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Highly efficient enzymatic preparation of isomalto-oligosaccharides from starch using an enzyme cocktail
Niu, Dandan; Qiao, Jian; Li, Pujun; Tian, Kangming; Liu, Xiaoguang; Singh, Suren; Lu, Fuping.
  • Niu, Dandan; Fuzhou University. College of Biological Science and Engineering. Fujian Provincial Key Laboratory of Marine Enzyme Engineering. Fuzhou. CN
  • Qiao, Jian; Tianjin University of Science and Technology. The College of Biotechnology. Tianjin. CN
  • Li, Pujun; Tianjin University of Science and Technology. The College of Biotechnology. Tianjin. CN
  • Tian, Kangming; Tianjin University of Science and Technology. College of Chemical Engineering and Material Sciences. Department of Biological Chemical Engineering. Tianjin. CN
  • Liu, Xiaoguang; Tianjin University of Science and Technology. College of Chemical Engineering and Material Sciences. Department of Biological Chemical Engineering. Tianjin. CN
  • Singh, Suren; Durban University of Technology. Department of Biotechnology and Food Technology. Durban. ZA
  • Lu, Fuping; Tianjin University of Science and Technology. The College of Biotechnology. Tianjin. CN
Electron. j. biotechnol ; 26: 46-51, Mar. 2017. graf, tab
Article in English | LILACS | ID: biblio-1009650
ABSTRACT

Background:

Current commercial production of isomalto-oligosaccharides (IMOs) commonly involves a lengthy multistage process with low yields.

Results:

To improve the process efficiency for production of IMOs, we developed a simple and efficient method by using enzyme cocktails composed of the recombinant Bacillus naganoensis pullulanase produced by Bacillus licheniformis, α-amylase from Bacillus amyloliquefaciens, barley bran ß-amylase, and α-transglucosidase from Aspergillus niger to perform simultaneous saccharification and transglycosylation to process the liquefied starch. After 13 h of reacting time, 49.09% IMOs (calculated from the total amount of isomaltose, isomaltotriose, and panose) were produced.

Conclusions:

Our method of using an enzyme cocktail for the efficient production of IMOs offers an attractive alternative to the process presently in use.
Subject(s)


Full text: Available Index: LILACS (Americas) Main subject: Oligosaccharides / Starch / Enzymes / Isomaltose Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2017 Type: Article / Project document Affiliation country: China / South Africa Institution/Affiliation country: Durban University of Technology/ZA / Fuzhou University/CN / Tianjin University of Science and Technology/CN

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Full text: Available Index: LILACS (Americas) Main subject: Oligosaccharides / Starch / Enzymes / Isomaltose Language: English Journal: Electron. j. biotechnol Journal subject: Biotechnology Year: 2017 Type: Article / Project document Affiliation country: China / South Africa Institution/Affiliation country: Durban University of Technology/ZA / Fuzhou University/CN / Tianjin University of Science and Technology/CN