was detected using immunohistochemistry. The correlation between the expression of Id-1 and clinicopathologic features was analyzed.

RESULTS:

The mRNA expression level of Id-1 in colorectal adenocarcinoma tissues and normal colorectal tissues was 0.96 ± 0.03 vs. 0.20 ± 0.04, respectively; and the difference was statistically significant (P=0.011). Furthermore, Id-1 protein expression was higher in colorectal adenocarcinoma tissues than in normal colorectal tissues (0.82 ± 0.04 vs. 0.31 ± 0.02, P=0.020). In addition, the positive protein expression rate of Id-1 was higher in colorectal adenocarcinoma tissues than in normal colorectal tissues (72.00% vs. 24.00%, X2=23.431, P=0.000). The expression of Id-1 was correlated with the depth of tumor invasion, TNM stage, lymph node metastasis, vessel invasion, and liver metastasis (P<0.01). However, this expression was not correlated with tumor size and differentiation degrees (P>0.05).

CONCLUSIONS:

The high Id-1 expression in colorectal adenocarcinoma tissues play an important role in the process of cancer, and is expected to become a new tumor monitoring indicator for clinical diagnosis, treatment, and prognosis judgment.
RESUMO
RESUMO

OBJETIVO:

O objetivo deste estudo é investigar a expressão de Id-1 em tecidos de adenocarcinoma colorretal em humanos e investigar sua correlação com os parâmetros patológicos clínicos de câncer colorretal.

MÉTODOS:

Os níveis de expressão de proteína e mRNA Id-1 em 50 amostras de tecido colorretal normal e 50 amostras de tecido de adenocarcinoma colorretal foram detectados através de reação em cadeia de polimerase precedida de transcrição reversa e western blot. Além disso, a proteína Id-1 foi detectada através de imuno-histoquímica. A correlação entre a expressão de Id-1 e características clínico-patológicas foi analisada.

RESULTADOS:

O nível de expressão de mRNA Id-1 em tecidos de adenocarcinoma colorretal e tecidos colorretais normais foi de 0,96 ± 0,03 versus 0,20 ± 0,04, respectivamente; a diferença foi estatisticamente significativa (P= 0,011). Além disso, a expressão da proteína Id-1 foi maior em tecidos de adenocarcinoma colorretal do que em tecidos colorretais normais (0,82 ± 0,04 versus 0,31 ± 0,02, P= 0,020). Além disso, a taxa de expressão positiva de proteínas Id-1 foi maior em tecidos de adenocarcinoma colorretal do que em tecidos colorretais normais (72,00% vs. 24,00%, X2=23,431, p=0,000). A expressão de Id-1 foi correlacionada com a profundidade da invasão tumoral, estágio TNM, metástases linfonodais, invasão vascular e metástase hepática (P<0,01). Todavia, essa expressão não se correlacionou com o tamanho do tumor e graus de diferenciação (P>0,05).

CONCLUSÃO:

A alta expressão de Id-1 em tecidos de adenocarcinoma colorretal desempenham um importante papel no processo do câncer, e é esperado que se torne um novo indicador de monitoramento de tumores para o diagnóstico clínico, tratamento e estimativa de prognóstico.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Colorectal Neoplasms / Adenocarcinoma / Inhibitor of Differentiation Protein 1 Limits: Adult / Aged / Female / Humans / Male Language: English Journal: Rev. Assoc. Med. Bras. (1992) Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: First Affiliated Hospital of Hebei North University/CN / The First Hospital of Hebei Medical University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Colorectal Neoplasms / Adenocarcinoma / Inhibitor of Differentiation Protein 1 Limits: Adult / Aged / Female / Humans / Male Language: English Journal: Rev. Assoc. Med. Bras. (1992) Year: 2019 Type: Article Affiliation country: China Institution/Affiliation country: First Affiliated Hospital of Hebei North University/CN / The First Hospital of Hebei Medical University/CN