Construction of an RNAi vector for knockdown of GM-ACS genes in the cotyledonary nodes of soybean
Electron. j. biotechnol
;
26: 40-45, Mar. 2017. ilus, graf
Article
in English
| LILACS
| ID: biblio-1009000
ABSTRACT
Background:
Ethylene plays an important role in the regulation of floral organ development in soybean, and 1-aminocyclopropane-1-carboxylate synthase (ACS) is a rate-limiting enzyme for ethylene biosynthesis. However, whether ACS also regulates floral organ differentiation in soybean remains unknown. To address this, we constructed an RNAi vector to inhibit ACS expression in cotyledonary nodes. Linear DNA cassettes of RNAi-ACS obtained by PCR were used to transform soybean cotyledonary nodes.Results:
In total, 131 of 139 transiently transformed plants acquired herbicide resistance and displayed GUS activities in the new buds. In comparison to untransformed seedling controls, a greater number of flower buds were differentiated at the cotyledonary node; GM-ACS1 mRNA expression levels and ethylene emission in the transformed buds were reduced.Conclusion:
These results indicate that the cotyledonary node transient transformation system may be suitable for stable transformation and that the inhibition of ACS expression may be an effective strategy for promoting floral organ differentiation in soybean.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Soybeans
/
RNA Interference
/
Lyases
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2017
Type:
Article
/
Project document
Affiliation country:
China
Institution/Affiliation country:
Jilin Normal University/CN
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