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Efficient immobilization of agarase using carboxyl-functionalized magnetic nanoparticles as support
Xiao, Anfeng; Xiao, Qiong; Lin, Yan; Ni, Hui; Zhu, Yanbing; Cai, Huinong.
  • Xiao, Anfeng; Jimei University. Xiamen. CN
  • Xiao, Qiong; Jimei University. Xiamen. CN
  • Lin, Yan; Jimei University. College of Chengy. Xiamen. CN
  • Ni, Hui; Jimei University. Xiamen. CN
  • Zhu, Yanbing; Jimei University. Xiamen. CN
  • Cai, Huinong; Jimei University. Xiamen. CN
Electron. j. biotechnol ; 25: 13-20, ene. 2017. ilus, graf
Article in English | LILACS | ID: biblio-1008291
ABSTRACT

Background:

A simple and efficient strategy for agarase immobilization was developed with carboxyl-functionalized magnetic nanoparticles (CMNPs) as support. The CMNPs and immobilized agarase (agarase-CMNPs) were characterized by transmission electron microscopy, dynamic light scattering, vibrating sample magnetometry, scanning electron microscopy, X-ray diffraction, thermogravimetric analysis, and zeta-potential analysis. The hydrolyzed products were separated and detected by ESI-TOF-MS.

Results:

The agarase-CMNPs exhibited a regular spherical shape with a mean diameter of 12 nm, whereas their average size in the aqueous solution was 43.7 nm as measured by dynamic light scattering. These results indicated that agarase-CMNPs had water swelling properties. Saturation magnetizations were 44 and 29 emu/g for the carriers and agarase-CMNPs, respectively. Thus, the particles had superparamagnetic characteristics, and agarase was successfully immobilized onto the supports. Agaro-oligosaccharides were prepared with agar as substrate using agarase-CMNPs as biocatalyst. The catalytic activity of agarase-CMNPs was unchanged after six reuses. The ESI-TOF mass spectrogram showed that the major products hydrolyzed by agarase-CMNPs after six recycle uses were neoagarotetraose, neoagarohexaose, and neoagarooctaose. Meanwhile, the end-products after 90 min of enzymatic treatment by agarase-CMNPs were neoagarobiose and neoagarotetraose.

Conclusions:

The enhanced agarase properties upon immobilization suggested that CMNPs can be effective carriers for agarase immobilization. Agarase-CMNPs can be remarkably used in developing systems for repeated batch production of agar-derived oligosaccharides.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Oligosaccharides / Enzymes, Immobilized / Magnetite Nanoparticles / Glycoside Hydrolases Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2017 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Jimei University/CN

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Full text: Available Index: LILACS (Americas) Main subject: Oligosaccharides / Enzymes, Immobilized / Magnetite Nanoparticles / Glycoside Hydrolases Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2017 Type: Article / Project document Affiliation country: China Institution/Affiliation country: Jimei University/CN