Highly efficient enzymatic preparation of isomalto-oligosaccharides from starch using an enzyme cocktail
Electron. j. biotechnol
;
26: 46-51, Mar. 2017. graf, tab
Article
in English
| LILACS
| ID: biblio-1009650
ABSTRACT
Background:
Current commercial production of isomalto-oligosaccharides (IMOs) commonly involves a lengthy multistage process with low yields.Results:
To improve the process efficiency for production of IMOs, we developed a simple and efficient method by using enzyme cocktails composed of the recombinant Bacillus naganoensis pullulanase produced by Bacillus licheniformis, α-amylase from Bacillus amyloliquefaciens, barley bran ß-amylase, and α-transglucosidase from Aspergillus niger to perform simultaneous saccharification and transglycosylation to process the liquefied starch. After 13 h of reacting time, 49.09% IMOs (calculated from the total amount of isomaltose, isomaltotriose, and panose) were produced.Conclusions:
Our method of using an enzyme cocktail for the efficient production of IMOs offers an attractive alternative to the process presently in use.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Oligosaccharides
/
Starch
/
Enzymes
/
Isomaltose
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2017
Type:
Article
/
Project document
Affiliation country:
China
/
South Africa
Institution/Affiliation country:
Durban University of Technology/ZA
/
Fuzhou University/CN
/
Tianjin University of Science and Technology/CN
Similar
MEDLINE
...
LILACS
LIS