Utilization of a waste glycerol fraction using and reusing immobilized Gluconobacter oxydans ATCC 621 cell extract
Electron. j. biotechnol
;
27: 44-48, May. 2017. tab
Article
in English
| LILACS
| ID: biblio-1010289
ABSTRACT
Background:
Depletion of petroleum resources has enforced the search for alternative sources of renewable energy. Introduction of biofuels into the market was expected to become a solution to this disadvantageous situation. Attempts to cover fuel demand have, however, caused another severe problemthe waste glycerol generated during biodiesel production at a concentration of approximately 10% w/w. This, in turn, prompted a global search for effective methods of valorization of the waste fraction of glycerol.Results:
Utilization of the waste fraction at 48 h with an initial glycerol concentration of 30 g·L-1 and proceeding with 62% efficiency enabled the production of 9 g·L-1 dihydroxyacetone at 50% substrate consumption. The re-use of the immobilized biocatalyst resulted in a similar concentration of dihydroxyacetone (8.7 g·L-1) in two-fold shorter time, with an efficiency of 85% and lower substrate consumption (35%).Conclusions:
The method proposed in this work is based on the conversion of waste glycerol to dihydroxyacetone in a reaction catalyzed by immobilized Gluconobacter oxydans cell extract with glycerol dehydrogenase activity, and it could be an effective way to convert waste glycerol into a valuable product.
Full text:
Available
Index:
LILACS (Americas)
Main subject:
Cells, Immobilized
/
Dihydroxyacetone
/
Glycerol
Language:
English
Journal:
Electron. j. biotechnol
Journal subject:
Biotechnology
Year:
2017
Type:
Article
Affiliation country:
Poland
Institution/Affiliation country:
Warsaw University of Life Sciences/PL
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